Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and im...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4635 https://www.sciencedirect.com/science/article/pii/S0034528818302595?via%3Dihub#! https://doi.org/10.1016/j.rvsc.2018.09.009 |
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| author | Colombatti Olivieri, Maria Alejandra Moyano, Roberto Damian Traveria, Gabriel Eduardo Alvarado Pinedo, Maria Fiorella Mon, Maria Laura Gravisaco, María José Delgado, Fernando Oscar Santangelo, María De La Paz Romano, Maria Isabel |
| author_browse | Alvarado Pinedo, Maria Fiorella Colombatti Olivieri, Maria Alejandra Delgado, Fernando Oscar Gravisaco, María José Mon, Maria Laura Moyano, Roberto Damian Romano, Maria Isabel Santangelo, María De La Paz Traveria, Gabriel Eduardo |
| author_facet | Colombatti Olivieri, Maria Alejandra Moyano, Roberto Damian Traveria, Gabriel Eduardo Alvarado Pinedo, Maria Fiorella Mon, Maria Laura Gravisaco, María José Delgado, Fernando Oscar Santangelo, María De La Paz Romano, Maria Isabel |
| author_sort | Colombatti Olivieri, Maria Alejandra |
| collection | INTA Digital |
| description | Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian strains of Map with different genotype in a murine model. Four local isolates (Cattle type) with different genotypes (analyzed by MIRU-VNTR and SSRs) were selected and evaluated in a virulence assay in BALB/c mice. This assay allowed us to differentiate virulent and low-virulence Map strains. The less virulent strains (1543/481 and A162) failed to induce a significant production of the proinflammatory cytokine IFNg, whereas the virulent strain 6611 established infection along with a proinflammatory immune response. On the other hand, the virulent strain 1347/498 was efficient in establishing a persistent infection, but failed to promote an important Th1 response compared with 6611 at the evaluated time. We selected the low-virulence strain 1543/498 as a live vaccine and the virulent strain 6611 as a live and inactivated vaccine in a protection assay in mice. Strain 1543/481 failed to protect the animals from challenge, whereas strain 6611, in its live and inactivated form, significantly reduced the CFUs count in the infected mice, although they had different immunological response profiles. The inactivated virulent strain 6611 is a potential vaccine candidate against paratuberculosis to be tested in cattle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4635 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA46352019-09-12T15:07:03Z Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model Colombatti Olivieri, Maria Alejandra Moyano, Roberto Damian Traveria, Gabriel Eduardo Alvarado Pinedo, Maria Fiorella Mon, Maria Laura Gravisaco, María José Delgado, Fernando Oscar Santangelo, María De La Paz Romano, Maria Isabel Mycobacterium Avium Subsp. Paratuberculosis Genotipos Virulencia Vacuna Respuesta Inmunológica Genotypes Virulence Vaccines Immune Response Murine Model Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian strains of Map with different genotype in a murine model. Four local isolates (Cattle type) with different genotypes (analyzed by MIRU-VNTR and SSRs) were selected and evaluated in a virulence assay in BALB/c mice. This assay allowed us to differentiate virulent and low-virulence Map strains. The less virulent strains (1543/481 and A162) failed to induce a significant production of the proinflammatory cytokine IFNg, whereas the virulent strain 6611 established infection along with a proinflammatory immune response. On the other hand, the virulent strain 1347/498 was efficient in establishing a persistent infection, but failed to promote an important Th1 response compared with 6611 at the evaluated time. We selected the low-virulence strain 1543/498 as a live vaccine and the virulent strain 6611 as a live and inactivated vaccine in a protection assay in mice. Strain 1543/481 failed to protect the animals from challenge, whereas strain 6611, in its live and inactivated form, significantly reduced the CFUs count in the infected mice, although they had different immunological response profiles. The inactivated virulent strain 6611 is a potential vaccine candidate against paratuberculosis to be tested in cattle. Instituto de Biotecnología Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Traveria, Gabriel Eduardo. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina Fil: Alvarado Pinedo, María Fiorella. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-18T14:03:18Z 2019-03-18T14:03:18Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4635 https://www.sciencedirect.com/science/article/pii/S0034528818302595?via%3Dihub#! 0034-5288 https://doi.org/10.1016/j.rvsc.2018.09.009 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Research in veterinary science 121 : 4-11. (December 2018) |
| spellingShingle | Mycobacterium Avium Subsp. Paratuberculosis Genotipos Virulencia Vacuna Respuesta Inmunológica Genotypes Virulence Vaccines Immune Response Murine Model Colombatti Olivieri, Maria Alejandra Moyano, Roberto Damian Traveria, Gabriel Eduardo Alvarado Pinedo, Maria Fiorella Mon, Maria Laura Gravisaco, María José Delgado, Fernando Oscar Santangelo, María De La Paz Romano, Maria Isabel Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model |
| title | Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model |
| title_full | Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model |
| title_fullStr | Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model |
| title_full_unstemmed | Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model |
| title_short | Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model |
| title_sort | protection efficacy of argentinian isolates of mycobacterium avium subsp paratuberculosis with different genotypes and virulence in a murine model |
| topic | Mycobacterium Avium Subsp. Paratuberculosis Genotipos Virulencia Vacuna Respuesta Inmunológica Genotypes Virulence Vaccines Immune Response Murine Model |
| url | http://hdl.handle.net/20.500.12123/4635 https://www.sciencedirect.com/science/article/pii/S0034528818302595?via%3Dihub#! https://doi.org/10.1016/j.rvsc.2018.09.009 |
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