Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading t...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
BMC
2019
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4806 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y https://doi.org/10.1186/s12870-019-1709-y |
| _version_ | 1855483500452380672 |
|---|---|
| author | De Haro, Luis Alejandro Arellano, Sofía Maité Novak, Ondrej Feil, Regina Dumon, Analia Delfina Mattio, Maria Fernanda Tarkowska, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John Edward Pearce, Stephen Figueroa, Carlos María Del Vas, Mariana |
| author_browse | Arellano, Sofía Maité De Haro, Luis Alejandro Del Vas, Mariana Dumon, Analia Delfina Feil, Regina Figueroa, Carlos María Llauger, Gabriela Lunn, John Edward Mattio, Maria Fernanda Novak, Ondrej Pearce, Stephen Strnad, Miroslav Tarkowska, Danuse |
| author_facet | De Haro, Luis Alejandro Arellano, Sofía Maité Novak, Ondrej Feil, Regina Dumon, Analia Delfina Mattio, Maria Fernanda Tarkowska, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John Edward Pearce, Stephen Figueroa, Carlos María Del Vas, Mariana |
| author_sort | De Haro, Luis Alejandro |
| collection | INTA Digital |
| description | Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements.
Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves.
Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants. |
| format | Artículo |
| id | INTA4806 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA48062024-08-01T10:30:36Z Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves De Haro, Luis Alejandro Arellano, Sofía Maité Novak, Ondrej Feil, Regina Dumon, Analia Delfina Mattio, Maria Fernanda Tarkowska, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John Edward Pearce, Stephen Figueroa, Carlos María Del Vas, Mariana Symptoms Fijivirus Sustancias de Crecimiento Vegetal Reoviridae Trehalosa Trigo Síntomas Plant Growth Substances Trehalose Wheat Mal de Río Cuarto virus Plant Hormones Sucrose Metabolism Virus del mal de Río Cuarto Hormonas Vegetales Metabolismo de la Sacarosa Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants. Instituto de Biotecnología Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Novak, Ondrej. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Dumon, Analia Delina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Tarkowska, Danuse. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Strnad, Miroslav. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa Fil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidos Fil: Figueroa, Carlos María. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-04-03T16:43:30Z 2019-04-03T16:43:30Z 2019-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4806 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y 1471-2229 https://doi.org/10.1186/s12870-019-1709-y eng info:eu-repograntAgreement/INTA/PNBIO/1131022/AR./Genómica funcional y biología de sistemas. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC BMC Plant Biology 19 : 112 (Marzo 2019) |
| spellingShingle | Symptoms Fijivirus Sustancias de Crecimiento Vegetal Reoviridae Trehalosa Trigo Síntomas Plant Growth Substances Trehalose Wheat Mal de Río Cuarto virus Plant Hormones Sucrose Metabolism Virus del mal de Río Cuarto Hormonas Vegetales Metabolismo de la Sacarosa De Haro, Luis Alejandro Arellano, Sofía Maité Novak, Ondrej Feil, Regina Dumon, Analia Delfina Mattio, Maria Fernanda Tarkowska, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John Edward Pearce, Stephen Figueroa, Carlos María Del Vas, Mariana Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title | Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_full | Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_fullStr | Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_full_unstemmed | Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_short | Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_sort | mal de rio cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| topic | Symptoms Fijivirus Sustancias de Crecimiento Vegetal Reoviridae Trehalosa Trigo Síntomas Plant Growth Substances Trehalose Wheat Mal de Río Cuarto virus Plant Hormones Sucrose Metabolism Virus del mal de Río Cuarto Hormonas Vegetales Metabolismo de la Sacarosa |
| url | http://hdl.handle.net/20.500.12123/4806 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y https://doi.org/10.1186/s12870-019-1709-y |
| work_keys_str_mv | AT deharoluisalejandro malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT arellanosofiamaite malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT novakondrej malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT feilregina malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT dumonanaliadelfina malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT mattiomariafernanda malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT tarkowskadanuse malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT llaugergabriela malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT strnadmiroslav malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT lunnjohnedward malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT pearcestephen malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT figueroacarlosmaria malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves AT delvasmariana malderiocuartovirusinfectioncauseshormoneimbalanceandsugaraccumulationinwheatleaves |