An insight into the sialotranscriptome and virome of Amazonian anophelines
Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and poss...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4644 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-0 https://doi.org/10.1186/s12864-019-5545-0 |
| _version_ | 1855035360189349888 |
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| author | Scarpassa, Vera Margarete Debat, Humberto Julio Alencar, Ronildo Baiatone Ferreira Saraiva, José Calvo, Eric Arcà, Bruno Ribeiro, José M. C. |
| author_browse | Alencar, Ronildo Baiatone Arcà, Bruno Calvo, Eric Debat, Humberto Julio Ferreira Saraiva, José Ribeiro, José M. C. Scarpassa, Vera Margarete |
| author_facet | Scarpassa, Vera Margarete Debat, Humberto Julio Alencar, Ronildo Baiatone Ferreira Saraiva, José Calvo, Eric Arcà, Bruno Ribeiro, José M. C. |
| author_sort | Scarpassa, Vera Margarete |
| collection | INTA Digital |
| description | Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory
substances that help the blood feeding process. The salivary polypeptides are at a fast pace of
evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their
genes leading to evasion of host immune pressure.
Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian
anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the
knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two
localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help
discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of
mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and
anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species.
Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs
(e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or
better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate
human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New
World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi.
Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become
standard within future RNAseq studies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4644 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA46442019-03-18T18:52:40Z An insight into the sialotranscriptome and virome of Amazonian anophelines Scarpassa, Vera Margarete Debat, Humberto Julio Alencar, Ronildo Baiatone Ferreira Saraiva, José Calvo, Eric Arcà, Bruno Ribeiro, José M. C. Culicidae Malaria Viroses Virosis Salivary Glands Glándulas Salivales Vectores Vectors Anopheles Transcriptome Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies. Instituto de Patología Vegetal Fil: Scarpassa, Vera Margarete .Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Alencar, Ronildo Baiatone. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil Fil: Ferreira Saraiva, José. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil Fil: Calvo, Eric. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research; Estados Unidos Fil: Arcà, Bruno. Sapienza University of Rome. Division of Parasitology. Department of Public Health and Infectious Diseases Fil: Ribeiro, José M. C. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil 2019-03-18T18:47:22Z 2019-03-18T18:47:22Z 2019 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4644 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-0 1471-2164 https://doi.org/10.1186/s12864-019-5545-0 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC genomics 20:166. (2019) |
| spellingShingle | Culicidae Malaria Viroses Virosis Salivary Glands Glándulas Salivales Vectores Vectors Anopheles Transcriptome Scarpassa, Vera Margarete Debat, Humberto Julio Alencar, Ronildo Baiatone Ferreira Saraiva, José Calvo, Eric Arcà, Bruno Ribeiro, José M. C. An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_full | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_fullStr | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_full_unstemmed | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_short | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_sort | insight into the sialotranscriptome and virome of amazonian anophelines |
| topic | Culicidae Malaria Viroses Virosis Salivary Glands Glándulas Salivales Vectores Vectors Anopheles Transcriptome |
| url | http://hdl.handle.net/20.500.12123/4644 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-0 https://doi.org/10.1186/s12864-019-5545-0 |
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