Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults
The control program of codling moth (Cydia pomonella L.) in the Río Negro and Neuquén Valley is intended to neonate larvae. However, adults may be subjected to sublethal pesticide concentrations generating stress which might enhance both mutation rates and activity of the detoxification system. This...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2019
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| Subjects: | |
| Online Access: | https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-7917.12307 http://hdl.handle.net/20.500.12123/5016 https://doi.org/10.1111/1744-7917.12307 |
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| author | Parra Morales, Laura Beatriz Alzogaray, Raúl Adolfo Cichon, Liliana Garrido, Silvina Alejandra Soleño, Jimena Montagna, Cristina Monica |
| author_browse | Alzogaray, Raúl Adolfo Cichon, Liliana Garrido, Silvina Alejandra Montagna, Cristina Monica Parra Morales, Laura Beatriz Soleño, Jimena |
| author_facet | Parra Morales, Laura Beatriz Alzogaray, Raúl Adolfo Cichon, Liliana Garrido, Silvina Alejandra Soleño, Jimena Montagna, Cristina Monica |
| author_sort | Parra Morales, Laura Beatriz |
| collection | INTA Digital |
| description | The control program of codling moth (Cydia pomonella L.) in the Río Negro and Neuquén Valley is intended to neonate larvae. However, adults may be subjected to sublethal pesticide concentrations generating stress which might enhance both mutation rates and activity of the detoxification system. This study assessed the exposure effects of chlorpyrifos on target enzyme and, both detoxifying and antioxidant systems of surviving adults from both a laboratory susceptible strain (LSS) and a field population (FP). The results showed that the FP was as susceptible to chlorpyrifos as the LSS and, both exhibited a similar chlorpyrifos‐inhibitory concentration 50 (IC50) of acetylcholinesterase (AChE). The FP displayed higher carboxylesterase (CarE) and 7‐ethoxycoumarine O‐deethylase (ECOD) activities than LSS. Both LSS and FP showed an increase on CarE activity after the exposure to low‐chlorpyrifos concentrations, followed by enzyme inhibition at higher concentrations. There were no significant differences neither in the activities of glutathione S‐transferases (GST), catalase (CAT) and superoxide dismutase (SOD) nor in the reduced glutathione (GSH) content between LSS and FP. Moreover, these enzymes were unaffected by chlorpyrifos. In conclusion, control adults from the FP exhibited higher CarE and ECOD activities than control adults from the LSS. AChE and CarE activities were the most affected by chlorpyrifos. Control strategies used for C. pomonella, such as rotations of insecticides with different modes of action, will probably delay the evolution of insecticide resistance in FPs from the study area. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5016 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA50162019-04-30T15:37:29Z Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults Parra Morales, Laura Beatriz Alzogaray, Raúl Adolfo Cichon, Liliana Garrido, Silvina Alejandra Soleño, Jimena Montagna, Cristina Monica Lepidoptera Cydia Pomonella Plagas de Plantas Control de Plagas Clorpirifos Pests of Plants Pest Control Chlorpyrifos The control program of codling moth (Cydia pomonella L.) in the Río Negro and Neuquén Valley is intended to neonate larvae. However, adults may be subjected to sublethal pesticide concentrations generating stress which might enhance both mutation rates and activity of the detoxification system. This study assessed the exposure effects of chlorpyrifos on target enzyme and, both detoxifying and antioxidant systems of surviving adults from both a laboratory susceptible strain (LSS) and a field population (FP). The results showed that the FP was as susceptible to chlorpyrifos as the LSS and, both exhibited a similar chlorpyrifos‐inhibitory concentration 50 (IC50) of acetylcholinesterase (AChE). The FP displayed higher carboxylesterase (CarE) and 7‐ethoxycoumarine O‐deethylase (ECOD) activities than LSS. Both LSS and FP showed an increase on CarE activity after the exposure to low‐chlorpyrifos concentrations, followed by enzyme inhibition at higher concentrations. There were no significant differences neither in the activities of glutathione S‐transferases (GST), catalase (CAT) and superoxide dismutase (SOD) nor in the reduced glutathione (GSH) content between LSS and FP. Moreover, these enzymes were unaffected by chlorpyrifos. In conclusion, control adults from the FP exhibited higher CarE and ECOD activities than control adults from the LSS. AChE and CarE activities were the most affected by chlorpyrifos. Control strategies used for C. pomonella, such as rotations of insecticides with different modes of action, will probably delay the evolution of insecticide resistance in FPs from the study area. EEA Alto Valle Fil: Parra Morales, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina Fil: Alzogaray, Raúl Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Cichon, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Garrido, Silvina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Soleño, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina Fil: Montagna, Cristina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina 2019-04-30T15:34:06Z 2019-04-30T15:34:06Z 2017-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-7917.12307 http://hdl.handle.net/20.500.12123/5016 1672-9609 https://doi.org/10.1111/1744-7917.12307 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Insect Science 24 (3) : 455-466 (June 2017) |
| spellingShingle | Lepidoptera Cydia Pomonella Plagas de Plantas Control de Plagas Clorpirifos Pests of Plants Pest Control Chlorpyrifos Parra Morales, Laura Beatriz Alzogaray, Raúl Adolfo Cichon, Liliana Garrido, Silvina Alejandra Soleño, Jimena Montagna, Cristina Monica Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults |
| title | Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults |
| title_full | Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults |
| title_fullStr | Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults |
| title_full_unstemmed | Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults |
| title_short | Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults |
| title_sort | effects of chlorpyrifos on enzymatic systems of cydia pomonella lepidoptera tortricidae adults |
| topic | Lepidoptera Cydia Pomonella Plagas de Plantas Control de Plagas Clorpirifos Pests of Plants Pest Control Chlorpyrifos |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-7917.12307 http://hdl.handle.net/20.500.12123/5016 https://doi.org/10.1111/1744-7917.12307 |
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