Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ c...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley; Society for Experimental Biology
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4750 https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12418 https://doi.org/10.1111/tpj.12418 |
| _version_ | 1855035377632411648 |
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| author | Bermudez Salazar, Luisa de Godoy, Fabiana Baldet, Pierre Demarco, Diego Osorio, Sonia Quadrana, Leandro Daniel Almeida de Souza, Juliana Beatriz Asis, Ramón Gibon, Yves Fernie, Alisdair R. Rossi, Maria Magdalena Carrari, Fernando |
| author_browse | Almeida de Souza, Juliana Beatriz Asis, Ramón Baldet, Pierre Bermudez Salazar, Luisa Carrari, Fernando Demarco, Diego Fernie, Alisdair R. Gibon, Yves Osorio, Sonia Quadrana, Leandro Daniel Rossi, Maria Magdalena de Godoy, Fabiana |
| author_facet | Bermudez Salazar, Luisa de Godoy, Fabiana Baldet, Pierre Demarco, Diego Osorio, Sonia Quadrana, Leandro Daniel Almeida de Souza, Juliana Beatriz Asis, Ramón Gibon, Yves Fernie, Alisdair R. Rossi, Maria Magdalena Carrari, Fernando |
| author_sort | Bermudez Salazar, Luisa |
| collection | INTA Digital |
| description | Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning
are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means
of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone
related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in
tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi
tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable
increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced
sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses
showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase,
sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These
changes would be anticipated to promote carbon export from foliar tissues. The combined results
suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the
source-sink relationships by affecting the rate of carbon translocation to fruits. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4750 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley; Society for Experimental Biology |
| publisherStr | Wiley; Society for Experimental Biology |
| record_format | dspace |
| spelling | INTA47502019-10-23T17:05:58Z Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism Bermudez Salazar, Luisa de Godoy, Fabiana Baldet, Pierre Demarco, Diego Osorio, Sonia Quadrana, Leandro Daniel Almeida de Souza, Juliana Beatriz Asis, Ramón Gibon, Yves Fernie, Alisdair R. Rossi, Maria Magdalena Carrari, Fernando Tomate Solanum Lycopersicum Azúcar Metabolismo Cloroplasto Tomatoes Sugar Metabolism Chloroplasts Small Plastidial Protein Source-sink Partitioning Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits. Instituto de Biotecnología Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil Fil: de Godoy, Fabiana. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil Fil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia Fil: Demarco, Diego. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil Fil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almeida de Souza, Juliana Beatriz. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria Fil: Rossi, Maria Magdalena. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-26T18:31:28Z 2019-03-26T18:31:28Z 2014-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4750 https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12418 1365-313X https://doi.org/10.1111/tpj.12418 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley; Society for Experimental Biology Plant journal 77 (5) : 676-687. (March 2014) |
| spellingShingle | Tomate Solanum Lycopersicum Azúcar Metabolismo Cloroplasto Tomatoes Sugar Metabolism Chloroplasts Small Plastidial Protein Source-sink Partitioning Bermudez Salazar, Luisa de Godoy, Fabiana Baldet, Pierre Demarco, Diego Osorio, Sonia Quadrana, Leandro Daniel Almeida de Souza, Juliana Beatriz Asis, Ramón Gibon, Yves Fernie, Alisdair R. Rossi, Maria Magdalena Carrari, Fernando Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism |
| title | Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism |
| title_full | Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism |
| title_fullStr | Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism |
| title_full_unstemmed | Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism |
| title_short | Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism |
| title_sort | silencing of the tomato sugar partitioning affecting protein spa modifies sink strength through a shift in leaf sugar metabolism |
| topic | Tomate Solanum Lycopersicum Azúcar Metabolismo Cloroplasto Tomatoes Sugar Metabolism Chloroplasts Small Plastidial Protein Source-sink Partitioning |
| url | http://hdl.handle.net/20.500.12123/4750 https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12418 https://doi.org/10.1111/tpj.12418 |
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