Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening
Excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and m...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
American Chemical Society
2019
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| Materias: | |
| Acceso en línea: | https://pubs.acs.org/doi/abs/10.1021/jf504357u http://hdl.handle.net/20.500.12123/4874 https://dx.doi.org/10.1021/jf504357u |
| _version_ | 1855035399328497664 |
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| author | Basanta, Maria Florencia Ponce, Nora Marta Andrea Salum, Maria Laura Raffo Benegas, Maria Dolores Vicente, Ariel Roberto Erra Balsells, Rosa Stortz, Carlos Arturo |
| author_browse | Basanta, Maria Florencia Erra Balsells, Rosa Ponce, Nora Marta Andrea Raffo Benegas, Maria Dolores Salum, Maria Laura Stortz, Carlos Arturo Vicente, Ariel Roberto |
| author_facet | Basanta, Maria Florencia Ponce, Nora Marta Andrea Salum, Maria Laura Raffo Benegas, Maria Dolores Vicente, Ariel Roberto Erra Balsells, Rosa Stortz, Carlos Arturo |
| author_sort | Basanta, Maria Florencia |
| collection | INTA Digital |
| description | Excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and monosaccharide composition of pectin and hemicelluloses from five sweet cherry cultivars (‘Chelan’, ‘Sumele’, ‘Brooks’, ‘Sunburst’, and ‘Regina’) with contrasting firmness and cracking susceptibility at two developmental stages (immature and ripe). In contrast to what is usually shown in most fruits, cherry softening could occur is some cultivars without marked increases in water-soluble pectin. Although polyuronide and hemicellulose depolymerization was observed in the water-soluble and dilute-alkali-soluble fractions, only moderate association occurs between initial polymer size and cultivar firmness. In all the genotypes the Na2CO3-soluble polysaccharides (NSF) represented the most abundant and dynamic wall fraction during ripening. Firm cultivars showed upon ripening a lower neutral sugars/uronic acid ratio in the NSF, suggesting that they have a lower proportion of highly branched polyuronides. The similar molar ratios of arabinose plus galactose to rhamnose [(Ara+Gal)/Rha] suggest that the cultivars differed in their relative proportion of homogalacturonan (HG) and rhamnogalacturonan I (RG-I) rather than in the size of the RG side chains; with greater proportions of HG in firmer cherries. Ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was useful to identify the depolymerization patterns of weakly bound pectins, but gave less accurate results on ionically bound pectins, and was unable to find any pattern on covalently bound pectins. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4874 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | INTA48742019-04-11T11:27:27Z Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening Basanta, Maria Florencia Ponce, Nora Marta Andrea Salum, Maria Laura Raffo Benegas, Maria Dolores Vicente, Ariel Roberto Erra Balsells, Rosa Stortz, Carlos Arturo Cereza Variedades Pared Celular Polisacáridos Maduramiento Genotipos Cherries Varieties Cell Walls Polysaccharides Ripening Genotypes Prunus Avium Excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and monosaccharide composition of pectin and hemicelluloses from five sweet cherry cultivars (‘Chelan’, ‘Sumele’, ‘Brooks’, ‘Sunburst’, and ‘Regina’) with contrasting firmness and cracking susceptibility at two developmental stages (immature and ripe). In contrast to what is usually shown in most fruits, cherry softening could occur is some cultivars without marked increases in water-soluble pectin. Although polyuronide and hemicellulose depolymerization was observed in the water-soluble and dilute-alkali-soluble fractions, only moderate association occurs between initial polymer size and cultivar firmness. In all the genotypes the Na2CO3-soluble polysaccharides (NSF) represented the most abundant and dynamic wall fraction during ripening. Firm cultivars showed upon ripening a lower neutral sugars/uronic acid ratio in the NSF, suggesting that they have a lower proportion of highly branched polyuronides. The similar molar ratios of arabinose plus galactose to rhamnose [(Ara+Gal)/Rha] suggest that the cultivars differed in their relative proportion of homogalacturonan (HG) and rhamnogalacturonan I (RG-I) rather than in the size of the RG side chains; with greater proportions of HG in firmer cherries. Ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was useful to identify the depolymerization patterns of weakly bound pectins, but gave less accurate results on ionically bound pectins, and was unable to find any pattern on covalently bound pectins. EEA Alto Valle Fil: Basanta, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Ponce, Nora Marta Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Salum, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Raffo Benegas, Maria Dolores. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Vicente, Ariel Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Agroindustrias; Argentina Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Stortz, Carlos Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina 2019-04-11T11:24:56Z 2019-04-11T11:24:56Z 2014-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://pubs.acs.org/doi/abs/10.1021/jf504357u http://hdl.handle.net/20.500.12123/4874 0021-8561 1520-5118 https://dx.doi.org/10.1021/jf504357u eng info:eu-repo/semantics/restrictedAccess application/pdf American Chemical Society Journal of Agricultural and Food Chemistry 62 (51) : 12418–12427 (December 2014) |
| spellingShingle | Cereza Variedades Pared Celular Polisacáridos Maduramiento Genotipos Cherries Varieties Cell Walls Polysaccharides Ripening Genotypes Prunus Avium Basanta, Maria Florencia Ponce, Nora Marta Andrea Salum, Maria Laura Raffo Benegas, Maria Dolores Vicente, Ariel Roberto Erra Balsells, Rosa Stortz, Carlos Arturo Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening |
| title | Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening |
| title_full | Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening |
| title_fullStr | Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening |
| title_full_unstemmed | Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening |
| title_short | Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening |
| title_sort | compositional changes in cell wall polysaccharides from five sweet cherry prunus avium l cultivars during on tree ripening |
| topic | Cereza Variedades Pared Celular Polisacáridos Maduramiento Genotipos Cherries Varieties Cell Walls Polysaccharides Ripening Genotypes Prunus Avium |
| url | https://pubs.acs.org/doi/abs/10.1021/jf504357u http://hdl.handle.net/20.500.12123/4874 https://dx.doi.org/10.1021/jf504357u |
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