Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle
The aim of this study was to evaluate delayed type hypersensitivity (DTH) induced by the intradermal inoculation of a Neospora caninum tachyzoite soluble lysate in cattle previously exposed with the protozoa. Four experimental groups were selected according to the prior exposure to N. caninum antige...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0165242718302150 http://hdl.handle.net/20.500.12123/4795 https://doi.org/10.1016/j.vetimm.2018.11.011 |
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| author | Fiorani, Franco Armendano, Joaquín Ignacio Hecker, Yanina Paola Araoz, Virgina Canton, German Jose Leunda, Maria Rosa Pereyra, Susana Beatriz Corva, Pablo Marcelo Odeon, Anselmo Carlos Moore, Prando Dadin |
| author_browse | Araoz, Virgina Armendano, Joaquín Ignacio Canton, German Jose Corva, Pablo Marcelo Fiorani, Franco Hecker, Yanina Paola Leunda, Maria Rosa Moore, Prando Dadin Odeon, Anselmo Carlos Pereyra, Susana Beatriz |
| author_facet | Fiorani, Franco Armendano, Joaquín Ignacio Hecker, Yanina Paola Araoz, Virgina Canton, German Jose Leunda, Maria Rosa Pereyra, Susana Beatriz Corva, Pablo Marcelo Odeon, Anselmo Carlos Moore, Prando Dadin |
| author_sort | Fiorani, Franco |
| collection | INTA Digital |
| description | The aim of this study was to evaluate delayed type hypersensitivity (DTH) induced by the intradermal inoculation of a Neospora caninum tachyzoite soluble lysate in cattle previously exposed with the protozoa. Four experimental groups were selected according to the prior exposure to N. caninum antigen. All cows were intradermally injected with a N. caninum tachyzoite soluble lysate and skinfold thickness growth at the inoculation sites was measured at 0, 24, 48, 72 and 96 h post inoculation (hpi). Additionally, specific antibodies and IFN-γ production were assessed. Cows experimentally infected with live N. caninum tachyzoites and cows naturally exposed to N. caninum developed skin reactions compatible with DTH between 24 and 96 hpi (p < 0.05). Moreover, cows inoculated with an experimental N. caninum vaccine and cows without evidence of exposure to N. caninum did not show a significant increase in skin thickness (p > 0.05). Furthermore, serological status of the animals was not modified due to the intradermal inoculation. The highest IFN-γ production was observed at 15 days after intradermal inoculation (p < 0.05). Therefore, these results suggest that cattle previously exposed to N. caninum develop a reaction compatible with DTH which could be useful as in vivo cell mediated immunity parameter for assessed bovine neosporosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4795 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA47952019-06-11T14:30:40Z Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle Fiorani, Franco Armendano, Joaquín Ignacio Hecker, Yanina Paola Araoz, Virgina Canton, German Jose Leunda, Maria Rosa Pereyra, Susana Beatriz Corva, Pablo Marcelo Odeon, Anselmo Carlos Moore, Prando Dadin Ganado Bovino Neospora Caninum Hipersensibilidad Retardada Inoculación Enfermedades Parasitarias Cattle Delayed Hypersensitivity Inoculation Parasitoses The aim of this study was to evaluate delayed type hypersensitivity (DTH) induced by the intradermal inoculation of a Neospora caninum tachyzoite soluble lysate in cattle previously exposed with the protozoa. Four experimental groups were selected according to the prior exposure to N. caninum antigen. All cows were intradermally injected with a N. caninum tachyzoite soluble lysate and skinfold thickness growth at the inoculation sites was measured at 0, 24, 48, 72 and 96 h post inoculation (hpi). Additionally, specific antibodies and IFN-γ production were assessed. Cows experimentally infected with live N. caninum tachyzoites and cows naturally exposed to N. caninum developed skin reactions compatible with DTH between 24 and 96 hpi (p < 0.05). Moreover, cows inoculated with an experimental N. caninum vaccine and cows without evidence of exposure to N. caninum did not show a significant increase in skin thickness (p > 0.05). Furthermore, serological status of the animals was not modified due to the intradermal inoculation. The highest IFN-γ production was observed at 15 days after intradermal inoculation (p < 0.05). Therefore, these results suggest that cattle previously exposed to N. caninum develop a reaction compatible with DTH which could be useful as in vivo cell mediated immunity parameter for assessed bovine neosporosis. EEA Balcarce Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas;, Argentina Fil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Araoz, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Canton, German Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Leunda, María Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Corva, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina 2019-04-01T13:09:53Z 2019-04-01T13:09:53Z 2019-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0165242718302150 http://hdl.handle.net/20.500.12123/4795 0165-2427 1873-2534 https://doi.org/10.1016/j.vetimm.2018.11.011 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 207 : 31-35 (January 2019) |
| spellingShingle | Ganado Bovino Neospora Caninum Hipersensibilidad Retardada Inoculación Enfermedades Parasitarias Cattle Delayed Hypersensitivity Inoculation Parasitoses Fiorani, Franco Armendano, Joaquín Ignacio Hecker, Yanina Paola Araoz, Virgina Canton, German Jose Leunda, Maria Rosa Pereyra, Susana Beatriz Corva, Pablo Marcelo Odeon, Anselmo Carlos Moore, Prando Dadin Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle |
| title | Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle |
| title_full | Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle |
| title_fullStr | Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle |
| title_full_unstemmed | Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle |
| title_short | Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle |
| title_sort | delayed type hypersensitivity induced by intradermal inoculation of a neospora caninum tachyzoite antigen in previously exposed cattle |
| topic | Ganado Bovino Neospora Caninum Hipersensibilidad Retardada Inoculación Enfermedades Parasitarias Cattle Delayed Hypersensitivity Inoculation Parasitoses |
| url | https://www.sciencedirect.com/science/article/pii/S0165242718302150 http://hdl.handle.net/20.500.12123/4795 https://doi.org/10.1016/j.vetimm.2018.11.011 |
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