Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infe...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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PLOS
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4764 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127710 https://doi.org/10.1371/journal.pone.0127710 |
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| author | Amigo, Natalia Mercado, Elsa Cristina Bentancor, Adriana Beatriz Singh, Pallavi Vilte, Daniel Alejandro Gerhardt, Elizabeth Zotta, Elsa Ibarra, Cristina Adriana Manning, Shannon D. Larzabal, Mariano Cataldi, Angel Adrian |
| author_browse | Amigo, Natalia Bentancor, Adriana Beatriz Cataldi, Angel Adrian Gerhardt, Elizabeth Ibarra, Cristina Adriana Larzabal, Mariano Manning, Shannon D. Mercado, Elsa Cristina Singh, Pallavi Vilte, Daniel Alejandro Zotta, Elsa |
| author_facet | Amigo, Natalia Mercado, Elsa Cristina Bentancor, Adriana Beatriz Singh, Pallavi Vilte, Daniel Alejandro Gerhardt, Elizabeth Zotta, Elsa Ibarra, Cristina Adriana Manning, Shannon D. Larzabal, Mariano Cataldi, Angel Adrian |
| author_sort | Amigo, Natalia |
| collection | INTA Digital |
| description | The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4764 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA47642019-03-27T17:49:09Z Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes Amigo, Natalia Mercado, Elsa Cristina Bentancor, Adriana Beatriz Singh, Pallavi Vilte, Daniel Alejandro Gerhardt, Elizabeth Zotta, Elsa Ibarra, Cristina Adriana Manning, Shannon D. Larzabal, Mariano Cataldi, Angel Adrian Escherichia Coli Ganado Bovino Fenotipos Genética Molecular Colon (Intestino) Cattle Phenotypes Molecular Genetics Colon Hemolytic-uremic Syndrome Síndrome Urémico Hemolítico Argentina The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina. Instituto de Biotecnología Fil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Microbiología; Argentina Fil: Singh, Pallavi. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Gerhardt, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina Fil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina Fil: Manning, Shannon D. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-03-27T17:45:08Z 2019-03-27T17:45:08Z 2015-06-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4764 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127710 1932-6203 https://doi.org/10.1371/journal.pone.0127710 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 10 (6) : e0127710. (June 1, 2015) |
| spellingShingle | Escherichia Coli Ganado Bovino Fenotipos Genética Molecular Colon (Intestino) Cattle Phenotypes Molecular Genetics Colon Hemolytic-uremic Syndrome Síndrome Urémico Hemolítico Argentina Amigo, Natalia Mercado, Elsa Cristina Bentancor, Adriana Beatriz Singh, Pallavi Vilte, Daniel Alejandro Gerhardt, Elizabeth Zotta, Elsa Ibarra, Cristina Adriana Manning, Shannon D. Larzabal, Mariano Cataldi, Angel Adrian Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes |
| title | Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes |
| title_full | Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes |
| title_fullStr | Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes |
| title_full_unstemmed | Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes |
| title_short | Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes |
| title_sort | clade 8 and clade 6 strains of escherichia coli o157 h7 from cattle in argentina have hypervirulent like phenotypes |
| topic | Escherichia Coli Ganado Bovino Fenotipos Genética Molecular Colon (Intestino) Cattle Phenotypes Molecular Genetics Colon Hemolytic-uremic Syndrome Síndrome Urémico Hemolítico Argentina |
| url | http://hdl.handle.net/20.500.12123/4764 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127710 https://doi.org/10.1371/journal.pone.0127710 |
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