Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
Despite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properti...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4691 https://www.sciencedirect.com/science/article/pii/S0098847218317702?via%3Dihub#! https://doi.org/10.1016/j.envexpbot.2019.03.002 |
| _version_ | 1855483479098130432 |
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| author | Vezza, Mariana Elisa Luna, Dario Fernando Agostini, Elizabeth Talano, Melina Andrea |
| author_browse | Agostini, Elizabeth Luna, Dario Fernando Talano, Melina Andrea Vezza, Mariana Elisa |
| author_facet | Vezza, Mariana Elisa Luna, Dario Fernando Agostini, Elizabeth Talano, Melina Andrea |
| author_sort | Vezza, Mariana Elisa |
| collection | INTA Digital |
| description | Despite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properties and is precursor of phytochelatin (PCs) synthesis, thus its role in As-treated plants would be relevant. We proposed to assess the GSH role in the response to arsenate (AsV) and arsenite (AsIII) in soybean plants using BSO (L-buthionine-sulfoximine), an inhibitor of its synthesis. BSO treatment was efficient to reduce GSH production in roots and leaves. However, GSH reduction was lower in As/BSO-treated plants compared to control/BSO-treated plants, which suggests an As-induced activation of its synthesis. As a consequence, a decrease in PCs content and PC:As ratio in roots was observed. In addition, a reduction of antioxidant activity, including ascorbate and glutathione peroxidase activity, was detected under BSO treatment, which resulted in an increase of membrane damage and root cell death. Also, low GSH content increased inhibitory effects of As on gas exchange and PSII yield, which could explain the reduction in aerial parts biomass, mainly under AsIII-treatment. BSO-exposure also caused a significant decrease of As accumulation in roots, although increased its translocation to leaves. Our findings suggest that GSH regulated As stress tolerance in soybean plants through complex mechanisms, including redox balance, gas exchange and changes in As accumulation pattern. |
| format | Artículo |
| id | INTA4691 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA46912019-03-21T12:57:57Z Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII Vezza, Mariana Elisa Luna, Dario Fernando Agostini, Elizabeth Talano, Melina Andrea Soja Glycine Max Glutatión Reductasa Arsénico Fitoquelatos Antioxidantes Intercambio de Gases Soybeans Glutathione Reductase Arsenic Phytochelatins Antioxidants Gas Exchange Despite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properties and is precursor of phytochelatin (PCs) synthesis, thus its role in As-treated plants would be relevant. We proposed to assess the GSH role in the response to arsenate (AsV) and arsenite (AsIII) in soybean plants using BSO (L-buthionine-sulfoximine), an inhibitor of its synthesis. BSO treatment was efficient to reduce GSH production in roots and leaves. However, GSH reduction was lower in As/BSO-treated plants compared to control/BSO-treated plants, which suggests an As-induced activation of its synthesis. As a consequence, a decrease in PCs content and PC:As ratio in roots was observed. In addition, a reduction of antioxidant activity, including ascorbate and glutathione peroxidase activity, was detected under BSO treatment, which resulted in an increase of membrane damage and root cell death. Also, low GSH content increased inhibitory effects of As on gas exchange and PSII yield, which could explain the reduction in aerial parts biomass, mainly under AsIII-treatment. BSO-exposure also caused a significant decrease of As accumulation in roots, although increased its translocation to leaves. Our findings suggest that GSH regulated As stress tolerance in soybean plants through complex mechanisms, including redox balance, gas exchange and changes in As accumulation pattern. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-21T12:52:18Z 2019-03-21T12:52:18Z 2019-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4691 https://www.sciencedirect.com/science/article/pii/S0098847218317702?via%3Dihub#! 0098-8472 https://doi.org/10.1016/j.envexpbot.2019.03.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Environmental and experimental botany 162 : 272-282. (June 2019) |
| spellingShingle | Soja Glycine Max Glutatión Reductasa Arsénico Fitoquelatos Antioxidantes Intercambio de Gases Soybeans Glutathione Reductase Arsenic Phytochelatins Antioxidants Gas Exchange Vezza, Mariana Elisa Luna, Dario Fernando Agostini, Elizabeth Talano, Melina Andrea Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII |
| title | Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII |
| title_full | Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII |
| title_fullStr | Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII |
| title_full_unstemmed | Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII |
| title_short | Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII |
| title_sort | glutathione a key compound for as accumulation and tolerance in soybean plants treated with asv and asiii |
| topic | Soja Glycine Max Glutatión Reductasa Arsénico Fitoquelatos Antioxidantes Intercambio de Gases Soybeans Glutathione Reductase Arsenic Phytochelatins Antioxidants Gas Exchange |
| url | http://hdl.handle.net/20.500.12123/4691 https://www.sciencedirect.com/science/article/pii/S0098847218317702?via%3Dihub#! https://doi.org/10.1016/j.envexpbot.2019.03.002 |
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