25S–18S rDNA IGS of Capsicum: molecular structure and comparison
The primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The recognized functional IGS is 2,078 bp in length. According to nucleotide base...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2019
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| Online Access: | https://link.springer.com/article/10.1007/s00606-011-0546-8 http://hdl.handle.net/20.500.12123/4859 https://doi.org/10.1007/s00606-011-0546-8 |
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| author | Grabiele, Mauro Debat, Humberto Julio Moscone, Eduardo Alberto Ducasse, Daniel Adrian |
| author_browse | Debat, Humberto Julio Ducasse, Daniel Adrian Grabiele, Mauro Moscone, Eduardo Alberto |
| author_facet | Grabiele, Mauro Debat, Humberto Julio Moscone, Eduardo Alberto Ducasse, Daniel Adrian |
| author_sort | Grabiele, Mauro |
| collection | INTA Digital |
| description | The primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The recognized functional IGS is 2,078 bp in length. According to nucleotide base composition, regulatory elements, and conserved and repeated sequences the IGS can be divided into seven structural regions (SRI–VII). SRI comprises three copies of GAGGTTTTT-like motif, a probable transcription termination site in Solanaceae. At 3′-end, there are 21 bp matching the 18S rDNA. SRII is formed by 47 repeats of CACCATGG-like motif, the shortest repetitive region found in plant rDNA to date. SRIII is highly AT-rich, preceding SRIV, a highly conserved region in Solanaceae containing the transcription initiation site (TIS) TATATAAGGGGGG. The external transcribed spacer (ETS) is 966 bp in length. SRV-VII, downstream of the TIS, possesses eight inverted repeats, and three predicted stem-loops show pre-micro RNA (miRNA)-like structural features. Intragenomic variation is presented, and data are compared with characterized Solanaceae 25S–18S rDNA IGS. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4859 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA48592019-04-09T14:27:16Z 25S–18S rDNA IGS of Capsicum: molecular structure and comparison Grabiele, Mauro Debat, Humberto Julio Moscone, Eduardo Alberto Ducasse, Daniel Adrian Capsicum Genética ADN ADN Intergénico Marcadores Genéticos Genetics DNA Intergenic DNA Genetic Markers Marcadores Moleculares The primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The recognized functional IGS is 2,078 bp in length. According to nucleotide base composition, regulatory elements, and conserved and repeated sequences the IGS can be divided into seven structural regions (SRI–VII). SRI comprises three copies of GAGGTTTTT-like motif, a probable transcription termination site in Solanaceae. At 3′-end, there are 21 bp matching the 18S rDNA. SRII is formed by 47 repeats of CACCATGG-like motif, the shortest repetitive region found in plant rDNA to date. SRIII is highly AT-rich, preceding SRIV, a highly conserved region in Solanaceae containing the transcription initiation site (TIS) TATATAAGGGGGG. The external transcribed spacer (ETS) is 966 bp in length. SRV-VII, downstream of the TIS, possesses eight inverted repeats, and three predicted stem-loops show pre-micro RNA (miRNA)-like structural features. Intragenomic variation is presented, and data are compared with characterized Solanaceae 25S–18S rDNA IGS. Instituto de Patología Vegetal Fil: Grabiele, Mauro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2019-04-09T14:26:00Z 2019-04-09T14:26:00Z 2012-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00606-011-0546-8 http://hdl.handle.net/20.500.12123/4859 0378-2697 1615-6110 https://doi.org/10.1007/s00606-011-0546-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Systematics and Evolution 298 (2) : 313–321 (February 2012) |
| spellingShingle | Capsicum Genética ADN ADN Intergénico Marcadores Genéticos Genetics DNA Intergenic DNA Genetic Markers Marcadores Moleculares Grabiele, Mauro Debat, Humberto Julio Moscone, Eduardo Alberto Ducasse, Daniel Adrian 25S–18S rDNA IGS of Capsicum: molecular structure and comparison |
| title | 25S–18S rDNA IGS of Capsicum: molecular structure and comparison |
| title_full | 25S–18S rDNA IGS of Capsicum: molecular structure and comparison |
| title_fullStr | 25S–18S rDNA IGS of Capsicum: molecular structure and comparison |
| title_full_unstemmed | 25S–18S rDNA IGS of Capsicum: molecular structure and comparison |
| title_short | 25S–18S rDNA IGS of Capsicum: molecular structure and comparison |
| title_sort | 25s 18s rdna igs of capsicum molecular structure and comparison |
| topic | Capsicum Genética ADN ADN Intergénico Marcadores Genéticos Genetics DNA Intergenic DNA Genetic Markers Marcadores Moleculares |
| url | https://link.springer.com/article/10.1007/s00606-011-0546-8 http://hdl.handle.net/20.500.12123/4859 https://doi.org/10.1007/s00606-011-0546-8 |
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