The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus
After an insect herbivore has reached its host plant, contact cues from the leaf surface often determine host acceptance. We studied contact cues during oviposition behavior of a willow pest, the sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae), a specialist feeder on Salix (Salicaceae) tree...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Nature Research
2019
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| Materias: | |
| Acceso en línea: | https://www.nature.com/articles/s41598-019-41318-7 http://hdl.handle.net/20.500.12123/4771 https://doi.org/10.1038/s41598-019-41318-7 |
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| author | Fernandez, Patricia Braccini, Celina Laura Dávila, Camila Barrozo, Romina B. Coll Araoz, Maria Victoria Cerrillo, Teresa Gershenzon, Jonathan Reichelt, Michael Zavala, Jorge Alberto |
| author_browse | Barrozo, Romina B. Braccini, Celina Laura Cerrillo, Teresa Coll Araoz, Maria Victoria Dávila, Camila Fernandez, Patricia Gershenzon, Jonathan Reichelt, Michael Zavala, Jorge Alberto |
| author_facet | Fernandez, Patricia Braccini, Celina Laura Dávila, Camila Barrozo, Romina B. Coll Araoz, Maria Victoria Cerrillo, Teresa Gershenzon, Jonathan Reichelt, Michael Zavala, Jorge Alberto |
| author_sort | Fernandez, Patricia |
| collection | INTA Digital |
| description | After an insect herbivore has reached its host plant, contact cues from the leaf surface often determine host acceptance. We studied contact cues during oviposition behavior of a willow pest, the sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae), a specialist feeder on Salix (Salicaceae) trees, and how it determines oviposition preference in lab and field conditions. We described the sequence of behaviors that lead to egg laying on the most and least preferred willow species. Then we studied the morphology of chemosensory structures present on the female antenna, cerci and ovipositor. Since phenolic glycosides (PGs) are the main secondary metabolites present in Salicaceae species, we investigated their role in host acceptance. We quantified these compounds in different willow species and correlated PG content with oviposition preference under lab and natural field conditions. We demonstrated a major role for contact cues in triggering N. oligospilus egg laying on the leaf surface of preferred willow genotypes. Firstly cues are sensed by antennae, determining to leave or stay on the leaf. After that, sensing is performed by abdominal cerci, which finally triggers egg laying. The lack of PGs in non-preferred species and the significant correlation observed between PGs, natural damage and oviposition preference suggest a role for these compounds in host selection. Our study suggests that in specialist feeders, secondary compounds normally acting as defenses can actually act as a susceptibility factor by triggering specific insect behavior for oviposition. These defensive compounds could be selected against to increase resistance. |
| format | Artículo |
| id | INTA4771 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Nature Research |
| publisherStr | Nature Research |
| record_format | dspace |
| spelling | INTA47712019-03-28T14:49:17Z The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus Fernandez, Patricia Braccini, Celina Laura Dávila, Camila Barrozo, Romina B. Coll Araoz, Maria Victoria Cerrillo, Teresa Gershenzon, Jonathan Reichelt, Michael Zavala, Jorge Alberto Insecta Nematus Plagas de Plantas Salix Comportamiento Oviposición Pests of Plants Behaviour Oviposition Nematus oligospilus Sauce (salix) After an insect herbivore has reached its host plant, contact cues from the leaf surface often determine host acceptance. We studied contact cues during oviposition behavior of a willow pest, the sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae), a specialist feeder on Salix (Salicaceae) trees, and how it determines oviposition preference in lab and field conditions. We described the sequence of behaviors that lead to egg laying on the most and least preferred willow species. Then we studied the morphology of chemosensory structures present on the female antenna, cerci and ovipositor. Since phenolic glycosides (PGs) are the main secondary metabolites present in Salicaceae species, we investigated their role in host acceptance. We quantified these compounds in different willow species and correlated PG content with oviposition preference under lab and natural field conditions. We demonstrated a major role for contact cues in triggering N. oligospilus egg laying on the leaf surface of preferred willow genotypes. Firstly cues are sensed by antennae, determining to leave or stay on the leaf. After that, sensing is performed by abdominal cerci, which finally triggers egg laying. The lack of PGs in non-preferred species and the significant correlation observed between PGs, natural damage and oviposition preference suggest a role for these compounds in host selection. Our study suggests that in specialist feeders, secondary compounds normally acting as defenses can actually act as a susceptibility factor by triggering specific insect behavior for oviposition. These defensive compounds could be selected against to increase resistance. EEA Delta del Paraná Fil: Fernandez, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Delta del Paraná; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica; Argentina Fil: Braccini, Celina Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Dávila, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica; Argentina Fil: Barrozo, Romina B. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Coll Araoz, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Cerrillo, Teresa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Delta del Paraná; Argentina Fil: Gershenzon, Jonathan. Max Planck Institute for Chemical Ecology; Alemania Fil: Reichelt, Michael. Max Planck Institute for Chemical Ecology; Alemania Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-28T14:47:54Z 2019-03-28T14:47:54Z 2019-03-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.nature.com/articles/s41598-019-41318-7 http://hdl.handle.net/20.500.12123/4771 2045-2322 https://doi.org/10.1038/s41598-019-41318-7 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Nature Research Scientific Reports 9 : article number: 4946 (2019) |
| spellingShingle | Insecta Nematus Plagas de Plantas Salix Comportamiento Oviposición Pests of Plants Behaviour Oviposition Nematus oligospilus Sauce (salix) Fernandez, Patricia Braccini, Celina Laura Dávila, Camila Barrozo, Romina B. Coll Araoz, Maria Victoria Cerrillo, Teresa Gershenzon, Jonathan Reichelt, Michael Zavala, Jorge Alberto The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus |
| title | The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus |
| title_full | The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus |
| title_fullStr | The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus |
| title_full_unstemmed | The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus |
| title_short | The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus Oligospilus |
| title_sort | use of leaf surface contact cues during oviposition explains field preferences in the willow sawfly nematus oligospilus |
| topic | Insecta Nematus Plagas de Plantas Salix Comportamiento Oviposición Pests of Plants Behaviour Oviposition Nematus oligospilus Sauce (salix) |
| url | https://www.nature.com/articles/s41598-019-41318-7 http://hdl.handle.net/20.500.12123/4771 https://doi.org/10.1038/s41598-019-41318-7 |
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