Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars

Background: Diversity in crops is fundamental for plant breeding efforts. An accurate assessment of genetic diversity, using molecular markers, such as single nucleotide polymorphism (SNP), must be able to reveal the structure of the population under study. A characterization of population structure...

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Autores principales: Tagliotti, Martín, Deperi, Sofia, Bedogni, Maria Cecilia, Zhang, Roufang, Manrique Carpintero, Norma C., Coombs, Joseph, Douches, David, Huarte, Marcelo Atilio
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer Nature 2019
Materias:
Acceso en línea:https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-017-0556-9
http://hdl.handle.net/20.500.12123/4849
https://doi.org/10.1186/s12863-017-0556-9
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author Tagliotti, Martín
Deperi, Sofia
Bedogni, Maria Cecilia
Zhang, Roufang
Manrique Carpintero, Norma C.
Coombs, Joseph
Douches, David
Huarte, Marcelo Atilio
author_browse Bedogni, Maria Cecilia
Coombs, Joseph
Deperi, Sofia
Douches, David
Huarte, Marcelo Atilio
Manrique Carpintero, Norma C.
Tagliotti, Martín
Zhang, Roufang
author_facet Tagliotti, Martín
Deperi, Sofia
Bedogni, Maria Cecilia
Zhang, Roufang
Manrique Carpintero, Norma C.
Coombs, Joseph
Douches, David
Huarte, Marcelo Atilio
author_sort Tagliotti, Martín
collection INTA Digital
description Background: Diversity in crops is fundamental for plant breeding efforts. An accurate assessment of genetic diversity, using molecular markers, such as single nucleotide polymorphism (SNP), must be able to reveal the structure of the population under study. A characterization of population structure using easy measurable phenotypic traits could be a preliminary and low-cost approach to elucidate the genetic structure of a population. A potato population of 183 genotypes was evaluated using 4859 high-quality SNPs and 19 phenotypic traits commonly recorded in potato breeding programs. A Bayesian approach, Minimum Spanning Tree (MST) and diversity estimator, as well as multivariate analysis based on phenotypic traits, were adopted to assess the population structure. Results: Analysis based on molecular markers showed groups linked to the phylogenetic relationship among the germplasm as well as the link with the breeding program that provided the material. Diversity estimators consistently structured the population according to a priori group estimation. The phenotypic traits only discriminated main groups with contrasting characteristics, as different subspecies, ploidy level or membership in a breeding program, but were not able to discriminate within groups. A joint molecular and phenotypic characterization analysis discriminated groups based on phenotypic classification, taxonomic category, provenance source of genotypes and genetic background. Conclusions: This paper shows the significant level of diversity existing in a parental population of potato as well as the putative phylogenetic relationships among the genotypes. The use of easily measurable phenotypic traits among highly contrasting genotypes could be a reasonable approach to estimate population structure in the initial phases of a potato breeding program.
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spelling INTA48492019-04-09T11:33:40Z Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars Tagliotti, Martín Deperi, Sofia Bedogni, Maria Cecilia Zhang, Roufang Manrique Carpintero, Norma C. Coombs, Joseph Douches, David Huarte, Marcelo Atilio Papa Solanun tuberosum Fenotipos Diversidad Genética Estructura de la Población Potatoes Phenotypes Genetic Divergence Population Structure Background: Diversity in crops is fundamental for plant breeding efforts. An accurate assessment of genetic diversity, using molecular markers, such as single nucleotide polymorphism (SNP), must be able to reveal the structure of the population under study. A characterization of population structure using easy measurable phenotypic traits could be a preliminary and low-cost approach to elucidate the genetic structure of a population. A potato population of 183 genotypes was evaluated using 4859 high-quality SNPs and 19 phenotypic traits commonly recorded in potato breeding programs. A Bayesian approach, Minimum Spanning Tree (MST) and diversity estimator, as well as multivariate analysis based on phenotypic traits, were adopted to assess the population structure. Results: Analysis based on molecular markers showed groups linked to the phylogenetic relationship among the germplasm as well as the link with the breeding program that provided the material. Diversity estimators consistently structured the population according to a priori group estimation. The phenotypic traits only discriminated main groups with contrasting characteristics, as different subspecies, ploidy level or membership in a breeding program, but were not able to discriminate within groups. A joint molecular and phenotypic characterization analysis discriminated groups based on phenotypic classification, taxonomic category, provenance source of genotypes and genetic background. Conclusions: This paper shows the significant level of diversity existing in a parental population of potato as well as the putative phylogenetic relationships among the genotypes. The use of easily measurable phenotypic traits among highly contrasting genotypes could be a reasonable approach to estimate population structure in the initial phases of a potato breeding program. EEA Balcarce Fil: Tagliotti, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Deperi, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Bedogni, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Zhang, R. Inner Mongolia University. Potato Engineering and Technology Research Centre; China Fil: Manrique Carpintero, Norma C. Michigan State University. Department of Plant, Molecular Plant Sciences Bldg; Estados Unidos Fil: Coombs, Joseph. Michigan State University. Department of Plant, Molecular Plant Sciences Bldg; Estados Unidos Fil: Douches, David. Michigan State University. Department of Plant, Molecular Plant Sciences Bldg; Estados Unidos Fil: Huarte, Marcelo Atilio. INTA, Estación Experimental Agropecuaria Balcarce; Argentina. 2019-04-09T11:25:04Z 2019-04-09T11:25:04Z 2018-01-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-017-0556-9 http://hdl.handle.net/20.500.12123/4849 1471-2156 https://doi.org/10.1186/s12863-017-0556-9 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature BMC Genetics 19 (1) : 8 (January 2018)
spellingShingle Papa
Solanun tuberosum
Fenotipos
Diversidad Genética
Estructura de la Población
Potatoes
Phenotypes
Genetic Divergence
Population Structure
Tagliotti, Martín
Deperi, Sofia
Bedogni, Maria Cecilia
Zhang, Roufang
Manrique Carpintero, Norma C.
Coombs, Joseph
Douches, David
Huarte, Marcelo Atilio
Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
title Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
title_full Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
title_fullStr Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
title_full_unstemmed Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
title_short Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
title_sort use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars
topic Papa
Solanun tuberosum
Fenotipos
Diversidad Genética
Estructura de la Población
Potatoes
Phenotypes
Genetic Divergence
Population Structure
url https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-017-0556-9
http://hdl.handle.net/20.500.12123/4849
https://doi.org/10.1186/s12863-017-0556-9
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