In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4652 https://doi.org/10.1002/tox.21936 |
| _version_ | 1855483473361371136 |
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| author | Delfosse, Veronica Cecilia Tasat, Deborah Ruth Gioffre, Andrea |
| author_browse | Delfosse, Veronica Cecilia Gioffre, Andrea Tasat, Deborah Ruth |
| author_facet | Delfosse, Veronica Cecilia Tasat, Deborah Ruth Gioffre, Andrea |
| author_sort | Delfosse, Veronica Cecilia |
| collection | INTA Digital |
| description | Epidemiological studies have shown that pollution derived from industrial and vehicular
transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air
pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria
(EM) are opportunist pathogens that can affect a variety of immune compromised patients, which
impacts significantly on human morbidity and mortality. The aim of this study was to evaluate the effect of
residual oil fly ash (ROFA) pre-exposure on the pulmonary response after challenge with opportunistic
mycobacteria by means of an acute short-term in vivo experimental animal model. We exposed BALB/c
mice to ROFA and observed a significant reduction on bacterial clearance at 24 h post infection. To study
the basis of this impaired response four groups of animals were instilled with (a) saline solution (Control),
(b) ROFA (1 mg kg21 BW), (c) ROFA and EM-infected (Mycobacterium phlei, 8 3 106 CFU), and (d) EMinfected.
Animals were sacrificed 24 h postinfection and biomarkers of lung injury and proinflammatory
madiators were examined in the bronchoalveolar lavage. Our results indicate that ROFA was able to produce
an acute pulmonary injury characterized by an increase in bronchoalveolar polymorphonuclear
(PMN) cells influx and a rise in O2
2 generation. Exposure to ROFA before M. phlei infection reduced total
cell number and caused a significant decline in PMN cells recruitment (p<0.05), O2
2 generation, TNFa
(p<0.001), and IL-6 (p<0.001) levels. Hence, our results suggest that, in this animal model, the acute
short-term pre-exposure to ROFA reduces early lung response to EM infection |
| format | Artículo |
| id | INTA4652 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA46522019-03-19T13:13:04Z In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection Delfosse, Veronica Cecilia Tasat, Deborah Ruth Gioffre, Andrea Mycobacterium Respuesta Inmunológica Immune Response Pulmones Lungs Ceniza Ashes In Vivo Experimentation Mycobacterium Phlei ROFA Residual Oil Fly Ash Environmental Mycobacteria Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria (EM) are opportunist pathogens that can affect a variety of immune compromised patients, which impacts significantly on human morbidity and mortality. The aim of this study was to evaluate the effect of residual oil fly ash (ROFA) pre-exposure on the pulmonary response after challenge with opportunistic mycobacteria by means of an acute short-term in vivo experimental animal model. We exposed BALB/c mice to ROFA and observed a significant reduction on bacterial clearance at 24 h post infection. To study the basis of this impaired response four groups of animals were instilled with (a) saline solution (Control), (b) ROFA (1 mg kg21 BW), (c) ROFA and EM-infected (Mycobacterium phlei, 8 3 106 CFU), and (d) EMinfected. Animals were sacrificed 24 h postinfection and biomarkers of lung injury and proinflammatory madiators were examined in the bronchoalveolar lavage. Our results indicate that ROFA was able to produce an acute pulmonary injury characterized by an increase in bronchoalveolar polymorphonuclear (PMN) cells influx and a rise in O2 2 generation. Exposure to ROFA before M. phlei infection reduced total cell number and caused a significant decline in PMN cells recruitment (p<0.05), O2 2 generation, TNFa (p<0.001), and IL-6 (p<0.001) levels. Hence, our results suggest that, in this animal model, the acute short-term pre-exposure to ROFA reduces early lung response to EM infection Instituto de Biotecnología Fil: Delfosse, Verónica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina Fil: Gioffre, Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-03-19T13:03:56Z 2019-03-19T13:03:56Z 2015-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4652 1520-4081 https://doi.org/10.1002/tox.21936 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Environmental toxicology 30 (5) : 589-596. (May 2015) |
| spellingShingle | Mycobacterium Respuesta Inmunológica Immune Response Pulmones Lungs Ceniza Ashes In Vivo Experimentation Mycobacterium Phlei ROFA Residual Oil Fly Ash Environmental Mycobacteria Delfosse, Veronica Cecilia Tasat, Deborah Ruth Gioffre, Andrea In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| title | In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| title_full | In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| title_fullStr | In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| title_full_unstemmed | In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| title_short | In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| title_sort | in vivo short term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection |
| topic | Mycobacterium Respuesta Inmunológica Immune Response Pulmones Lungs Ceniza Ashes In Vivo Experimentation Mycobacterium Phlei ROFA Residual Oil Fly Ash Environmental Mycobacteria |
| url | http://hdl.handle.net/20.500.12123/4652 https://doi.org/10.1002/tox.21936 |
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