Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos
Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Plos One
2019
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| Subjects: | |
| Online Access: | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108139 http://hdl.handle.net/20.500.12123/4880 https://doi.org/10.1371/journal.pone.0108139 |
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| author | Cánepa, María Jesús Ortega, Nicolás Matías Monteleone, Melisa Carolina Mucci, Nicolas Crescencio Kaiser, German Gustavo Brocco, Marcela Adriana Mutto, Adrián Angel |
| author_browse | Brocco, Marcela Adriana Cánepa, María Jesús Kaiser, German Gustavo Monteleone, Melisa Carolina Mucci, Nicolas Crescencio Mutto, Adrián Angel Ortega, Nicolás Matías |
| author_facet | Cánepa, María Jesús Ortega, Nicolás Matías Monteleone, Melisa Carolina Mucci, Nicolas Crescencio Kaiser, German Gustavo Brocco, Marcela Adriana Mutto, Adrián Angel |
| author_sort | Cánepa, María Jesús |
| collection | INTA Digital |
| description | Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both for commercial purposes and for scientific research. Previous works have demonstrated that in vitro culture systems induce alterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this work was to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum (ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunit β5, PSMB5) and apoptosis (BCL-2 associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT and compare it with that of their in vivo counterparts. Poly (A) + mRNA was isolated from three pools of 10 blastocysts per treatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) was significantly increased in SCNT embryos as compared with that of in vivo-derived blastocysts. No significant differences were found in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analyses in the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improve the efficiency of assisted reproductive techniques (ART). |
| format | info:ar-repo/semantics/artículo |
| id | INTA4880 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Plos One |
| publisherStr | Plos One |
| record_format | dspace |
| spelling | INTA48802019-04-11T13:25:33Z Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos Cánepa, María Jesús Ortega, Nicolás Matías Monteleone, Melisa Carolina Mucci, Nicolas Crescencio Kaiser, German Gustavo Brocco, Marcela Adriana Mutto, Adrián Angel Biotecnología Reproducción Biotecnología Animal Diferenciación Celular Transferencia de Embriones Ganado Bovino Fecundación in Vitro Genes Biotechnology Reproduction Animal Biotechnology Cell Differentiation Embryo Transfer Cattle In Vitro Fertilization Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both for commercial purposes and for scientific research. Previous works have demonstrated that in vitro culture systems induce alterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this work was to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum (ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunit β5, PSMB5) and apoptosis (BCL-2 associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT and compare it with that of their in vivo counterparts. Poly (A) + mRNA was isolated from three pools of 10 blastocysts per treatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) was significantly increased in SCNT embryos as compared with that of in vivo-derived blastocysts. No significant differences were found in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analyses in the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improve the efficiency of assisted reproductive techniques (ART). EEA Balcarce Fil: Cánepa, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Ortega, Nicolás Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Monteleone, Melisa Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Mucci, Nicolas Crescencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproducción; Argentina Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproducción; Argentina Fil: Brocco, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina 2019-04-11T13:24:19Z 2019-04-11T13:24:19Z 2014-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108139 http://hdl.handle.net/20.500.12123/4880 1932-6203 https://doi.org/10.1371/journal.pone.0108139 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos One PLoS ONE 9 (9) : e108139 (September 2014) |
| spellingShingle | Biotecnología Reproducción Biotecnología Animal Diferenciación Celular Transferencia de Embriones Ganado Bovino Fecundación in Vitro Genes Biotechnology Reproduction Animal Biotechnology Cell Differentiation Embryo Transfer Cattle In Vitro Fertilization Cánepa, María Jesús Ortega, Nicolás Matías Monteleone, Melisa Carolina Mucci, Nicolas Crescencio Kaiser, German Gustavo Brocco, Marcela Adriana Mutto, Adrián Angel Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| title | Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| title_full | Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| title_fullStr | Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| title_full_unstemmed | Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| title_short | Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| title_sort | expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos |
| topic | Biotecnología Reproducción Biotecnología Animal Diferenciación Celular Transferencia de Embriones Ganado Bovino Fecundación in Vitro Genes Biotechnology Reproduction Animal Biotechnology Cell Differentiation Embryo Transfer Cattle In Vitro Fertilization |
| url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108139 http://hdl.handle.net/20.500.12123/4880 https://doi.org/10.1371/journal.pone.0108139 |
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