Clostridium perfringens type E virulence traits involved in gut colonization

Clostridium perfringens type E disease in ruminants has been characterized by hemorrhagic enteritis or sudden death. Although type E isolates are defined by the production of alpha and iota toxin, little is known about the pathogenesis of C. perfringens type E infections. Thus far, the role of iota...

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Main Authors: Redondo, Leandro Martín, Diaz Carrasco, Juan María, Redondo, Enzo Alejandro, Delgado, Fernando Oscar, Fernandez Miyakawa, Mariano Enrique
Format: Artículo
Language:Inglés
Published: PLOS 2019
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/4804
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121305
https://doi.org/10.1371/journal.pone.0121305
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author Redondo, Leandro Martín
Diaz Carrasco, Juan María
Redondo, Enzo Alejandro
Delgado, Fernando Oscar
Fernandez Miyakawa, Mariano Enrique
author_browse Delgado, Fernando Oscar
Diaz Carrasco, Juan María
Fernandez Miyakawa, Mariano Enrique
Redondo, Enzo Alejandro
Redondo, Leandro Martín
author_facet Redondo, Leandro Martín
Diaz Carrasco, Juan María
Redondo, Enzo Alejandro
Delgado, Fernando Oscar
Fernandez Miyakawa, Mariano Enrique
author_sort Redondo, Leandro Martín
collection INTA Digital
description Clostridium perfringens type E disease in ruminants has been characterized by hemorrhagic enteritis or sudden death. Although type E isolates are defined by the production of alpha and iota toxin, little is known about the pathogenesis of C. perfringens type E infections. Thus far, the role of iota toxin as a virulence factor is unknown. In this report, iota toxin showed positive effects on adherence and colonization of C. perfringens type E while having negative effect on the adherence of type A cells. In-vitro and in-vivo models suggest that toxinotype E would be particularly adapted to exploit the changes induced by iota toxin in the surface of epithelial cells. In addition, type E strains produce metabolites that affected the growth of potential intra-specific competitors. These results suggest that the alteration of the enterocyte morphology induced by iota toxin concomitantly with the specific increase of type E cell adhesion and the strong intra-specific growth inhibition of other strains could be competitive traits inherent to type E isolates that improve its fitness within the bovine gut environment.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2019
publishDateRange 2019
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spelling INTA48042019-04-03T12:00:55Z Clostridium perfringens type E virulence traits involved in gut colonization Redondo, Leandro Martín Diaz Carrasco, Juan María Redondo, Enzo Alejandro Delgado, Fernando Oscar Fernandez Miyakawa, Mariano Enrique Clostridium Perfringens Virulencia Enfermedades Gastrointestinales Bacteriocinas Enteritis Virulence Gastrointestinal Diseases Bacteriocins Gut Colonization Colonización Intestinal Clostridium perfringens type E disease in ruminants has been characterized by hemorrhagic enteritis or sudden death. Although type E isolates are defined by the production of alpha and iota toxin, little is known about the pathogenesis of C. perfringens type E infections. Thus far, the role of iota toxin as a virulence factor is unknown. In this report, iota toxin showed positive effects on adherence and colonization of C. perfringens type E while having negative effect on the adherence of type A cells. In-vitro and in-vivo models suggest that toxinotype E would be particularly adapted to exploit the changes induced by iota toxin in the surface of epithelial cells. In addition, type E strains produce metabolites that affected the growth of potential intra-specific competitors. These results suggest that the alteration of the enterocyte morphology induced by iota toxin concomitantly with the specific increase of type E cell adhesion and the strong intra-specific growth inhibition of other strains could be competitive traits inherent to type E isolates that improve its fitness within the bovine gut environment. Instituto de Patobiología Fil: Redondo, Leandro Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Diaz Carrasco, Juan María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Redondo, Enzo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-04-03T11:56:38Z 2019-04-03T11:56:38Z 2015-03-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4804 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121305 1932-6203 https://doi.org/10.1371/journal.pone.0121305 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 10 (3) : e0121305. (March 23, 2015)
spellingShingle Clostridium Perfringens
Virulencia
Enfermedades Gastrointestinales
Bacteriocinas
Enteritis
Virulence
Gastrointestinal Diseases
Bacteriocins
Gut Colonization
Colonización Intestinal
Redondo, Leandro Martín
Diaz Carrasco, Juan María
Redondo, Enzo Alejandro
Delgado, Fernando Oscar
Fernandez Miyakawa, Mariano Enrique
Clostridium perfringens type E virulence traits involved in gut colonization
title Clostridium perfringens type E virulence traits involved in gut colonization
title_full Clostridium perfringens type E virulence traits involved in gut colonization
title_fullStr Clostridium perfringens type E virulence traits involved in gut colonization
title_full_unstemmed Clostridium perfringens type E virulence traits involved in gut colonization
title_short Clostridium perfringens type E virulence traits involved in gut colonization
title_sort clostridium perfringens type e virulence traits involved in gut colonization
topic Clostridium Perfringens
Virulencia
Enfermedades Gastrointestinales
Bacteriocinas
Enteritis
Virulence
Gastrointestinal Diseases
Bacteriocins
Gut Colonization
Colonización Intestinal
url http://hdl.handle.net/20.500.12123/4804
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121305
https://doi.org/10.1371/journal.pone.0121305
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