A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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American Association of Veterinary Laboratory Diagnosticians
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/4834 https://doi.org/10.1177/1040638715582048 |
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| author | Verdes, José Manuel Márquez, Mercedes Calliari, Aldo Battes, Daniel Moraña, José Antonio Gimeno, Eduardo Juan Odriozola, Ernesto Raul Giannitti, Federico Guerrero, Florentina Fidalgo, Luis Eusebio Pumarola, Martí |
| author_browse | Battes, Daniel Calliari, Aldo Fidalgo, Luis Eusebio Giannitti, Federico Gimeno, Eduardo Juan Guerrero, Florentina Moraña, José Antonio Márquez, Mercedes Odriozola, Ernesto Raul Pumarola, Martí Verdes, José Manuel |
| author_facet | Verdes, José Manuel Márquez, Mercedes Calliari, Aldo Battes, Daniel Moraña, José Antonio Gimeno, Eduardo Juan Odriozola, Ernesto Raul Giannitti, Federico Guerrero, Florentina Fidalgo, Luis Eusebio Pumarola, Martí |
| author_sort | Verdes, José Manuel |
| collection | INTA Digital |
| description | Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4834 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Association of Veterinary Laboratory Diagnosticians |
| publisherStr | American Association of Veterinary Laboratory Diagnosticians |
| record_format | dspace |
| spelling | INTA48342022-10-31T13:12:42Z A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle Verdes, José Manuel Márquez, Mercedes Calliari, Aldo Battes, Daniel Moraña, José Antonio Gimeno, Eduardo Juan Odriozola, Ernesto Raul Giannitti, Federico Guerrero, Florentina Fidalgo, Luis Eusebio Pumarola, Martí Ganado Bovino Enfermedades de los Animales Envenenamiento Solanum Cattle Animal Diseases Poisoning Intoxicación Solanum bonariense Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death. EEA Balcarce Fil: Verdes, José Manuel. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay Fil: Márquez, Mercedes. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España Fil: Calliari, Aldo. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay Fil: Battes, Daniel. Universidad de la República. Facultad de Veterinaria; Uruguay. Fil: Moraña, José Antonio. Universidad de la República. Facultad de Veterinaria; Uruguay. Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias; Argentina Fil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Giannitti, Federico. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia; Uruguay Fil: Guerrero, Florentina. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departments of Anatomy and Animal Production; España Fil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela. Facultad de Veterinaria. Veterinary Clinical Sciences; España Fil: Pumarola, Martí. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España 2019-04-08T11:48:36Z 2019-04-08T11:48:36Z 2015-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4834 1040-6387 1943-4936 https://doi.org/10.1177/1040638715582048 eng info:eu-repo/semantics/restrictedAccess application/pdf American Association of Veterinary Laboratory Diagnosticians Journal of Veterinary Diagnostic Investigation 27 (3) : 278-286 (May 2015) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Envenenamiento Solanum Cattle Animal Diseases Poisoning Intoxicación Solanum bonariense Verdes, José Manuel Márquez, Mercedes Calliari, Aldo Battes, Daniel Moraña, José Antonio Gimeno, Eduardo Juan Odriozola, Ernesto Raul Giannitti, Federico Guerrero, Florentina Fidalgo, Luis Eusebio Pumarola, Martí A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle |
| title | A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle |
| title_full | A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle |
| title_fullStr | A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle |
| title_full_unstemmed | A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle |
| title_short | A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle |
| title_sort | novel pathogenic mechanism for cerebellar lesions produced by solanum bonariense in cattle |
| topic | Ganado Bovino Enfermedades de los Animales Envenenamiento Solanum Cattle Animal Diseases Poisoning Intoxicación Solanum bonariense |
| url | http://hdl.handle.net/20.500.12123/4834 https://doi.org/10.1177/1040638715582048 |
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