Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0921448810002221 http://hdl.handle.net/20.500.12123/4945 https://doi.org/10.1016/j.smallrumres.2010.07.021 |
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| author | Manes, Jorgelina Fiorentino, María Andrea Kaiser, German Gustavo Hozbor, Federico Andres Alberio, Ricardo Sánchez, Esteban O. Paolicchi, Fernando |
| author_browse | Alberio, Ricardo Fiorentino, María Andrea Hozbor, Federico Andres Kaiser, German Gustavo Manes, Jorgelina Paolicchi, Fernando Sánchez, Esteban O. |
| author_facet | Manes, Jorgelina Fiorentino, María Andrea Kaiser, German Gustavo Hozbor, Federico Andres Alberio, Ricardo Sánchez, Esteban O. Paolicchi, Fernando |
| author_sort | Manes, Jorgelina |
| collection | INTA Digital |
| description | The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14 days). Twenty-one multiparous Texel ewes were randomly allocated to 4 treatment groups during the breeding season. In the ewes from Group I (n = 6) and Group II (n = 5), a polyurethane sponge containing 60 mg MAP was inserted in the vagina for a period of 7 or 14 days, respectively. In the ewes of Group III (n = 5) and Group IV (n = 5), an intravaginal progesterone releasing insert (IVP4) containing 160 mg of progesterone in an inert silicone device, was inserted for 7 or 14 days, respectively. At device withdrawal, each ewe was treated with 200 IU eCG i.m. Standard bacteriological procedures were performed on vaginal mucus samples obtained before the introduction of the devices, at device withdrawal and on the day of AI in all groups. Estrus was recorded with the aid of vasectomized rams every 12 h, and AI was performed 52–54 h after device withdrawal, using fresh semen. The intervals between device withdrawal and estrus were: Group I: 56.4 ± 21.5 h; Group II: 42.0 ± 33.9 h; Group III: 51.6 ± 21.5 h; Group IV: 37.2 ± 10.7 h, while the pregnancy rates were: Group I: 83.3%; Group II: 60.0%; Group III: 60.0%; Group IV: 60.0%. The pregnancy rates and the interval between device withdrawal and the occurrence of estrus did not differ between treatments. The predominant bacterial flora population at device insertion was mostly gram positive (G+) (90%) bacteria. The strains most frequently found were Bacilllus sp., Staphylococcus sp. and Corynebacterium sp. Of the 19 isolates made at device removal, 79% were gram negative, with the Escherichia sp. being the most frequently isolated. At the time of AI and regardless of the device used, the 14-day treatment group presented an initial gram positive bacterial flora, while the 7-day groups presented gram negative flora (82%). It could be concluded that the use of intravaginal devices, regardless of their composition (silicone or polyurethane), may generate changes in the normal vaginal bacterial flora of the vaginal mucus. These changes did not reflect on the subsequent fertility. The use of intravaginal devices should however include the adoption of strict hygiene procedures, to minimize the growth of bacterial flora. |
| format | Artículo |
| id | INTA4945 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA49452019-04-22T15:23:32Z Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes Manes, Jorgelina Fiorentino, María Andrea Kaiser, German Gustavo Hozbor, Federico Andres Alberio, Ricardo Sánchez, Esteban O. Paolicchi, Fernando Oveja Flora Microbiana Ciclo Estral Bacteria Ewes Microbial Flora Oestrous Cycle Microbiota Vaginal Dispositivos Intravaginales The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14 days). Twenty-one multiparous Texel ewes were randomly allocated to 4 treatment groups during the breeding season. In the ewes from Group I (n = 6) and Group II (n = 5), a polyurethane sponge containing 60 mg MAP was inserted in the vagina for a period of 7 or 14 days, respectively. In the ewes of Group III (n = 5) and Group IV (n = 5), an intravaginal progesterone releasing insert (IVP4) containing 160 mg of progesterone in an inert silicone device, was inserted for 7 or 14 days, respectively. At device withdrawal, each ewe was treated with 200 IU eCG i.m. Standard bacteriological procedures were performed on vaginal mucus samples obtained before the introduction of the devices, at device withdrawal and on the day of AI in all groups. Estrus was recorded with the aid of vasectomized rams every 12 h, and AI was performed 52–54 h after device withdrawal, using fresh semen. The intervals between device withdrawal and estrus were: Group I: 56.4 ± 21.5 h; Group II: 42.0 ± 33.9 h; Group III: 51.6 ± 21.5 h; Group IV: 37.2 ± 10.7 h, while the pregnancy rates were: Group I: 83.3%; Group II: 60.0%; Group III: 60.0%; Group IV: 60.0%. The pregnancy rates and the interval between device withdrawal and the occurrence of estrus did not differ between treatments. The predominant bacterial flora population at device insertion was mostly gram positive (G+) (90%) bacteria. The strains most frequently found were Bacilllus sp., Staphylococcus sp. and Corynebacterium sp. Of the 19 isolates made at device removal, 79% were gram negative, with the Escherichia sp. being the most frequently isolated. At the time of AI and regardless of the device used, the 14-day treatment group presented an initial gram positive bacterial flora, while the 7-day groups presented gram negative flora (82%). It could be concluded that the use of intravaginal devices, regardless of their composition (silicone or polyurethane), may generate changes in the normal vaginal bacterial flora of the vaginal mucus. These changes did not reflect on the subsequent fertility. The use of intravaginal devices should however include the adoption of strict hygiene procedures, to minimize the growth of bacterial flora. EEA Balcarce Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: Sánchez, Esteban O. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina 2019-04-22T15:20:24Z 2019-04-22T15:20:24Z 2010-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0921448810002221 http://hdl.handle.net/20.500.12123/4945 0921-4488 https://doi.org/10.1016/j.smallrumres.2010.07.021 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Small Ruminant Research 94 (1–3) : 201-204. (November 2010) |
| spellingShingle | Oveja Flora Microbiana Ciclo Estral Bacteria Ewes Microbial Flora Oestrous Cycle Microbiota Vaginal Dispositivos Intravaginales Manes, Jorgelina Fiorentino, María Andrea Kaiser, German Gustavo Hozbor, Federico Andres Alberio, Ricardo Sánchez, Esteban O. Paolicchi, Fernando Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| title | Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| title_full | Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| title_fullStr | Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| title_full_unstemmed | Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| title_short | Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| title_sort | changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes |
| topic | Oveja Flora Microbiana Ciclo Estral Bacteria Ewes Microbial Flora Oestrous Cycle Microbiota Vaginal Dispositivos Intravaginales |
| url | https://www.sciencedirect.com/science/article/pii/S0921448810002221 http://hdl.handle.net/20.500.12123/4945 https://doi.org/10.1016/j.smallrumres.2010.07.021 |
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