Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their th...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.heliyon.com/article/e01474/ https://www.sciencedirect.com/science/article/pii/S2405844018387383 http://hdl.handle.net/20.500.12123/5020 https://doi.org/10.1016/j.heliyon.2019.e01474 |
| _version_ | 1855483539491913728 |
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| author | Capella, Virginia Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy |
| author_browse | Alustiza, Fabrisio Eduardo Barbero, César A. Bosch, Pablo Capella, Virginia Ibarra, Luis Exequiel Liaudat, Ana Cecilia Mañas, Fernando Rivarola, Claudia R. Rivero, Rebeca E. Rodríguez, Nancy Roma, Dardo Andrés |
| author_facet | Capella, Virginia Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy |
| author_sort | Capella, Virginia |
| collection | INTA Digital |
| description | Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct. |
| format | Artículo |
| id | INTA5020 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA50202022-12-19T16:35:58Z Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel Capella, Virginia Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy Citotoxicidad Biotecnología Células ADN Cytotoxicity Biotechnology Cells DNA Hidrogeles Bioingeniería Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct. EEA Marcos Juárez Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina Fil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-05-02T18:16:52Z 2019-05-02T18:16:52Z 2019-04-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.heliyon.com/article/e01474/ https://www.sciencedirect.com/science/article/pii/S2405844018387383 http://hdl.handle.net/20.500.12123/5020 2405-8440 https://doi.org/10.1016/j.heliyon.2019.e01474 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Heliyon 5 (4) : e01474 (April 2019) |
| spellingShingle | Citotoxicidad Biotecnología Células ADN Cytotoxicity Biotechnology Cells DNA Hidrogeles Bioingeniería Capella, Virginia Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
| title | Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
| title_full | Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
| title_fullStr | Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
| title_full_unstemmed | Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
| title_short | Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
| title_sort | cytotoxicity and bioadhesive properties of poly n isopropylacrylamide hydrogel |
| topic | Citotoxicidad Biotecnología Células ADN Cytotoxicity Biotechnology Cells DNA Hidrogeles Bioingeniería |
| url | https://www.heliyon.com/article/e01474/ https://www.sciencedirect.com/science/article/pii/S2405844018387383 http://hdl.handle.net/20.500.12123/5020 https://doi.org/10.1016/j.heliyon.2019.e01474 |
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