Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12048 http://hdl.handle.net/20.500.12123/4768 https://doi.org/10.1111/ppl.12048 |
| _version_ | 1855035380892434432 |
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| author | Roqueiro, Gonzalo Causin, Humberto Fabio Ollé Resa, Carolina Patricia Maroder, Horacio Maldonado, Sara Beatriz |
| author_browse | Causin, Humberto Fabio Maldonado, Sara Beatriz Maroder, Horacio Ollé Resa, Carolina Patricia Roqueiro, Gonzalo |
| author_facet | Roqueiro, Gonzalo Causin, Humberto Fabio Ollé Resa, Carolina Patricia Maroder, Horacio Maldonado, Sara Beatriz |
| author_sort | Roqueiro, Gonzalo |
| collection | INTA Digital |
| description | In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4768 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA47682019-06-06T18:04:01Z Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination Roqueiro, Gonzalo Causin, Humberto Fabio Ollé Resa, Carolina Patricia Maroder, Horacio Maldonado, Sara Beatriz Salix Plántulas Germinación Cotiledones Morfología Vegetal Seedlings Germination Cotyledons Plant Morphology Sauce (salix) Willow In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. EEA San Juan Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Causin, Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Ollé Resa, Carolina Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Maroder, Horacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina 2019-03-28T12:57:31Z 2019-03-28T12:57:31Z 2013-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12048 http://hdl.handle.net/20.500.12123/4768 0031-9317 1399-3054 https://doi.org/10.1111/ppl.12048 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Physiologia Plantarum 149 (2) : 286-295. (October 2013) |
| spellingShingle | Salix Plántulas Germinación Cotiledones Morfología Vegetal Seedlings Germination Cotyledons Plant Morphology Sauce (salix) Willow Roqueiro, Gonzalo Causin, Humberto Fabio Ollé Resa, Carolina Patricia Maroder, Horacio Maldonado, Sara Beatriz Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| title | Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| title_full | Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| title_fullStr | Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| title_full_unstemmed | Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| title_short | Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| title_sort | willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence a histological and biochemical study following germination |
| topic | Salix Plántulas Germinación Cotiledones Morfología Vegetal Seedlings Germination Cotyledons Plant Morphology Sauce (salix) Willow |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12048 http://hdl.handle.net/20.500.12123/4768 https://doi.org/10.1111/ppl.12048 |
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