Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1...
| Main Authors: | , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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PLOS
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4762 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214165 https://doi.org/10.1371/journal.pone.0214165 |
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| author | Nahirñak, Vanesa Rivarola, Maximo Lisandro Almasia, Natalia Ines Barrios Baron, María Pilar Hopp, Horacio Esteban Vile, Denis Paniego, Norma Beatriz Vazquez Rovere, Cecilia |
| author_browse | Almasia, Natalia Ines Barrios Baron, María Pilar Hopp, Horacio Esteban Nahirñak, Vanesa Paniego, Norma Beatriz Rivarola, Maximo Lisandro Vazquez Rovere, Cecilia Vile, Denis |
| author_facet | Nahirñak, Vanesa Rivarola, Maximo Lisandro Almasia, Natalia Ines Barrios Baron, María Pilar Hopp, Horacio Esteban Vile, Denis Paniego, Norma Beatriz Vazquez Rovere, Cecilia |
| author_sort | Nahirñak, Vanesa |
| collection | INTA Digital |
| description | Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4762 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA47622019-06-05T17:05:56Z Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato Nahirñak, Vanesa Rivarola, Maximo Lisandro Almasia, Natalia Ines Barrios Baron, María Pilar Hopp, Horacio Esteban Vile, Denis Paniego, Norma Beatriz Vazquez Rovere, Cecilia Papa Solanum Tuberosum Acido Ascórbico Hormonas Esteroles Metabolismo Oxirredución Especies de Oxígeno Reactivo Potatoes Ascorbic Acid Hormones Sterols Metabolism Oxidoreductions Reactive Oxygen Species Snakin-1 Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones. Instituto de Biotecnología Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Barrios Barón, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vile, Denis. Institute National de la Recherche Agronomique. Laboratoire d'écophysiologie des Plantes sous Stress Environnementaux; Francia; Université de Montpellier; Francia Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA).Labintex Europa; Francia 2019-03-27T16:39:50Z 2019-03-27T16:39:50Z 2019-03-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4762 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214165 1932-6203 https://doi.org/10.1371/journal.pone.0214165 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 14 (3) : e0214165. (March 25, 2019) |
| spellingShingle | Papa Solanum Tuberosum Acido Ascórbico Hormonas Esteroles Metabolismo Oxirredución Especies de Oxígeno Reactivo Potatoes Ascorbic Acid Hormones Sterols Metabolism Oxidoreductions Reactive Oxygen Species Snakin-1 Nahirñak, Vanesa Rivarola, Maximo Lisandro Almasia, Natalia Ines Barrios Baron, María Pilar Hopp, Horacio Esteban Vile, Denis Paniego, Norma Beatriz Vazquez Rovere, Cecilia Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| title | Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| title_full | Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| title_fullStr | Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| title_full_unstemmed | Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| title_short | Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| title_sort | snakin 1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato |
| topic | Papa Solanum Tuberosum Acido Ascórbico Hormonas Esteroles Metabolismo Oxirredución Especies de Oxígeno Reactivo Potatoes Ascorbic Acid Hormones Sterols Metabolism Oxidoreductions Reactive Oxygen Species Snakin-1 |
| url | http://hdl.handle.net/20.500.12123/4762 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214165 https://doi.org/10.1371/journal.pone.0214165 |
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