Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Acceso en línea: | https://academic.oup.com/jxb/article/70/5/1669/5307521 http://hdl.handle.net/20.500.12123/4784 https://doi.org/10.1093/jxb/erz037 |
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| author | Gonzalez, Fernanda Gabriela Capella, Matías Ribichich, Karina Fabiana Curín, Facundo Giacomelli, Jorge Ignacio Ayala, Francisco Watson, Gerónimo Otegui, María Elena Lía Chan, Raquel |
| author_browse | Ayala, Francisco Capella, Matías Curín, Facundo Giacomelli, Jorge Ignacio Gonzalez, Fernanda Gabriela Lía Chan, Raquel Otegui, María Elena Ribichich, Karina Fabiana Watson, Gerónimo |
| author_facet | Gonzalez, Fernanda Gabriela Capella, Matías Ribichich, Karina Fabiana Curín, Facundo Giacomelli, Jorge Ignacio Ayala, Francisco Watson, Gerónimo Otegui, María Elena Lía Chan, Raquel |
| author_sort | Gonzalez, Fernanda Gabriela |
| collection | INTA Digital |
| description | HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts. |
| format | Artículo |
| id | INTA4784 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | INTA47842019-03-29T17:20:36Z Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type Gonzalez, Fernanda Gabriela Capella, Matías Ribichich, Karina Fabiana Curín, Facundo Giacomelli, Jorge Ignacio Ayala, Francisco Watson, Gerónimo Otegui, María Elena Lía Chan, Raquel Helianthus annuus Genes Rendimiento Semilla Uso del Agua Transgénicos Ensayo de Semillas Sunflower Yields Seeds Water Use Transgenics Seed Testing Girasol HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts. EEA Pergamino Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento Ecofisiología; Argentina.CONICET-UNNOBA.CITNOBA; Argentina Fil: Capella, Matías. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Ribichich, Karina Fabiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Curín, Facundo. CONICET-UNNOBA. CITNOBA, (Pergamino); Argentina Fil: Giacomelli, Jorge Ignacio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Ayala, Francisco. INDER/BIOCRES (Rosario); Argentina Fil: Watson, Gerónimo. INDEAR/BIOCERES (Rosario); Argentina Fil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Lía Chan, Raquel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina 2019-03-29T17:16:19Z 2019-03-29T17:16:19Z 2019-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/jxb/article/70/5/1669/5307521 http://hdl.handle.net/20.500.12123/4784 0022-0957 1460-2431 (digital) https://doi.org/10.1093/jxb/erz037 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Experimental Botany 70 (5) : 1669–1681 (February 2019) |
| spellingShingle | Helianthus annuus Genes Rendimiento Semilla Uso del Agua Transgénicos Ensayo de Semillas Sunflower Yields Seeds Water Use Transgenics Seed Testing Girasol Gonzalez, Fernanda Gabriela Capella, Matías Ribichich, Karina Fabiana Curín, Facundo Giacomelli, Jorge Ignacio Ayala, Francisco Watson, Gerónimo Otegui, María Elena Lía Chan, Raquel Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type |
| title | Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type |
| title_full | Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type |
| title_fullStr | Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type |
| title_full_unstemmed | Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type |
| title_short | Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type |
| title_sort | field grown transgenic wheat expressing the sunflower gene hahb4 significantly outyields the wild type |
| topic | Helianthus annuus Genes Rendimiento Semilla Uso del Agua Transgénicos Ensayo de Semillas Sunflower Yields Seeds Water Use Transgenics Seed Testing Girasol |
| url | https://academic.oup.com/jxb/article/70/5/1669/5307521 http://hdl.handle.net/20.500.12123/4784 https://doi.org/10.1093/jxb/erz037 |
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