Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcript...
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4719 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12365 https://doi.org/10.1111/mpp.12365 |
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| author | Gonorazky, Ana Gabriela Guzzo, María Carla Abd El Haliem, Ahmed M. Joosten, Matthieu H. A. J. Laxalt, Ana Maria |
| author_browse | Abd El Haliem, Ahmed M. Gonorazky, Ana Gabriela Guzzo, María Carla Joosten, Matthieu H. A. J. Laxalt, Ana Maria |
| author_facet | Gonorazky, Ana Gabriela Guzzo, María Carla Abd El Haliem, Ahmed M. Joosten, Matthieu H. A. J. Laxalt, Ana Maria |
| author_sort | Gonorazky, Ana Gabriela |
| collection | INTA Digital |
| description | The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4719 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA47192019-03-25T13:46:16Z Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea Gonorazky, Ana Gabriela Guzzo, María Carla Abd El Haliem, Ahmed M. Joosten, Matthieu H. A. J. Laxalt, Ana Maria Tomate Tomatoes Solanum Lycopersicum Botrytis Cinerea Fosfatidilinositoles Phosphatidylinositols Cell Death Muerte Celular Jasmonic Acid Phospholipids Fosfolípidos Reactive Oxygen Species Especies de Oxígeno Reactivo Salicylic Acids Ácido Salicílico Ácido Jasmónico Necrotrophic Fungus Phospholipid Signalling The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Guzzo, Maria Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Abd El Haliem, Ahmed M. Wageningen University. Laboratory of Phytopathology; Holanda Fil: Joosten, Matthieu H. A. J. Wageningen University. Laboratory of Phytopathology; Holanda Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina 2019-03-25T13:39:46Z 2019-03-25T13:39:46Z 2016-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4719 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12365 1364-3703 (Online) https://doi.org/10.1111/mpp.12365 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Molecular plant pathology 17 (9) : 1354–1363. (December 2016) |
| spellingShingle | Tomate Tomatoes Solanum Lycopersicum Botrytis Cinerea Fosfatidilinositoles Phosphatidylinositols Cell Death Muerte Celular Jasmonic Acid Phospholipids Fosfolípidos Reactive Oxygen Species Especies de Oxígeno Reactivo Salicylic Acids Ácido Salicílico Ácido Jasmónico Necrotrophic Fungus Phospholipid Signalling Gonorazky, Ana Gabriela Guzzo, María Carla Abd El Haliem, Ahmed M. Joosten, Matthieu H. A. J. Laxalt, Ana Maria Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea |
| title | Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea |
| title_full | Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea |
| title_fullStr | Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea |
| title_full_unstemmed | Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea |
| title_short | Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea |
| title_sort | silencing of the tomato phosphatidylinositol phospholipase c2 slplc2 reduces plant susceptibility to botrytis cinerea |
| topic | Tomate Tomatoes Solanum Lycopersicum Botrytis Cinerea Fosfatidilinositoles Phosphatidylinositols Cell Death Muerte Celular Jasmonic Acid Phospholipids Fosfolípidos Reactive Oxygen Species Especies de Oxígeno Reactivo Salicylic Acids Ácido Salicílico Ácido Jasmónico Necrotrophic Fungus Phospholipid Signalling |
| url | http://hdl.handle.net/20.500.12123/4719 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12365 https://doi.org/10.1111/mpp.12365 |
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