Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases
The vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix nitrogen by the expression of horizontally acquired nitrogenase, little is known a...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4793 https://link.springer.com/article/10.1007/s00239-015-9698-4 |
| _version_ | 1855483497959915520 |
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| author | Pascuan, Cecilia Gabriela Fox, Ana Romina Soto, Gabriela Cinthia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Fox, Ana Romina Pascuan, Cecilia Gabriela Soto, Gabriela Cinthia |
| author_facet | Pascuan, Cecilia Gabriela Fox, Ana Romina Soto, Gabriela Cinthia Ayub, Nicolás Daniel |
| author_sort | Pascuan, Cecilia Gabriela |
| collection | INTA Digital |
| description | The vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix nitrogen by the expression of horizontally acquired nitrogenase, little is known about the mechanisms of nitrogenase adaptation to the new bacterial host. Recently, we transferred the nitrogen fixation island from Pseudomonas stutzeri A1501 to the non-nitrogen-fixing bacterium Pseudomonas protegens Pf-5, and interestingly, the resulting recombinant strain Pf-5 X940 showed an uncommon phenotype of constitutive nitrogenase activity. Here, we integrated evolutionary and functional approaches to elucidate this unusual phenotype. Phylogenetic analysis showed that polyhydroxybutyrate (PHB) biosynthesis genes from natural nitrogen-fixing Pseudomonas strains have been acquired by horizontal transfer. Contrary to Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity under nitrogen-excess conditions, and displayed the typical switch-on phenotype observed in natural nitrogen-fixing strains after nitrogen deficiency. This indicates a competition between PHB production and nitrogen fixation. Therefore, we propose that horizontal transfer of PHB biosynthesis genes could be an ancestral mechanism of regulation of horizontally acquired nitrogenases in the genus Pseudomonas. |
| format | Artículo |
| id | INTA4793 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA47932019-04-01T14:34:20Z Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases Pascuan, Cecilia Gabriela Fox, Ana Romina Soto, Gabriela Cinthia Ayub, Nicolás Daniel Nitrogenasa Evolución Pseudomonas Nitrogenase Evolution Gene Transfer Transferencia de Genes Polyhydroxybutyrate Horizontal Transfer The vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix nitrogen by the expression of horizontally acquired nitrogenase, little is known about the mechanisms of nitrogenase adaptation to the new bacterial host. Recently, we transferred the nitrogen fixation island from Pseudomonas stutzeri A1501 to the non-nitrogen-fixing bacterium Pseudomonas protegens Pf-5, and interestingly, the resulting recombinant strain Pf-5 X940 showed an uncommon phenotype of constitutive nitrogenase activity. Here, we integrated evolutionary and functional approaches to elucidate this unusual phenotype. Phylogenetic analysis showed that polyhydroxybutyrate (PHB) biosynthesis genes from natural nitrogen-fixing Pseudomonas strains have been acquired by horizontal transfer. Contrary to Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity under nitrogen-excess conditions, and displayed the typical switch-on phenotype observed in natural nitrogen-fixing strains after nitrogen deficiency. This indicates a competition between PHB production and nitrogen fixation. Therefore, we propose that horizontal transfer of PHB biosynthesis genes could be an ancestral mechanism of regulation of horizontally acquired nitrogenases in the genus Pseudomonas. Instituto de Genética Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-04-01T12:47:21Z 2019-04-01T12:47:21Z 2015-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4793 https://link.springer.com/article/10.1007/s00239-015-9698-4 0022-2844 1432-1432 (Online) 10.1007/s00239-015-9698-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Journal of molecular evolution 81 (3–4) : 84–89. (October 2015) |
| spellingShingle | Nitrogenasa Evolución Pseudomonas Nitrogenase Evolution Gene Transfer Transferencia de Genes Polyhydroxybutyrate Horizontal Transfer Pascuan, Cecilia Gabriela Fox, Ana Romina Soto, Gabriela Cinthia Ayub, Nicolás Daniel Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| title | Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| title_full | Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| title_fullStr | Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| title_full_unstemmed | Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| title_short | Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| title_sort | exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases |
| topic | Nitrogenasa Evolución Pseudomonas Nitrogenase Evolution Gene Transfer Transferencia de Genes Polyhydroxybutyrate Horizontal Transfer |
| url | http://hdl.handle.net/20.500.12123/4793 https://link.springer.com/article/10.1007/s00239-015-9698-4 |
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