The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae

Rotavirus group A (RVA) is a major cause of diarrhea in humans and young animals including small ruminants. The purpose of this study was to identify RVA in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other RVA strains. Four out of twenty feca...

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Autores principales: Louge Uriarte, Enrique Leopoldo, Badaracco, Alejandra, Matthijnssens, Jelle, Zeller, Mark, Heylen, Elizabeth, Manazza, Jorge Alberto, Miño, Samuel, Ranst, Marc Van, Odeon, Anselmo Carlos, Parreño, Gladys Viviana
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0378113514001631
http://hdl.handle.net/20.500.12123/4824
https://doi.org/10.1016/j.vetmic.2014.03.013
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author Louge Uriarte, Enrique Leopoldo
Badaracco, Alejandra
Matthijnssens, Jelle
Zeller, Mark
Heylen, Elizabeth
Manazza, Jorge Alberto
Miño, Samuel
Ranst, Marc Van
Odeon, Anselmo Carlos
Parreño, Gladys Viviana
author_browse Badaracco, Alejandra
Heylen, Elizabeth
Louge Uriarte, Enrique Leopoldo
Manazza, Jorge Alberto
Matthijnssens, Jelle
Miño, Samuel
Odeon, Anselmo Carlos
Parreño, Gladys Viviana
Ranst, Marc Van
Zeller, Mark
author_facet Louge Uriarte, Enrique Leopoldo
Badaracco, Alejandra
Matthijnssens, Jelle
Zeller, Mark
Heylen, Elizabeth
Manazza, Jorge Alberto
Miño, Samuel
Ranst, Marc Van
Odeon, Anselmo Carlos
Parreño, Gladys Viviana
author_sort Louge Uriarte, Enrique Leopoldo
collection INTA Digital
description Rotavirus group A (RVA) is a major cause of diarrhea in humans and young animals including small ruminants. The purpose of this study was to identify RVA in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other RVA strains. Four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for RVA by ELISA. A representative sample was selected for further genome analyses. The RVA strain RVA/Goat-wt/ARG/0040/2011/G8P[1] displayed the following genomic constellation: G8-P[1]-I2-R5-C2-M2-A3-N2-T6-E12-H3, reminiscent to guanaco and other bovine-like RVA strains detected in Argentina. Phylogenetic analyses revealed that most of the genome segments had a rather close relatedness with RVA strains typically obtained from cattle, sheep, South American camelids and goats. Interestingly, strain 0040 possessed the R5 and E12 genotypes which have up to date only been found in different animal species from Argentina. Overall, these findings suggest that strain 0040 could represent a typical goat RVA genome constellation similar to those previously found in other animal species within the order Artiodactyla.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA48242019-04-05T12:41:53Z The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae Louge Uriarte, Enrique Leopoldo Badaracco, Alejandra Matthijnssens, Jelle Zeller, Mark Heylen, Elizabeth Manazza, Jorge Alberto Miño, Samuel Ranst, Marc Van Odeon, Anselmo Carlos Parreño, Gladys Viviana Caprinos Enfermedades de los Animales Rotavirus Genómica Identificación Goats Animal Diseases Genomics Identification Argentina Rotavirus group A (RVA) is a major cause of diarrhea in humans and young animals including small ruminants. The purpose of this study was to identify RVA in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other RVA strains. Four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for RVA by ELISA. A representative sample was selected for further genome analyses. The RVA strain RVA/Goat-wt/ARG/0040/2011/G8P[1] displayed the following genomic constellation: G8-P[1]-I2-R5-C2-M2-A3-N2-T6-E12-H3, reminiscent to guanaco and other bovine-like RVA strains detected in Argentina. Phylogenetic analyses revealed that most of the genome segments had a rather close relatedness with RVA strains typically obtained from cattle, sheep, South American camelids and goats. Interestingly, strain 0040 possessed the R5 and E12 genotypes which have up to date only been found in different animal species from Argentina. Overall, these findings suggest that strain 0040 could represent a typical goat RVA genome constellation similar to those previously found in other animal species within the order Artiodactyla. EEA Balcarce Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Area de Producción Animal; Argentina Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Matthijnssens, Jelle. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica. Fil: Zeller, Mark. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica Fil: Heylen, Elisabeth. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica Fil: Manazza, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Ranst, Marc Van. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2019-04-05T12:21:19Z 2019-04-05T12:21:19Z 2014-05-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0378113514001631 http://hdl.handle.net/20.500.12123/4824 0378-1135 https://doi.org/10.1016/j.vetmic.2014.03.013 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Microbiology 171 (1–2) : 189-197 (June 2014)
spellingShingle Caprinos
Enfermedades de los Animales
Rotavirus
Genómica
Identificación
Goats
Animal Diseases
Genomics
Identification
Argentina
Louge Uriarte, Enrique Leopoldo
Badaracco, Alejandra
Matthijnssens, Jelle
Zeller, Mark
Heylen, Elizabeth
Manazza, Jorge Alberto
Miño, Samuel
Ranst, Marc Van
Odeon, Anselmo Carlos
Parreño, Gladys Viviana
The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae
title The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae
title_full The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae
title_fullStr The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae
title_full_unstemmed The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae
title_short The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae
title_sort first caprine rotavirus detected in argentina displays genomic features resembling virus strains infecting members of the bovidae and camelidae
topic Caprinos
Enfermedades de los Animales
Rotavirus
Genómica
Identificación
Goats
Animal Diseases
Genomics
Identification
Argentina
url https://www.sciencedirect.com/science/article/pii/S0378113514001631
http://hdl.handle.net/20.500.12123/4824
https://doi.org/10.1016/j.vetmic.2014.03.013
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