Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.)
Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence, which limits production by reducing photosynthetic capacity under specific growing conditions. Carbon for grain filling depends strongly on light interception by green leaf ar...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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PLOS
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/4756 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104379 https://doi.org/10.1371/journal.pone.0104379 |
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| author | Moschen, Sebastian Bengoa Luoni, Sofia Ailin Paniego, Norma Beatriz Hopp, Horacio Esteban Dosio, Guillermo Aníbal Adrián Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_browse | Bengoa Luoni, Sofia Ailin Dosio, Guillermo Aníbal Adrián Fernandez, Paula Del Carmen Heinz, Ruth Amelia Hopp, Horacio Esteban Moschen, Sebastian Paniego, Norma Beatriz |
| author_facet | Moschen, Sebastian Bengoa Luoni, Sofia Ailin Paniego, Norma Beatriz Hopp, Horacio Esteban Dosio, Guillermo Aníbal Adrián Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_sort | Moschen, Sebastian |
| collection | INTA Digital |
| description | Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence,
which limits production by reducing photosynthetic capacity under specific growing conditions. Carbon for grain filling
depends strongly on light interception by green leaf area, which diminishes during grain filling due to leaf senescence.
Transcription factors (TFs) regulate the progression of leaf senescence in plants and have been well explored in model
systems, but information for many agronomic crops remains limited. Here, we characterize the expression profiles of a set of
putative senescence associated genes (SAGs) identified by a candidate gene approach and sunflower microarray expression
studies. We examined a time course of sunflower leaves undergoing natural senescence and used quantitative PCR (qPCR)
to measure the expression of 11 candidate genes representing the NAC, WRKY, MYB and NF-Y TF families. In addition, we
measured physiological parameters such as chlorophyll, total soluble sugars and nitrogen content. The expression of Ha-
NAC01, Ha-NAC03, Ha-NAC04, Ha-NAC05 and Ha-MYB01 TFs increased before the remobilization rate increased and
therefore, before the appearance of the first physiological symptoms of senescence, whereas Ha-NAC02 expression
decreased. In addition, we also examined the trifurcate feed-forward pathway (involving ORE1, miR164, and ETHYLENE
INSENSITIVE 2) previously reported for Arabidopsis. We measured transcription of Ha-NAC01 (the sunflower homolog of
ORE1) and Ha-EIN2, along with the levels of miR164, in two leaves from different stem positions, and identified differences in
transcription between basal and upper leaves. Interestingly, Ha-NAC01 and Ha-EIN2 transcription profiles showed an earlier
up-regulation in upper leaves of plants close to maturity, compared with basal leaves of plants at pre-anthesis stages. These
results suggest that the H. annuus TFs characterized in this work could play important roles as potential triggers of leaf
senescence and thus can be considered putative candidate genes for senescence in sunflower. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4756 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA47562019-03-27T13:43:10Z Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) Moschen, Sebastian Bengoa Luoni, Sofia Ailin Paniego, Norma Beatriz Hopp, Horacio Esteban Dosio, Guillermo Aníbal Adrián Fernandez, Paula Del Carmen Heinz, Ruth Amelia Helianthus Annuus Hojas Arabidopsis Thaliana Clorofilas Expresión Génica Factores de Transcripción Leaves Chlorophylls Gene Expression Transcription Factors Girasol Sunflower Leaf Senescence Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence, which limits production by reducing photosynthetic capacity under specific growing conditions. Carbon for grain filling depends strongly on light interception by green leaf area, which diminishes during grain filling due to leaf senescence. Transcription factors (TFs) regulate the progression of leaf senescence in plants and have been well explored in model systems, but information for many agronomic crops remains limited. Here, we characterize the expression profiles of a set of putative senescence associated genes (SAGs) identified by a candidate gene approach and sunflower microarray expression studies. We examined a time course of sunflower leaves undergoing natural senescence and used quantitative PCR (qPCR) to measure the expression of 11 candidate genes representing the NAC, WRKY, MYB and NF-Y TF families. In addition, we measured physiological parameters such as chlorophyll, total soluble sugars and nitrogen content. The expression of Ha- NAC01, Ha-NAC03, Ha-NAC04, Ha-NAC05 and Ha-MYB01 TFs increased before the remobilization rate increased and therefore, before the appearance of the first physiological symptoms of senescence, whereas Ha-NAC02 expression decreased. In addition, we also examined the trifurcate feed-forward pathway (involving ORE1, miR164, and ETHYLENE INSENSITIVE 2) previously reported for Arabidopsis. We measured transcription of Ha-NAC01 (the sunflower homolog of ORE1) and Ha-EIN2, along with the levels of miR164, in two leaves from different stem positions, and identified differences in transcription between basal and upper leaves. Interestingly, Ha-NAC01 and Ha-EIN2 transcription profiles showed an earlier up-regulation in upper leaves of plants close to maturity, compared with basal leaves of plants at pre-anthesis stages. These results suggest that the H. annuus TFs characterized in this work could play important roles as potential triggers of leaf senescence and thus can be considered putative candidate genes for senescence in sunflower. Instituto de Biotecnología Fil: Moschen, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bengoa Luoni, Sofia Ailin. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Dosio, Guillermo Aníbal Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2019-03-27T13:14:03Z 2019-03-27T13:14:03Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4756 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104379 1932-6203 https://doi.org/10.1371/journal.pone.0104379 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 9 (8) : e104379. (2014) |
| spellingShingle | Helianthus Annuus Hojas Arabidopsis Thaliana Clorofilas Expresión Génica Factores de Transcripción Leaves Chlorophylls Gene Expression Transcription Factors Girasol Sunflower Leaf Senescence Moschen, Sebastian Bengoa Luoni, Sofia Ailin Paniego, Norma Beatriz Hopp, Horacio Esteban Dosio, Guillermo Aníbal Adrián Fernandez, Paula Del Carmen Heinz, Ruth Amelia Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title | Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_full | Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_fullStr | Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_full_unstemmed | Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_short | Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_sort | identification of candidate genes associated with leaf senescence in cultivated sunflower helianthus annuus l |
| topic | Helianthus Annuus Hojas Arabidopsis Thaliana Clorofilas Expresión Génica Factores de Transcripción Leaves Chlorophylls Gene Expression Transcription Factors Girasol Sunflower Leaf Senescence |
| url | http://hdl.handle.net/20.500.12123/4756 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104379 https://doi.org/10.1371/journal.pone.0104379 |
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