Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan
Previous studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase–telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogeneti...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Wiley
2019
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| Online Access: | https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.21836 http://hdl.handle.net/20.500.12123/4729 https://doi.org/10.1002/tox.21836 |
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| author | Pérez, Débora Jesabel Lukaszewicz, Germán Menone, Mirta Lujan Amé, María Valeria Camadro, Elsa Lucila |
| author_browse | Amé, María Valeria Camadro, Elsa Lucila Lukaszewicz, Germán Menone, Mirta Lujan Pérez, Débora Jesabel |
| author_facet | Pérez, Débora Jesabel Lukaszewicz, Germán Menone, Mirta Lujan Amé, María Valeria Camadro, Elsa Lucila |
| author_sort | Pérez, Débora Jesabel |
| collection | INTA Digital |
| description | Previous studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase–telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. For this reason, the goals of this study were to use cytogenetic biomarkers—CAAT and abnormal metaphase—and defense biomarkers such as the activity of the antioxidant enzymes—guaiacol peroxidases (POD), glutathione reductase, and microsomal and cytosolic (m‐ and c‐) glutathione‐S‐transferase (GST)—to evaluate in B. laevis effects caused by a commercial formulation of endosulfan. The frequency of CAAT was increased at 5, 10, 50, and 100 μg/L endosulfan with respect to the negative controls by 3.1, 2.5, 2.5, and 3.2‐fold, respectively while the frequency of abnormal metaphases was also increased at the same concentrations by 3.5, 2.8, 3.2, and 11.3‐fold, respectively. In addition to these aneugenic effects, other abnormalities such as C‐mitosis and chromosome clumping were observed at 10 μg/L endosulfan. On the other hand, POD induction at 0.02, 0.5, 5, and 10 μg/L and m‐GST inhibition at 0.5, 10, and 50 μg/L in plants exposed during 24 h to endosulfan were observed but all of these responses were highly variable. In conclusion, only cytogenetic biomarkers like CAAT in B. laevis can serve potentially as early warning systems to detect environmentally relevant concentrations of endosulfan in aquatic ecosystems. |
| format | Artículo |
| id | INTA4729 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA47292019-03-25T16:55:17Z Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan Pérez, Débora Jesabel Lukaszewicz, Germán Menone, Mirta Lujan Amé, María Valeria Camadro, Elsa Lucila Bidens Genotoxicidad Endosulfan Marcadores Genéticos Acaricidas Aberración Cromosómica Peroxídasas Genotoxicity Genetic Markers Acaricides Chromosome Aberrations Peroxidases Bidens laevis Marcadores Moleculares Previous studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase–telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. For this reason, the goals of this study were to use cytogenetic biomarkers—CAAT and abnormal metaphase—and defense biomarkers such as the activity of the antioxidant enzymes—guaiacol peroxidases (POD), glutathione reductase, and microsomal and cytosolic (m‐ and c‐) glutathione‐S‐transferase (GST)—to evaluate in B. laevis effects caused by a commercial formulation of endosulfan. The frequency of CAAT was increased at 5, 10, 50, and 100 μg/L endosulfan with respect to the negative controls by 3.1, 2.5, 2.5, and 3.2‐fold, respectively while the frequency of abnormal metaphases was also increased at the same concentrations by 3.5, 2.8, 3.2, and 11.3‐fold, respectively. In addition to these aneugenic effects, other abnormalities such as C‐mitosis and chromosome clumping were observed at 10 μg/L endosulfan. On the other hand, POD induction at 0.02, 0.5, 5, and 10 μg/L and m‐GST inhibition at 0.5, 10, and 50 μg/L in plants exposed during 24 h to endosulfan were observed but all of these responses were highly variable. In conclusion, only cytogenetic biomarkers like CAAT in B. laevis can serve potentially as early warning systems to detect environmentally relevant concentrations of endosulfan in aquatic ecosystems. EEA Balcarce Fil: Pérez, Débora Jesabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina Fil: Lukaszewicz, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-25T16:53:55Z 2019-03-25T16:53:55Z 2014-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.21836 http://hdl.handle.net/20.500.12123/4729 1520-4081 1522-7278 https://doi.org/10.1002/tox.21836 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Environmental Toxicology 29 (9) : 1063-1071 (September 2014) |
| spellingShingle | Bidens Genotoxicidad Endosulfan Marcadores Genéticos Acaricidas Aberración Cromosómica Peroxídasas Genotoxicity Genetic Markers Acaricides Chromosome Aberrations Peroxidases Bidens laevis Marcadores Moleculares Pérez, Débora Jesabel Lukaszewicz, Germán Menone, Mirta Lujan Amé, María Valeria Camadro, Elsa Lucila Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan |
| title | Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan |
| title_full | Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan |
| title_fullStr | Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan |
| title_full_unstemmed | Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan |
| title_short | Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan |
| title_sort | genetic and biochemical biomarkers in the macrophyte bidens laevis l exposed to a commercial formulation of endosulfan |
| topic | Bidens Genotoxicidad Endosulfan Marcadores Genéticos Acaricidas Aberración Cromosómica Peroxídasas Genotoxicity Genetic Markers Acaricides Chromosome Aberrations Peroxidases Bidens laevis Marcadores Moleculares |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.21836 http://hdl.handle.net/20.500.12123/4729 https://doi.org/10.1002/tox.21836 |
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