Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7
Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the c...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422 http://hdl.handle.net/20.500.12123/491 https://doi.org/10.1371/journal.pone.0169422 |
| _version_ | 1855034658174009344 |
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| author | Martorelli, Luisina Garbaccio, Sergio Gabriel Vilte, Daniel Alejandro Albanese, Adriana Andrea Mejías, María Pilar Palermo, Marina Sandra Mercado, Elsa Cristina Ibarra, Cristina E. Cataldi, Angel Adrian |
| author_browse | Albanese, Adriana Andrea Cataldi, Angel Adrian Garbaccio, Sergio Gabriel Ibarra, Cristina E. Martorelli, Luisina Mejías, María Pilar Mercado, Elsa Cristina Palermo, Marina Sandra Vilte, Daniel Alejandro |
| author_facet | Martorelli, Luisina Garbaccio, Sergio Gabriel Vilte, Daniel Alejandro Albanese, Adriana Andrea Mejías, María Pilar Palermo, Marina Sandra Mercado, Elsa Cristina Ibarra, Cristina E. Cataldi, Angel Adrian |
| author_sort | Martorelli, Luisina |
| collection | INTA Digital |
| description | Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group. All antigens were expressed as recombinant proteins in E. coli. Specific IgG titres increased in vaccinated calves and the inclusion of BLS-Stx2B in the formulation seems to have a stimulatory effect on the humoral response to IntiminC280 and EspB after the booster. The neutralizing activity of antibodies against these two antigens was assessed in Red Blood Cell lysis assays and adherence to Hep-2 cells as a correlate of T3SS activity. Both sera from animals vaccinated with 2 or 3 antigens inhibited both virulence properties. Serological response to Stx2 was observed in animals vaccinated only with BLS-Stx2B and with 3 antigens and neutralization of Stx2 cytotoxicity was also observed in both groups. In conclusion, immunization of calves with BLS-Stx2B, IntiminC280 and EspB elicited a potent humoral response able to neutralize Shiga toxin 2 cytotoxity and the T3SS virulence properties in vitro. These results suggest that this formulation is a good candidate vaccine to reduce STEC shedding in cattle and needs to be further assessed in vivo |
| format | info:ar-repo/semantics/artículo |
| id | INTA491 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA4912019-01-23T11:51:52Z Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 Martorelli, Luisina Garbaccio, Sergio Gabriel Vilte, Daniel Alejandro Albanese, Adriana Andrea Mejías, María Pilar Palermo, Marina Sandra Mercado, Elsa Cristina Ibarra, Cristina E. Cataldi, Angel Adrian Ternero Respuesta Inmunológica Vacunación Antígenos Bacterianos Escherichia Coli Calves Immune Response Vaccination Bacterial Antigens Toxina Shiga Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group. All antigens were expressed as recombinant proteins in E. coli. Specific IgG titres increased in vaccinated calves and the inclusion of BLS-Stx2B in the formulation seems to have a stimulatory effect on the humoral response to IntiminC280 and EspB after the booster. The neutralizing activity of antibodies against these two antigens was assessed in Red Blood Cell lysis assays and adherence to Hep-2 cells as a correlate of T3SS activity. Both sera from animals vaccinated with 2 or 3 antigens inhibited both virulence properties. Serological response to Stx2 was observed in animals vaccinated only with BLS-Stx2B and with 3 antigens and neutralization of Stx2 cytotoxicity was also observed in both groups. In conclusion, immunization of calves with BLS-Stx2B, IntiminC280 and EspB elicited a potent humoral response able to neutralize Shiga toxin 2 cytotoxity and the T3SS virulence properties in vitro. These results suggest that this formulation is a good candidate vaccine to reduce STEC shedding in cattle and needs to be further assessed in vivo Inst. de Patobiología Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Albanese, Adriana Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Mejias, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos; Argentina Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Ibarra, Cristina E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-06-28T13:23:54Z 2017-06-28T13:23:54Z 2017-01-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422 http://hdl.handle.net/20.500.12123/491 https://doi.org/10.1371/journal.pone.0169422 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLoS One 12(1) : 2017 |
| spellingShingle | Ternero Respuesta Inmunológica Vacunación Antígenos Bacterianos Escherichia Coli Calves Immune Response Vaccination Bacterial Antigens Toxina Shiga Martorelli, Luisina Garbaccio, Sergio Gabriel Vilte, Daniel Alejandro Albanese, Adriana Andrea Mejías, María Pilar Palermo, Marina Sandra Mercado, Elsa Cristina Ibarra, Cristina E. Cataldi, Angel Adrian Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
| title | Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
| title_full | Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
| title_fullStr | Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
| title_full_unstemmed | Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
| title_short | Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
| title_sort | immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2b subunit of escherichia coli o157 h7 |
| topic | Ternero Respuesta Inmunológica Vacunación Antígenos Bacterianos Escherichia Coli Calves Immune Response Vaccination Bacterial Antigens Toxina Shiga |
| url | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422 http://hdl.handle.net/20.500.12123/491 https://doi.org/10.1371/journal.pone.0169422 |
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