Experimental selection of long‐term intracellular mycobacteria
Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure....
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4858 https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section https://doi.org/10.1111/cmi.12303 |
| _version_ | 1855483510639296512 |
|---|---|
| author | Vazquez, Cristina Lourdes Lerner, Thomas R. Kasmapour, Bahram Pei, Gang Gronow, Achim Bianco, María Veronica Blanco, Federico Carlos Bleck, Christopher K.E. Geffers, Robert Bigi, Fabiana Abraham, Wolf-Rainer Gutierrez, Maximiliano Gabriel |
| author_browse | Abraham, Wolf-Rainer Bianco, María Veronica Bigi, Fabiana Blanco, Federico Carlos Bleck, Christopher K.E. Geffers, Robert Gronow, Achim Gutierrez, Maximiliano Gabriel Kasmapour, Bahram Lerner, Thomas R. Pei, Gang Vazquez, Cristina Lourdes |
| author_facet | Vazquez, Cristina Lourdes Lerner, Thomas R. Kasmapour, Bahram Pei, Gang Gronow, Achim Bianco, María Veronica Blanco, Federico Carlos Bleck, Christopher K.E. Geffers, Robert Bigi, Fabiana Abraham, Wolf-Rainer Gutierrez, Maximiliano Gabriel |
| author_sort | Vazquez, Cristina Lourdes |
| collection | INTA Digital |
| description | Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments. |
| format | Artículo |
| id | INTA4858 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA48582019-04-09T14:31:50Z Experimental selection of long‐term intracellular mycobacteria Vazquez, Cristina Lourdes Lerner, Thomas R. Kasmapour, Bahram Pei, Gang Gronow, Achim Bianco, María Veronica Blanco, Federico Carlos Bleck, Christopher K.E. Geffers, Robert Bigi, Fabiana Abraham, Wolf-Rainer Gutierrez, Maximiliano Gabriel Mycobacterium Experimentos de Largo Plazo Líquido Intracellular Mycobacterium Bovis Glicolípidos Supervivencia Long Term Experiments Intracellular Fluid Glycolipids Survival Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments. Instituto de Biotecnología Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania Fil: Lerner, Thomas R. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña Fil: Kasmapour, Bahram. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania Fil: Pei, Gang. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania Fil: Gronow, Achim. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Bleck, Christopher K.E. University of Basel. Centre for Cellular Imaging and NanoAnalytics, Structural Biology and Biophysics, Biozentrum, Mattenstrasse 26; Suiza Fil: Geffers, Robert. Helmholtz Centre for Infection Research. Research Group Genome Analytics; Alemania Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Abraham, Wolf-Rainer. Helmholtz Centre for Infection Research. Research Group Chemical Microbiology; Alemania Fil: Gutierrez, Maximiliano Gabriel. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña 2019-04-09T14:25:13Z 2019-04-09T14:25:13Z 2014-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4858 https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section 1462-5814 https://doi.org/10.1111/cmi.12303 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Cellular microbiology 16 (9) : 1425-1440. (September 2014) |
| spellingShingle | Mycobacterium Experimentos de Largo Plazo Líquido Intracellular Mycobacterium Bovis Glicolípidos Supervivencia Long Term Experiments Intracellular Fluid Glycolipids Survival Vazquez, Cristina Lourdes Lerner, Thomas R. Kasmapour, Bahram Pei, Gang Gronow, Achim Bianco, María Veronica Blanco, Federico Carlos Bleck, Christopher K.E. Geffers, Robert Bigi, Fabiana Abraham, Wolf-Rainer Gutierrez, Maximiliano Gabriel Experimental selection of long‐term intracellular mycobacteria |
| title | Experimental selection of long‐term intracellular mycobacteria |
| title_full | Experimental selection of long‐term intracellular mycobacteria |
| title_fullStr | Experimental selection of long‐term intracellular mycobacteria |
| title_full_unstemmed | Experimental selection of long‐term intracellular mycobacteria |
| title_short | Experimental selection of long‐term intracellular mycobacteria |
| title_sort | experimental selection of long term intracellular mycobacteria |
| topic | Mycobacterium Experimentos de Largo Plazo Líquido Intracellular Mycobacterium Bovis Glicolípidos Supervivencia Long Term Experiments Intracellular Fluid Glycolipids Survival |
| url | http://hdl.handle.net/20.500.12123/4858 https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section https://doi.org/10.1111/cmi.12303 |
| work_keys_str_mv | AT vazquezcristinalourdes experimentalselectionoflongtermintracellularmycobacteria AT lernerthomasr experimentalselectionoflongtermintracellularmycobacteria AT kasmapourbahram experimentalselectionoflongtermintracellularmycobacteria AT peigang experimentalselectionoflongtermintracellularmycobacteria AT gronowachim experimentalselectionoflongtermintracellularmycobacteria AT biancomariaveronica experimentalselectionoflongtermintracellularmycobacteria AT blancofedericocarlos experimentalselectionoflongtermintracellularmycobacteria AT bleckchristopherke experimentalselectionoflongtermintracellularmycobacteria AT geffersrobert experimentalselectionoflongtermintracellularmycobacteria AT bigifabiana experimentalselectionoflongtermintracellularmycobacteria AT abrahamwolfrainer experimentalselectionoflongtermintracellularmycobacteria AT gutierrezmaximilianogabriel experimentalselectionoflongtermintracellularmycobacteria |