Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promisin...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
PLOS
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4809 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800 https://doi.org/10.1371/journal.pone.0072800 |
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| author | Mignaqui, Ana Clara Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres |
| author_browse | Chahal, Parminder Singh Durocher, Yves Gnazzo, Victoria Mignaqui, Ana Clara Perret, Sylvie Ruiz, Vanesa Sammarruco, Romina Ayelén St-Laurent, Gilles Transfiguracion, Julia Wigdorovitz, Andres |
| author_facet | Mignaqui, Ana Clara Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres |
| author_sort | Mignaqui, Ana Clara |
| collection | INTA Digital |
| description | Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. |
| format | Artículo |
| id | INTA4809 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA48092019-04-03T17:43:24Z Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids Mignaqui, Ana Clara Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres Expresión Génica Mamíferos Virus Fiebre Aftosa Recombinación Transfección Vacuna Proteasas Gene Expression Mammals Aphthovirus Recombination Transfection Vaccines Proteases Foot and Mouth Disease Virus Capsids Cápsidas Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. Instituto de Virología Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-04-03T17:36:27Z 2019-04-03T17:36:27Z 2013-08-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4809 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800 1932-6203 https://doi.org/10.1371/journal.pone.0072800 eng info:eu-repograntAgreement/INTA/AESA/201721/AR./Fiebre aftosa info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 8 (8) : e72800. (August 20, 2013) |
| spellingShingle | Expresión Génica Mamíferos Virus Fiebre Aftosa Recombinación Transfección Vacuna Proteasas Gene Expression Mammals Aphthovirus Recombination Transfection Vaccines Proteases Foot and Mouth Disease Virus Capsids Cápsidas Mignaqui, Ana Clara Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
| title | Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
| title_full | Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
| title_fullStr | Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
| title_full_unstemmed | Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
| title_short | Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
| title_sort | transient gene expression in serum free suspension growing mammalian cells for the production of foot and mouth disease virus empty capsids |
| topic | Expresión Génica Mamíferos Virus Fiebre Aftosa Recombinación Transfección Vacuna Proteasas Gene Expression Mammals Aphthovirus Recombination Transfection Vaccines Proteases Foot and Mouth Disease Virus Capsids Cápsidas |
| url | http://hdl.handle.net/20.500.12123/4809 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800 https://doi.org/10.1371/journal.pone.0072800 |
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