Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams
This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address this question, we assessed the effect of seminal collection method on motili...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12279 http://hdl.handle.net/20.500.12123/4707 https://doi.org/10.1111/rda.12279 |
| _version_ | 1855483482033094656 |
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| author | Ledesma, Alba Manes, Jorgelina Cesari, Andreina Alberio, Ricardo Hozbor, Federico Andres |
| author_browse | Alberio, Ricardo Cesari, Andreina Hozbor, Federico Andres Ledesma, Alba Manes, Jorgelina |
| author_facet | Ledesma, Alba Manes, Jorgelina Cesari, Andreina Alberio, Ricardo Hozbor, Federico Andres |
| author_sort | Ledesma, Alba |
| collection | INTA Digital |
| description | This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address this question, we assessed the effect of seminal collection method on motility, plasma membrane integrity and functionality, mitochondrial functionality and the decondensation state of nuclear chromatin in sperm cells. Volume, pH, osmolarity, protein concentration, total protein content and protein profile using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) and 2‐D polyacrylamide electrophoresis of seminal plasma collected with artificial vagina and electroejaculation were also analysed. The main findings from this study were that ejaculates obtained with electroejaculation had (i) a higher number of spermatozoa with intact plasma membrane and functional mitochondria and (ii) a higher proportion of seminal plasma, total protein content and relative abundance of low molecular weight proteins than ejaculates obtained with artificial vagina. Five of these proteins were identified by mass spectrometry: binder of sperm 5 precursor; RSVP14; RSVP22; epididymal secretory protein E1 and clusterin. One protein spot with molecular weight of approximately 31 kDa and isoelectric point of 4.8 was only found in the seminal plasma from electroejaculation. |
| format | Artículo |
| id | INTA4707 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA47072019-03-22T14:17:05Z Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams Ledesma, Alba Manes, Jorgelina Cesari, Andreina Alberio, Ricardo Hozbor, Federico Andres Morueco Ovinos Razas (animales) Espermatozoo Semen Electroeyaculación Rams Sheep Breeds (animals) Spermatozoa Electroejaculation Carnero padre Raza Corriedale Esperma Plasma seminal This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address this question, we assessed the effect of seminal collection method on motility, plasma membrane integrity and functionality, mitochondrial functionality and the decondensation state of nuclear chromatin in sperm cells. Volume, pH, osmolarity, protein concentration, total protein content and protein profile using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) and 2‐D polyacrylamide electrophoresis of seminal plasma collected with artificial vagina and electroejaculation were also analysed. The main findings from this study were that ejaculates obtained with electroejaculation had (i) a higher number of spermatozoa with intact plasma membrane and functional mitochondria and (ii) a higher proportion of seminal plasma, total protein content and relative abundance of low molecular weight proteins than ejaculates obtained with artificial vagina. Five of these proteins were identified by mass spectrometry: binder of sperm 5 precursor; RSVP14; RSVP22; epididymal secretory protein E1 and clusterin. One protein spot with molecular weight of approximately 31 kDa and isoelectric point of 4.8 was only found in the seminal plasma from electroejaculation. EEA Balcarce Fil: Ledesma, Alba. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina 2019-03-22T14:15:26Z 2019-03-22T14:15:26Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12279 http://hdl.handle.net/20.500.12123/4707 0936-6768 1439-0531 https://doi.org/10.1111/rda.12279 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Reproduction in Domestic Animals 49 (2) : 324-332 (April 2014) |
| spellingShingle | Morueco Ovinos Razas (animales) Espermatozoo Semen Electroeyaculación Rams Sheep Breeds (animals) Spermatozoa Electroejaculation Carnero padre Raza Corriedale Esperma Plasma seminal Ledesma, Alba Manes, Jorgelina Cesari, Andreina Alberio, Ricardo Hozbor, Federico Andres Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams |
| title | Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams |
| title_full | Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams |
| title_fullStr | Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams |
| title_full_unstemmed | Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams |
| title_short | Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams |
| title_sort | electroejaculation increases low molecular weight proteins in seminal plasma modifying sperm quality in corriedale rams |
| topic | Morueco Ovinos Razas (animales) Espermatozoo Semen Electroeyaculación Rams Sheep Breeds (animals) Spermatozoa Electroejaculation Carnero padre Raza Corriedale Esperma Plasma seminal |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12279 http://hdl.handle.net/20.500.12123/4707 https://doi.org/10.1111/rda.12279 |
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