A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis

Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are antigenically and genetically similar. The aim of this study was to develop a simple and reliable one-step real time PCR assay with high resolution melting (HRM) analysis for the simultaneous detection and differentiation of BoHV-1 and BoHV-5. O...

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Bibliographic Details
Main Authors: Marin, Maia Solange, Quintana, Silvina, Leunda, Maria Rosa, Recavarren, Mariana Ines, Pagnuco, Inti Anabela, Spath, Ernesto Juan, Perez, Sandra, Odeon, Anselmo Carlos
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2019
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Online Access:https://www.sciencedirect.com/science/article/pii/S0166093415003390
http://hdl.handle.net/20.500.12123/4981
https://doi.org/10.1016/j.jviromet.2015.10.005

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