Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed no...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/5048 https://jfoodprotection.org/doi/pdf/10.4315/0362-028X.JFP-11-189 https://doi.org/10.4315/0362-028X.JFP-11-189 |
| _version_ | 1855483545483476992 |
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| author | Masana, Marcelo Oscar D’Astek, Beatriz A. Palladino, Pablo Martin Galli, Lucia Del Castillo, Lourdes Carbonari, Claudia Carolina Leotta, Gerardo A. Vilacoba, E. Irino, K. Rivas, Marta |
| author_browse | Carbonari, Claudia Carolina Del Castillo, Lourdes D’Astek, Beatriz A. Galli, Lucia Irino, K. Leotta, Gerardo A. Masana, Marcelo Oscar Palladino, Pablo Martin Rivas, Marta Vilacoba, E. |
| author_facet | Masana, Marcelo Oscar D’Astek, Beatriz A. Palladino, Pablo Martin Galli, Lucia Del Castillo, Lourdes Carbonari, Claudia Carolina Leotta, Gerardo A. Vilacoba, E. Irino, K. Rivas, Marta |
| author_sort | Masana, Marcelo Oscar |
| collection | INTA Digital |
| description | The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease. |
| format | Artículo |
| id | INTA5048 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | INTA50482019-06-10T17:54:47Z Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina Masana, Marcelo Oscar D’Astek, Beatriz A. Palladino, Pablo Martin Galli, Lucia Del Castillo, Lourdes Carbonari, Claudia Carolina Leotta, Gerardo A. Vilacoba, E. Irino, K. Rivas, Marta Escherichia coli Carne de Res Mataderos Beef Abattoirs Genotypes Genotipos Genotypic Non-O157 Shiga Toxin Beef Abattoirs The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease. Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: D’astek, B.A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Galli, Lucia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Del Castillo, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Carbonari, C. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Leotta, G. A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Vilacoba, E. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Irino, K. Instituto Adolfo Lutz. Secao de Bacteriologia; Brasil. Fil: Rivas, M. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. 2019-05-07T11:27:27Z 2019-05-07T11:27:27Z 2011-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5048 https://jfoodprotection.org/doi/pdf/10.4315/0362-028X.JFP-11-189 0362-028X 1944-9097 https://doi.org/10.4315/0362-028X.JFP-11-189 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Food Protection 74 (12 ): 2008–2017 (2011) |
| spellingShingle | Escherichia coli Carne de Res Mataderos Beef Abattoirs Genotypes Genotipos Genotypic Non-O157 Shiga Toxin Beef Abattoirs Masana, Marcelo Oscar D’Astek, Beatriz A. Palladino, Pablo Martin Galli, Lucia Del Castillo, Lourdes Carbonari, Claudia Carolina Leotta, Gerardo A. Vilacoba, E. Irino, K. Rivas, Marta Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina |
| title | Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina |
| title_full | Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina |
| title_fullStr | Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina |
| title_full_unstemmed | Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina |
| title_short | Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina |
| title_sort | genotypic characterization of non o157 shiga toxin producing escherichia coli in beef abattoirs of argentina |
| topic | Escherichia coli Carne de Res Mataderos Beef Abattoirs Genotypes Genotipos Genotypic Non-O157 Shiga Toxin Beef Abattoirs |
| url | http://hdl.handle.net/20.500.12123/5048 https://jfoodprotection.org/doi/pdf/10.4315/0362-028X.JFP-11-189 https://doi.org/10.4315/0362-028X.JFP-11-189 |
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