Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the domin...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Mary Ann Liebert, Inc.
2019
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| Materias: | |
| Acceso en línea: | https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062 http://hdl.handle.net/20.500.12123/5063 http://doi.org/10.1089/fpd.2011.1062 |
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| author | Del Castillo, Lourdes D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta |
| author_browse | Carbonari, Claudia Carolina Chinen, Isabel Del Castillo, Lourdes Deza, Natalia D’Astek, Beatriz A. Leotta, Gerardo A. Manfredi, Eduardo Masana, Marcelo Oscar Miliwebsky, Elizabeth Palladino, Pablo Martin Rivas, Marta |
| author_facet | Del Castillo, Lourdes D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta |
| author_sort | Del Castillo, Lourdes |
| collection | INTA Digital |
| description | Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No
correlation was found among the stx-genotypes, the phage type, and the clinical symptoms. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5063 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Mary Ann Liebert, Inc. |
| publisherStr | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | INTA50632019-05-08T11:18:37Z Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina Del Castillo, Lourdes D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta Escherichia coli Ganado Bovino Cattle Animal Diseases Identification Enfermedades de los Animales Identificación Argentina Escherichia coli O157:H7 Strains Isolated Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms. Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. 2019-05-08T11:05:38Z 2019-05-08T11:05:38Z 2012-05-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062 http://hdl.handle.net/20.500.12123/5063 1535-3141 1556-7125 http://doi.org/10.1089/fpd.2011.1062 eng info:eu-repo/semantics/restrictedAccess application/pdf Mary Ann Liebert, Inc. Foodborne Pathogens and Disease 9 (5) : 457-464 (2012) |
| spellingShingle | Escherichia coli Ganado Bovino Cattle Animal Diseases Identification Enfermedades de los Animales Identificación Argentina Escherichia coli O157:H7 Strains Isolated Del Castillo, Lourdes D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
| title | Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
| title_full | Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
| title_fullStr | Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
| title_full_unstemmed | Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
| title_short | Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
| title_sort | subtyping of escherichia coli o157 h7 strains isolated from human infections and healthy cattle in argentina |
| topic | Escherichia coli Ganado Bovino Cattle Animal Diseases Identification Enfermedades de los Animales Identificación Argentina Escherichia coli O157:H7 Strains Isolated |
| url | https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062 http://hdl.handle.net/20.500.12123/5063 http://doi.org/10.1089/fpd.2011.1062 |
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