A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass
Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain The...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
SpringerOpen
2019
|
| Subjects: | |
| Online Access: | https://amb-express.springeropen.com/articles/10.1186/s13568-019-0748-y http://hdl.handle.net/20.500.12123/4634 https://doi.org/10.1186/s13568-019-0748-y |
| _version_ | 1855483470151680000 |
|---|---|
| author | Navas, Laura Emilce Martinez, Fernando Taverna, María Eugenia Fetherolf, Morgan M. Eltis, Lindsay D. Nicolau, Veronica Viviana Estenoz, Diana Alejandra Campos, Eleonora Benintende, Graciela Beatriz Berretta, Marcelo Facundo |
| author_browse | Benintende, Graciela Beatriz Berretta, Marcelo Facundo Campos, Eleonora Eltis, Lindsay D. Estenoz, Diana Alejandra Fetherolf, Morgan M. Martinez, Fernando Navas, Laura Emilce Nicolau, Veronica Viviana Taverna, María Eugenia |
| author_facet | Navas, Laura Emilce Martinez, Fernando Taverna, María Eugenia Fetherolf, Morgan M. Eltis, Lindsay D. Nicolau, Veronica Viviana Estenoz, Diana Alejandra Campos, Eleonora Benintende, Graciela Beatriz Berretta, Marcelo Facundo |
| author_sort | Navas, Laura Emilce |
| collection | INTA Digital |
| description | Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain Thermus sp. 2.9 and investigate its capacity to delignify lignocellulosic biomass. The purified enzyme displayed a blue color typical of laccases, showed strict copper dependence and retained 80% of its activity after 16 h at 70 °C. At 60 °C, the enzyme oxidized 2,2′-azino-di-(3-ethylbenzthiazoline sulfonate) (ABTS) and 2,6-dimethoxyphenol (DMP) at optimal pH of 5 and 6, respectively. LAC_2.9 had higher substrate specificity (kcat/KM) for DMP with a calculated value that accounts for one of the highest reported for laccases. Further, the enzyme oxidized a phenolic lignin model dimer. The incubation of steam-exploded eucalyptus biomass with LAC_2.9 and 1-hydroxybenzotriazole (HBT) as mediator changed the structural properties of the lignocellulose as evidenced by Fourier transform infrared (FTIR) spectroscopy and thermo-gravimetric analysis (TGA). However, this did not increase the yield of sugars released by enzymatic saccharification. In conclusion, LAC_2.9 is a thermostable laccase with potential application in the delignification of lignocellulosic biomass. |
| format | Artículo |
| id | INTA4634 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | SpringerOpen |
| publisherStr | SpringerOpen |
| record_format | dspace |
| spelling | INTA46342020-01-07T15:40:48Z A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass Navas, Laura Emilce Martinez, Fernando Taverna, María Eugenia Fetherolf, Morgan M. Eltis, Lindsay D. Nicolau, Veronica Viviana Estenoz, Diana Alejandra Campos, Eleonora Benintende, Graciela Beatriz Berretta, Marcelo Facundo Lacasa Oxidorreductasas Eucalyptus Biomasa Delignificación Potencial Redox Laccase Oxidoreductases Biomass Delignification Redox Potential Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain Thermus sp. 2.9 and investigate its capacity to delignify lignocellulosic biomass. The purified enzyme displayed a blue color typical of laccases, showed strict copper dependence and retained 80% of its activity after 16 h at 70 °C. At 60 °C, the enzyme oxidized 2,2′-azino-di-(3-ethylbenzthiazoline sulfonate) (ABTS) and 2,6-dimethoxyphenol (DMP) at optimal pH of 5 and 6, respectively. LAC_2.9 had higher substrate specificity (kcat/KM) for DMP with a calculated value that accounts for one of the highest reported for laccases. Further, the enzyme oxidized a phenolic lignin model dimer. The incubation of steam-exploded eucalyptus biomass with LAC_2.9 and 1-hydroxybenzotriazole (HBT) as mediator changed the structural properties of the lignocellulose as evidenced by Fourier transform infrared (FTIR) spectroscopy and thermo-gravimetric analysis (TGA). However, this did not increase the yield of sugars released by enzymatic saccharification. In conclusion, LAC_2.9 is a thermostable laccase with potential application in the delignification of lignocellulosic biomass. Instituto de Microbiología y Zoología Agrícola Fil: Navas, Laura Emilce. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Fil: Taverna, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Tecnológica Nacional. Facultad Regional San Francisco; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fetherolf, Morgan M. University of British Columbia. Department of Microbiology & Immunology; Canadá Fil: Eltis, Lindsay D. University of British Columbia. Department of Microbiology & Immunology; Canadá Fil: Nicolau, Veronica Viviana. Universidad Tecnológica Nacional. Facultad Regional San Francisco; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Estenoz, Diana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-18T13:36:06Z 2019-03-18T13:36:06Z 2019-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://amb-express.springeropen.com/articles/10.1186/s13568-019-0748-y http://hdl.handle.net/20.500.12123/4634 2191-0855 https://doi.org/10.1186/s13568-019-0748-y eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf SpringerOpen AMB Express 9 : 24 (February 2019) |
| spellingShingle | Lacasa Oxidorreductasas Eucalyptus Biomasa Delignificación Potencial Redox Laccase Oxidoreductases Biomass Delignification Redox Potential Navas, Laura Emilce Martinez, Fernando Taverna, María Eugenia Fetherolf, Morgan M. Eltis, Lindsay D. Nicolau, Veronica Viviana Estenoz, Diana Alejandra Campos, Eleonora Benintende, Graciela Beatriz Berretta, Marcelo Facundo A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass |
| title | A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass |
| title_full | A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass |
| title_fullStr | A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass |
| title_full_unstemmed | A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass |
| title_short | A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass |
| title_sort | thermostable laccase from thermus sp 2 9 and its potential for delignification of eucalyptus biomass |
| topic | Lacasa Oxidorreductasas Eucalyptus Biomasa Delignificación Potencial Redox Laccase Oxidoreductases Biomass Delignification Redox Potential |
| url | https://amb-express.springeropen.com/articles/10.1186/s13568-019-0748-y http://hdl.handle.net/20.500.12123/4634 https://doi.org/10.1186/s13568-019-0748-y |
| work_keys_str_mv | AT navaslauraemilce athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT martinezfernando athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT tavernamariaeugenia athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT fetherolfmorganm athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT eltislindsayd athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT nicolauveronicaviviana athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT estenozdianaalejandra athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT camposeleonora athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT benintendegracielabeatriz athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT berrettamarcelofacundo athermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT navaslauraemilce thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT martinezfernando thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT tavernamariaeugenia thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT fetherolfmorganm thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT eltislindsayd thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT nicolauveronicaviviana thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT estenozdianaalejandra thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT camposeleonora thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT benintendegracielabeatriz thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass AT berrettamarcelofacundo thermostablelaccasefromthermussp29anditspotentialfordelignificationofeucalyptusbiomass |