Cooperativity in proton sensing by PIP aquaporins

One of the most intriguing properties of plasma membrane intrinsic protein (PIP) aquaporins (AQPs) is their ability to modulate water transport by sensing different levels of intracellular pH through the assembly of homo‐ and heterotetrameric molecular species in the plasma membrane. In this work, u...

Full description

Bibliographic Details
Main Authors: Vitali, Victoria Andrea, Jozefkowicz, Cintia, Canessa Fortuna, Agustina, Soto, Gabriela Cinthia, Gonzalez Flecha, Francisco Luis, Alleva, Karina Edith
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Wiley 2019
Subjects:
Online Access:https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.14701
http://hdl.handle.net/20.500.12123/5031
https://doi.org/10.1111/febs.14701
_version_ 1855035426663825408
author Vitali, Victoria Andrea
Jozefkowicz, Cintia
Canessa Fortuna, Agustina
Soto, Gabriela Cinthia
Gonzalez Flecha, Francisco Luis
Alleva, Karina Edith
author_browse Alleva, Karina Edith
Canessa Fortuna, Agustina
Gonzalez Flecha, Francisco Luis
Jozefkowicz, Cintia
Soto, Gabriela Cinthia
Vitali, Victoria Andrea
author_facet Vitali, Victoria Andrea
Jozefkowicz, Cintia
Canessa Fortuna, Agustina
Soto, Gabriela Cinthia
Gonzalez Flecha, Francisco Luis
Alleva, Karina Edith
author_sort Vitali, Victoria Andrea
collection INTA Digital
description One of the most intriguing properties of plasma membrane intrinsic protein (PIP) aquaporins (AQPs) is their ability to modulate water transport by sensing different levels of intracellular pH through the assembly of homo‐ and heterotetrameric molecular species in the plasma membrane. In this work, using a phenomenological modeling approach, we demonstrate that cooperativity in PIP biological response cannot be directly attributed to a cooperative proton binding, as it is usually considered, since it could also be the consequence of a cooperative conformation transition between open and closed states of the channel. Moreover, our results show that, when mixed populations of homo‐ and heterotetrameric PIP channels are coexpressed in the plasma membrane of the same cell, the observed decrease in the degree of positive cooperativity would result from the simultaneous presence of molecular species with different levels of proton sensing. Indeed, the random mixing between different PIP paralogues as subunits in a single tetramer, plus the possibility of mixed populations of homo‐ and heterotetrameric PIP channels widen the spectrum of cooperative responses of a cell membrane. Our approach offers a deep understanding of cooperative transport of AQP channels, as members of a multiprotein family where the relevant proton binding sites of each member have not been clearly elucidated yet.
format info:ar-repo/semantics/artículo
id INTA5031
institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2019
publishDateRange 2019
publishDateSort 2019
publisher Wiley
publisherStr Wiley
record_format dspace
spelling INTA50312019-05-03T18:43:53Z Cooperativity in proton sensing by PIP aquaporins Vitali, Victoria Andrea Jozefkowicz, Cintia Canessa Fortuna, Agustina Soto, Gabriela Cinthia Gonzalez Flecha, Francisco Luis Alleva, Karina Edith Plant Water Relations Cell Membranes Relaciones Planta Agua Membranas Celulares Aquaporin Acuaporinas One of the most intriguing properties of plasma membrane intrinsic protein (PIP) aquaporins (AQPs) is their ability to modulate water transport by sensing different levels of intracellular pH through the assembly of homo‐ and heterotetrameric molecular species in the plasma membrane. In this work, using a phenomenological modeling approach, we demonstrate that cooperativity in PIP biological response cannot be directly attributed to a cooperative proton binding, as it is usually considered, since it could also be the consequence of a cooperative conformation transition between open and closed states of the channel. Moreover, our results show that, when mixed populations of homo‐ and heterotetrameric PIP channels are coexpressed in the plasma membrane of the same cell, the observed decrease in the degree of positive cooperativity would result from the simultaneous presence of molecular species with different levels of proton sensing. Indeed, the random mixing between different PIP paralogues as subunits in a single tetramer, plus the possibility of mixed populations of homo‐ and heterotetrameric PIP channels widen the spectrum of cooperative responses of a cell membrane. Our approach offers a deep understanding of cooperative transport of AQP channels, as members of a multiprotein family where the relevant proton binding sites of each member have not been clearly elucidated yet. Instituto de Genética Fil: Vitali, Victoria Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Ciudad Universitaria. Instituto de Química y Fisicoquímica Biológica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Canessa Fortuna, Agustina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Ciudad Universitaria. Instituto de Química y Fisicoquímica Biológica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Ciudad Universitaria. Instituto de Química y Fisicoquímica Biológica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; Argentina. Fil: Alleva, Karina Edith. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Ciudad Universitaria. Instituto de Química y Fisicoquímica Biológica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática; Argentina 2019-05-03T16:42:26Z 2019-05-03T16:42:26Z 2019-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.14701 http://hdl.handle.net/20.500.12123/5031 1742-464X https://doi.org/10.1111/febs.14701 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley The Febs journal 286 (5) : 991-1002 (Marzo 2019)
spellingShingle Plant Water Relations
Cell Membranes
Relaciones Planta Agua
Membranas Celulares
Aquaporin
Acuaporinas
Vitali, Victoria Andrea
Jozefkowicz, Cintia
Canessa Fortuna, Agustina
Soto, Gabriela Cinthia
Gonzalez Flecha, Francisco Luis
Alleva, Karina Edith
Cooperativity in proton sensing by PIP aquaporins
title Cooperativity in proton sensing by PIP aquaporins
title_full Cooperativity in proton sensing by PIP aquaporins
title_fullStr Cooperativity in proton sensing by PIP aquaporins
title_full_unstemmed Cooperativity in proton sensing by PIP aquaporins
title_short Cooperativity in proton sensing by PIP aquaporins
title_sort cooperativity in proton sensing by pip aquaporins
topic Plant Water Relations
Cell Membranes
Relaciones Planta Agua
Membranas Celulares
Aquaporin
Acuaporinas
url https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.14701
http://hdl.handle.net/20.500.12123/5031
https://doi.org/10.1111/febs.14701
work_keys_str_mv AT vitalivictoriaandrea cooperativityinprotonsensingbypipaquaporins
AT jozefkowiczcintia cooperativityinprotonsensingbypipaquaporins
AT canessafortunaagustina cooperativityinprotonsensingbypipaquaporins
AT sotogabrielacinthia cooperativityinprotonsensingbypipaquaporins
AT gonzalezflechafranciscoluis cooperativityinprotonsensingbypipaquaporins
AT allevakarinaedith cooperativityinprotonsensingbypipaquaporins