Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobac...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://academic.oup.com/femspd/article-abstract/76/6/fty063/5057472?redirectedFrom=fulltext http://hdl.handle.net/20.500.12123/4821 https://doi.org/10.1093/femspd/fty063 |
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| author | da Silva de Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. |
| author_browse | Dias da Silva, Wilmar Lasunskaia, Elena B. Oelemann, Walter M. R. Ribeiro, Efranci Seliprandy Rodriguez, Ana Barbara F. Romano, Maria Isabel da Rocha, David Gitirana da Silva de Souza, Giliane |
| author_facet | da Silva de Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. |
| author_sort | da Silva de Souza, Giliane |
| collection | INTA Digital |
| description | Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4821 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | INTA48212019-04-05T10:57:33Z Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle da Silva de Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. Paratuberculosis Técnicas Inmunológicas Heces Mycobacterium avium Inmunodiagnóstico Ganado bovino Immunological Techniques Faeces Immunodiagnosis Cattle Fecal Biomarker Biomarcador Fecal Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis. Instituto de Biotecnología Fil: da Silva de Souza, Giliane. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil Fil: Rodriguez, Ana Barbara F. Universidade Estadual do Norte Fluminense. Laboratory of Animal Morphology and Pathology; Brasil Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil Fil: Oelemann, Walter M. R. Federal University of Rio de Janeiro. Paulo de Goes Microbiology Institute; Brasil Fil: da Rocha, David Gitirana. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil Fil: Dias da Silva, Wilmar. Butantan Institute. Immunochemistry Laboratory; Brasil Fil: Lasunskaia, Elena B. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil 2019-04-05T10:53:25Z 2019-04-05T10:53:25Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/femspd/article-abstract/76/6/fty063/5057472?redirectedFrom=fulltext http://hdl.handle.net/20.500.12123/4821 2049-632X https://doi.org/10.1093/femspd/fty063 eng info:eu-repo/semantics/restrictedAccess application/pdf Oxford University Press Pathogens and Disease 76 (6): fty063 (Agosto 2018) |
| spellingShingle | Paratuberculosis Técnicas Inmunológicas Heces Mycobacterium avium Inmunodiagnóstico Ganado bovino Immunological Techniques Faeces Immunodiagnosis Cattle Fecal Biomarker Biomarcador Fecal da Silva de Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title | Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_full | Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_fullStr | Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_full_unstemmed | Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_short | Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_sort | identification of the apa protein secreted by mycobacterium avium subsp paratuberculosis as a novel fecal biomarker for johne s disease in cattle |
| topic | Paratuberculosis Técnicas Inmunológicas Heces Mycobacterium avium Inmunodiagnóstico Ganado bovino Immunological Techniques Faeces Immunodiagnosis Cattle Fecal Biomarker Biomarcador Fecal |
| url | https://academic.oup.com/femspd/article-abstract/76/6/fty063/5057472?redirectedFrom=fulltext http://hdl.handle.net/20.500.12123/4821 https://doi.org/10.1093/femspd/fty063 |
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