Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes fi...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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PLOS
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4759 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107117 https://doi.org/10.1371/journal.pone.0107117 |
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| author | Golan, Ido Dominguez, Pia Guadalupe Konrad, Zvia Shkolnik Inbar, Doron Carrari, Fernando Bar Zvi, Dudy |
| author_browse | Bar Zvi, Dudy Carrari, Fernando Dominguez, Pia Guadalupe Golan, Ido Konrad, Zvia Shkolnik Inbar, Doron |
| author_facet | Golan, Ido Dominguez, Pia Guadalupe Konrad, Zvia Shkolnik Inbar, Doron Carrari, Fernando Bar Zvi, Dudy |
| author_sort | Golan, Ido |
| collection | INTA Digital |
| description | Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes five ASR genes: ASR1, 2, 3 and 5 encode low-molecular-weight proteins (ca. 110 amino acid residues each), whereas ASR4 encodes a 297-residue polypeptide. Information on the expression of the tomato ASR gene family is scarce. We used quantitative RT-PCR to assay the expression of this gene family in plant development and in response to salt and osmotic stresses. ASR1 and ASR4 were the main expressed genes in all tested organs and conditions, whereas ASR2 and ASR3/5 expression was two to three orders of magnitude lower (with the exception of cotyledons). ASR1 is expressed in all plant tissues tested whereas ASR4 expression is limited to photosynthetic organs and stamens. Essentially, ASR1 accounted for most of ASR gene expression in roots, stems and fruits at all developmental stages, whereas ASR4 was the major gene expressed in cotyledons and young and fully developed leaves. Both ASR1 and ASR4 were expressed in flower organs, with ASR1 expression dominating in stamens and pistils, ASR4 in sepals and petals. Steady-state levels of ASR1 and ASR4 were upregulated in plant vegetative organs following exposure to salt stress, osmotic stress or the plant abiotic stress hormone abscisic acid (ABA). Tomato plants overexpressing ASR1 displayed enhanced survival rates under conditions of water stress, whereas ASR1-antisense plants displayed marginal hypersensitivity to water withholding. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4759 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA47592019-03-27T14:52:14Z Tomato Abscisic Acid Stress ripening (ASR) gene family revisited Golan, Ido Dominguez, Pia Guadalupe Konrad, Zvia Shkolnik Inbar, Doron Carrari, Fernando Bar Zvi, Dudy Tomate Solanum Lycopersicum Hojas Fruto Expresión Génica Maduración Estrés Abiótico ABA Estrés Osmótico Plantas Transgénicas Resistencia Fisiológica al Estrés Tomatoes Leaves Fruit Gene Expression Maturation Abiotic Stress Osmotic Stress Transgenic Plants Physiological Stress Resistance Ácido Abscísico Abscisic Acid Asr1 Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes five ASR genes: ASR1, 2, 3 and 5 encode low-molecular-weight proteins (ca. 110 amino acid residues each), whereas ASR4 encodes a 297-residue polypeptide. Information on the expression of the tomato ASR gene family is scarce. We used quantitative RT-PCR to assay the expression of this gene family in plant development and in response to salt and osmotic stresses. ASR1 and ASR4 were the main expressed genes in all tested organs and conditions, whereas ASR2 and ASR3/5 expression was two to three orders of magnitude lower (with the exception of cotyledons). ASR1 is expressed in all plant tissues tested whereas ASR4 expression is limited to photosynthetic organs and stamens. Essentially, ASR1 accounted for most of ASR gene expression in roots, stems and fruits at all developmental stages, whereas ASR4 was the major gene expressed in cotyledons and young and fully developed leaves. Both ASR1 and ASR4 were expressed in flower organs, with ASR1 expression dominating in stamens and pistils, ASR4 in sepals and petals. Steady-state levels of ASR1 and ASR4 were upregulated in plant vegetative organs following exposure to salt stress, osmotic stress or the plant abiotic stress hormone abscisic acid (ABA). Tomato plants overexpressing ASR1 displayed enhanced survival rates under conditions of water stress, whereas ASR1-antisense plants displayed marginal hypersensitivity to water withholding. Instituto de Biotecnología Fil: Golan, Ido. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Konrad, Zvia. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel Fil: Shkolnik Inbar, Doron. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel 2019-03-27T14:45:25Z 2019-03-27T14:45:25Z 2014-10-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4759 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107117 1932-6203 https://doi.org/10.1371/journal.pone.0107117 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 9 (10) : e107117. (October 13, 2014) |
| spellingShingle | Tomate Solanum Lycopersicum Hojas Fruto Expresión Génica Maduración Estrés Abiótico ABA Estrés Osmótico Plantas Transgénicas Resistencia Fisiológica al Estrés Tomatoes Leaves Fruit Gene Expression Maturation Abiotic Stress Osmotic Stress Transgenic Plants Physiological Stress Resistance Ácido Abscísico Abscisic Acid Asr1 Golan, Ido Dominguez, Pia Guadalupe Konrad, Zvia Shkolnik Inbar, Doron Carrari, Fernando Bar Zvi, Dudy Tomato Abscisic Acid Stress ripening (ASR) gene family revisited |
| title | Tomato Abscisic Acid Stress ripening (ASR) gene family revisited |
| title_full | Tomato Abscisic Acid Stress ripening (ASR) gene family revisited |
| title_fullStr | Tomato Abscisic Acid Stress ripening (ASR) gene family revisited |
| title_full_unstemmed | Tomato Abscisic Acid Stress ripening (ASR) gene family revisited |
| title_short | Tomato Abscisic Acid Stress ripening (ASR) gene family revisited |
| title_sort | tomato abscisic acid stress ripening asr gene family revisited |
| topic | Tomate Solanum Lycopersicum Hojas Fruto Expresión Génica Maduración Estrés Abiótico ABA Estrés Osmótico Plantas Transgénicas Resistencia Fisiológica al Estrés Tomatoes Leaves Fruit Gene Expression Maturation Abiotic Stress Osmotic Stress Transgenic Plants Physiological Stress Resistance Ácido Abscísico Abscisic Acid Asr1 |
| url | http://hdl.handle.net/20.500.12123/4759 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107117 https://doi.org/10.1371/journal.pone.0107117 |
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