PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans
Potato leaves infected with Phytophthora infestans produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2‐terminus of the mature PLPKI polypeptide demonstrated that PLPKI is a novel member of the potato protease inhib...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Wiley
2019
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| Subjects: | |
| Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/pbr.12129 http://hdl.handle.net/20.500.12123/4718 https://doi.org/10.1111/pbr.12129 |
| _version_ | 1855035371954372608 |
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| author | Feldman, Mariana Laura Andreu, Adriana Balbina Korgan, Samanta Lobato, Maria Candela Huarte, Marcelo Atilio Walling, Linda Lee Daleo, Gustavo Raul |
| author_browse | Andreu, Adriana Balbina Daleo, Gustavo Raul Feldman, Mariana Laura Huarte, Marcelo Atilio Korgan, Samanta Lobato, Maria Candela Walling, Linda Lee |
| author_facet | Feldman, Mariana Laura Andreu, Adriana Balbina Korgan, Samanta Lobato, Maria Candela Huarte, Marcelo Atilio Walling, Linda Lee Daleo, Gustavo Raul |
| author_sort | Feldman, Mariana Laura |
| collection | INTA Digital |
| description | Potato leaves infected with Phytophthora infestans produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2‐terminus of the mature PLPKI polypeptide demonstrated that PLPKI is a novel member of the potato protease inhibitor I family. PLPKI inhibited the activity of extracellular proteases produced by two pathogens of potato, P. infestans and Rhizoctonia solani, but was inactive against proteases secreted into the culture media by the binucleate Rhizoctonia N2, a non‐pathogenic fungus for potato. Western blot analysis showed a positive correlation between the levels of PLPKI and the degree of horizontal resistance, showing its highest accumulation in clone OKA 5632.11, which has been described as highly resistant. This correlation, together with the ability of PLPKI to completely abolish the secreted serine protease activity of P. infestans, suggests that PLPKI may have an active role in protecting potato plants from this pathogenic oomycete and that it could be used as a suitable biochemical marker to help breeders in the selection of cultivars with high degree of horizontal resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4718 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA47182019-03-25T13:02:49Z PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans Feldman, Mariana Laura Andreu, Adriana Balbina Korgan, Samanta Lobato, Maria Candela Huarte, Marcelo Atilio Walling, Linda Lee Daleo, Gustavo Raul Phytophthora infestans Papa Enfermedades de las Plantas Resistencia a la Enfermedad Serina Inhibidores de Proteinasas Potatoes Plant Diseases Disease Resistance Serine Proteinase Inhibitors Potato leaves infected with Phytophthora infestans produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2‐terminus of the mature PLPKI polypeptide demonstrated that PLPKI is a novel member of the potato protease inhibitor I family. PLPKI inhibited the activity of extracellular proteases produced by two pathogens of potato, P. infestans and Rhizoctonia solani, but was inactive against proteases secreted into the culture media by the binucleate Rhizoctonia N2, a non‐pathogenic fungus for potato. Western blot analysis showed a positive correlation between the levels of PLPKI and the degree of horizontal resistance, showing its highest accumulation in clone OKA 5632.11, which has been described as highly resistant. This correlation, together with the ability of PLPKI to completely abolish the secreted serine protease activity of P. infestans, suggests that PLPKI may have an active role in protecting potato plants from this pathogenic oomycete and that it could be used as a suitable biochemical marker to help breeders in the selection of cultivars with high degree of horizontal resistance. EEA Balcarce Fil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Korgan, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Lobato, Maria Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Walling, Linda Lee. University of California. Department of Botany and Plant Sciences; Estados Unidos Fil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina 2019-03-25T13:01:05Z 2019-03-25T13:01:05Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/10.1111/pbr.12129 http://hdl.handle.net/20.500.12123/4718 0179-9541 1439-0523 https://doi.org/10.1111/pbr.12129 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Breeding 133 (2) : 275-280 (April 2014) |
| spellingShingle | Phytophthora infestans Papa Enfermedades de las Plantas Resistencia a la Enfermedad Serina Inhibidores de Proteinasas Potatoes Plant Diseases Disease Resistance Serine Proteinase Inhibitors Feldman, Mariana Laura Andreu, Adriana Balbina Korgan, Samanta Lobato, Maria Candela Huarte, Marcelo Atilio Walling, Linda Lee Daleo, Gustavo Raul PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans |
| title | PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans |
| title_full | PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans |
| title_fullStr | PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans |
| title_full_unstemmed | PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans |
| title_short | PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans |
| title_sort | plpki a novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to phytophthora infestans |
| topic | Phytophthora infestans Papa Enfermedades de las Plantas Resistencia a la Enfermedad Serina Inhibidores de Proteinasas Potatoes Plant Diseases Disease Resistance Serine Proteinase Inhibitors |
| url | https://onlinelibrary.wiley.com/doi/10.1111/pbr.12129 http://hdl.handle.net/20.500.12123/4718 https://doi.org/10.1111/pbr.12129 |
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