Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer
There are increasing evidences that horizontal gene transfer (HGT) is a critical mechanism of bacterial evolution, while its complete impact remains unclear. A main constraint of HGT effects on microbial evolution seems to be the conservation of the function of the horizontally transferred genes. Fr...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/4796 https://link.springer.com/article/10.1007%2Fs00248-018-1163-7 https://doi.org/10.1007/s00248-018-1163-7 |
| _version_ | 1855483498526146560 |
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| author | Stritzler, Margarita Soto, Gabriela Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Soto, Gabriela Stritzler, Margarita |
| author_facet | Stritzler, Margarita Soto, Gabriela Ayub, Nicolás Daniel |
| author_sort | Stritzler, Margarita |
| collection | INTA Digital |
| description | There are increasing evidences that horizontal gene transfer (HGT) is a critical mechanism of bacterial evolution, while its complete impact remains unclear. A main constraint of HGT effects on microbial evolution seems to be the conservation of the function of the horizontally transferred genes. From this perspective, inflexible nomenclature and functionality criteria have been established for some mobile genetic elements such as pathogenic and symbiotic islands. Adhesion is a universal prerequisite for both beneficial and pathogenic plant-microbe interactions, and thus, adhesion systems (e.g., the Lap cluster) are candidates to have a dual function depending on the genomic background. In this study, we showed that the virulent factor Lap of the phytopathogen Erwinia carotovora SCRI1043, which is located within a genomic island, was acquired by HGT and probably derived from Pseudomonas. The transformation of the phytopathogen Erwinia pyrifoliae Ep1/96 with the beneficial factor Lap from the plant growth-promoting bacterium Pseudomonas fluorescens Pf-5 significantly increased its natural virulence, experimentally recapitulating the beneficial-to-virulence functional switch of the Lap cluster via HGT. To our knowledge, this is the first report of a functional switch of an individual gene or a cluster of genes mediated by HGT. |
| format | Artículo |
| id | INTA4796 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA47962019-04-01T13:27:50Z Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer Stritzler, Margarita Soto, Gabriela Ayub, Nicolás Daniel Genes Evolución Erwinia Pseudomonas Transferencia de Genes Growth Promoters Evolution Gene Transfer Lap Cluster Horizontal Transfer HGT There are increasing evidences that horizontal gene transfer (HGT) is a critical mechanism of bacterial evolution, while its complete impact remains unclear. A main constraint of HGT effects on microbial evolution seems to be the conservation of the function of the horizontally transferred genes. From this perspective, inflexible nomenclature and functionality criteria have been established for some mobile genetic elements such as pathogenic and symbiotic islands. Adhesion is a universal prerequisite for both beneficial and pathogenic plant-microbe interactions, and thus, adhesion systems (e.g., the Lap cluster) are candidates to have a dual function depending on the genomic background. In this study, we showed that the virulent factor Lap of the phytopathogen Erwinia carotovora SCRI1043, which is located within a genomic island, was acquired by HGT and probably derived from Pseudomonas. The transformation of the phytopathogen Erwinia pyrifoliae Ep1/96 with the beneficial factor Lap from the plant growth-promoting bacterium Pseudomonas fluorescens Pf-5 significantly increased its natural virulence, experimentally recapitulating the beneficial-to-virulence functional switch of the Lap cluster via HGT. To our knowledge, this is the first report of a functional switch of an individual gene or a cluster of genes mediated by HGT. Instituto de Genética Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-04-01T13:21:37Z 2019-04-01T13:21:37Z 2018-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4796 https://link.springer.com/article/10.1007%2Fs00248-018-1163-7 0095-3628 1432-184X (Online) https://doi.org/10.1007/s00248-018-1163-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Microbial ecology 76 (3) : 579–583. (October 2018) |
| spellingShingle | Genes Evolución Erwinia Pseudomonas Transferencia de Genes Growth Promoters Evolution Gene Transfer Lap Cluster Horizontal Transfer HGT Stritzler, Margarita Soto, Gabriela Ayub, Nicolás Daniel Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer |
| title | Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer |
| title_full | Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer |
| title_fullStr | Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer |
| title_full_unstemmed | Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer |
| title_short | Plant growth-promoting genes can switch to be virulence factors via horizontal gene transfer |
| title_sort | plant growth promoting genes can switch to be virulence factors via horizontal gene transfer |
| topic | Genes Evolución Erwinia Pseudomonas Transferencia de Genes Growth Promoters Evolution Gene Transfer Lap Cluster Horizontal Transfer HGT |
| url | http://hdl.handle.net/20.500.12123/4796 https://link.springer.com/article/10.1007%2Fs00248-018-1163-7 https://doi.org/10.1007/s00248-018-1163-7 |
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