Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins
Bovine gammaherpesvirus 4 (BoHV4) is a member of the family Herpesviridae. In Argentina, BoHV4 was isolated and characterized in 2007 from samples of aborted cows. Argentinean isolates are highly divergent and are classified as: Genotype 1(Movar-like), Genotype 2 (DN599-like) and Genotype 3 (a novel...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0147957119300189 http://hdl.handle.net/20.500.12123/4904 https://doi.org/10.1016/j.cimid.2019.01.014 |
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| author | Morán, Pedro Pérez, Sandra Odeon, Anselmo Carlos Verna, Andrea Elizabeth |
| author_browse | Morán, Pedro Odeon, Anselmo Carlos Pérez, Sandra Verna, Andrea Elizabeth |
| author_facet | Morán, Pedro Pérez, Sandra Odeon, Anselmo Carlos Verna, Andrea Elizabeth |
| author_sort | Morán, Pedro |
| collection | INTA Digital |
| description | Bovine gammaherpesvirus 4 (BoHV4) is a member of the family Herpesviridae. In Argentina, BoHV4 was isolated and characterized in 2007 from samples of aborted cows. Argentinean isolates are highly divergent and are classified as: Genotype 1(Movar-like), Genotype 2 (DN599-like) and Genotype 3 (a novel group). The aim of this study was to comparatively evaluate the biological characteristics of six Argentinean BoHV4 field isolates in cell lines from different origins. All strains induced productive infection in the cell lines used, with different degrees of permissiveness. A direct relationship among the times of appearance of cytopathic effect, the growth kinetics, the size of the lysis plaques and the virulent-like behaviour in vitro could not be established. However, although slight, there are differences in the biological behaviour of the BoHV4 fields isolates analyzed. This variability is independent of their genetic classification but would be conditioned by the nature of the infected cells. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4904 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA49042019-04-15T14:08:19Z Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins Morán, Pedro Pérez, Sandra Odeon, Anselmo Carlos Verna, Andrea Elizabeth Ganado Bovino Herpesviridae Herpes Virus Bovino Enfermedades de los Animales Replicación Aislamiento Campo Argentina Cattle Bovine Herpesvirus Replication Isolation Fields Animal Diseases Bovine gammaherpesvirus 4 (BoHV4) is a member of the family Herpesviridae. In Argentina, BoHV4 was isolated and characterized in 2007 from samples of aborted cows. Argentinean isolates are highly divergent and are classified as: Genotype 1(Movar-like), Genotype 2 (DN599-like) and Genotype 3 (a novel group). The aim of this study was to comparatively evaluate the biological characteristics of six Argentinean BoHV4 field isolates in cell lines from different origins. All strains induced productive infection in the cell lines used, with different degrees of permissiveness. A direct relationship among the times of appearance of cytopathic effect, the growth kinetics, the size of the lysis plaques and the virulent-like behaviour in vitro could not be established. However, although slight, there are differences in the biological behaviour of the BoHV4 fields isolates analyzed. This variability is independent of their genetic classification but would be conditioned by the nature of the infected cells. EEA Balcarce Fil: Morán, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Odeón, Anselmo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Verna, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2019-04-15T13:57:57Z 2019-04-15T13:57:57Z 2019-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0147957119300189 http://hdl.handle.net/20.500.12123/4904 0147-9571 https://doi.org/10.1016/j.cimid.2019.01.014 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 63 : 97-103 ( 2019) |
| spellingShingle | Ganado Bovino Herpesviridae Herpes Virus Bovino Enfermedades de los Animales Replicación Aislamiento Campo Argentina Cattle Bovine Herpesvirus Replication Isolation Fields Animal Diseases Morán, Pedro Pérez, Sandra Odeon, Anselmo Carlos Verna, Andrea Elizabeth Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins |
| title | Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins |
| title_full | Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins |
| title_fullStr | Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins |
| title_full_unstemmed | Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins |
| title_short | Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins |
| title_sort | comparative analysis of replicative properties of phylogenetically divergent argentinean bohv 4 strains in cell lines from different origins |
| topic | Ganado Bovino Herpesviridae Herpes Virus Bovino Enfermedades de los Animales Replicación Aislamiento Campo Argentina Cattle Bovine Herpesvirus Replication Isolation Fields Animal Diseases |
| url | https://www.sciencedirect.com/science/article/pii/S0147957119300189 http://hdl.handle.net/20.500.12123/4904 https://doi.org/10.1016/j.cimid.2019.01.014 |
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