Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosp...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
International Journal for Research
2019
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| Subjects: | |
| Online Access: | https://gnpublication.org/index.php/ans/article/view/81/73 http://hdl.handle.net/20.500.12123/5024 |
| _version_ | 1855483540258422784 |
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| author | Reidel, Ivana Gabriela Garcia, Maria Ines González, Verónica Doris Guadalupe Giorello, Antonella Calvinho, Luis Fernando Gennaro, Ana Maria Veaute, Carolina Melania Isabel |
| author_browse | Calvinho, Luis Fernando Garcia, Maria Ines Gennaro, Ana Maria Giorello, Antonella González, Verónica Doris Guadalupe Reidel, Ivana Gabriela Veaute, Carolina Melania Isabel |
| author_facet | Reidel, Ivana Gabriela Garcia, Maria Ines González, Verónica Doris Guadalupe Giorello, Antonella Calvinho, Luis Fernando Gennaro, Ana Maria Veaute, Carolina Melania Isabel |
| author_sort | Reidel, Ivana Gabriela |
| collection | INTA Digital |
| description | The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses. |
| format | Artículo |
| id | INTA5024 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | International Journal for Research |
| publisherStr | International Journal for Research |
| record_format | dspace |
| spelling | INTA50242019-05-03T13:35:42Z Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice Reidel, Ivana Gabriela Garcia, Maria Ines González, Verónica Doris Guadalupe Giorello, Antonella Calvinho, Luis Fernando Gennaro, Ana Maria Veaute, Carolina Melania Isabel Vacuna Respuesta Inmunológica Coadyuvantes Ratón Liposomas (organulos) Vaccines Immune Response Adjuvants Mice Liposomes (organelles) The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses. EEA Rafaela Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina. Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina. Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Giorello, Antonella. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina. Fil: Gennaro, Ana Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina. 2019-05-03T13:33:58Z 2019-05-03T13:33:58Z 2017-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://gnpublication.org/index.php/ans/article/view/81/73 http://hdl.handle.net/20.500.12123/5024 2208-2085 eng info:eu-repo/semantics/restrictedAccess application/pdf International Journal for Research International Journal For Research In Applied And Natural Science 3 (6) : 1-19 (June 2017) |
| spellingShingle | Vacuna Respuesta Inmunológica Coadyuvantes Ratón Liposomas (organulos) Vaccines Immune Response Adjuvants Mice Liposomes (organelles) Reidel, Ivana Gabriela Garcia, Maria Ines González, Verónica Doris Guadalupe Giorello, Antonella Calvinho, Luis Fernando Gennaro, Ana Maria Veaute, Carolina Melania Isabel Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice |
| title | Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice |
| title_full | Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice |
| title_fullStr | Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice |
| title_full_unstemmed | Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice |
| title_short | Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice |
| title_sort | effects of the liposomal co encapsulation of antigen and po cpg oligonucleotide on immune response in mice |
| topic | Vacuna Respuesta Inmunológica Coadyuvantes Ratón Liposomas (organulos) Vaccines Immune Response Adjuvants Mice Liposomes (organelles) |
| url | https://gnpublication.org/index.php/ans/article/view/81/73 http://hdl.handle.net/20.500.12123/5024 |
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