A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
Citrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains B and C. The B strain is less pathogenic than the A strain, but pro...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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British Society for Plant Pathology
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/4628 https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12361 https://doi.org/10.1111/ppa.12361 |
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| author | Gochez, Alberto Martin Minsavage, Gerald V. Potnis, Neha Canteros, Blanca Isabel Stall, Robert E. Jones, Jeffrey B. |
| author_browse | Canteros, Blanca Isabel Gochez, Alberto Martin Jones, Jeffrey B. Minsavage, Gerald V. Potnis, Neha Stall, Robert E. |
| author_facet | Gochez, Alberto Martin Minsavage, Gerald V. Potnis, Neha Canteros, Blanca Isabel Stall, Robert E. Jones, Jeffrey B. |
| author_sort | Gochez, Alberto Martin |
| collection | INTA Digital |
| description | Citrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen
where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains
B and C. The B strain is less pathogenic than the A strain, but produces symptoms in all citrus species. The C-type cankers
only infect Key lime (Citrus aurantifolia) and produce a hypersensitive reaction (HR) in grapefruit (Citrus paradisi)
leaves. An avirulence gene, avrGf2, was identified in a C strain that was responsible for the HR in grapefruit. AvrGf2
is a member of XopAG effector family and shares 45% identity at amino acid level with another member of the same
family, AvrGf1 from strain Xc-Aw, which was previously shown to elicit an HR in grapefruit. AvrGf2 shares sequence
identity with other XopAG effectors present in Xanthomonas vasculorum, Xanthomonas campestris pv. musacearum
and Pseudomonas syringae pv. tomato. Mutagenesis of avrGf2 in C strain resulted in a compatible reaction in grapefruit.
There was no observable effect on virulence when Xc-A transconjugants containing either avirulence gene were
inoculated on Key lime. Expression of avrGf1 or avrGf2 in Xc-A resulted in a similar phenotype following infiltration
into grapefruit leaves, although the avrGf2 transconjugant elicited a faster HR and lower populations than the transconjugant
containing avrGf1. Also, it was shown that all Xfa-B strains tested contain a transposon in avrGf2 that helps
to explain the differences in host range between B and C strains. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4628 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | British Society for Plant Pathology |
| publisherStr | British Society for Plant Pathology |
| record_format | dspace |
| spelling | INTA46282019-03-15T17:36:45Z A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range Gochez, Alberto Martin Minsavage, Gerald V. Potnis, Neha Canteros, Blanca Isabel Stall, Robert E. Jones, Jeffrey B. Avirulence Hypersensitivity Disease Resistance Avirulencia Resistencia a la Enfermedad Citrus Xanthomonas Xanthomonas Fuscans Hipersensibilidad Citrus Canker XopAG Citrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains B and C. The B strain is less pathogenic than the A strain, but produces symptoms in all citrus species. The C-type cankers only infect Key lime (Citrus aurantifolia) and produce a hypersensitive reaction (HR) in grapefruit (Citrus paradisi) leaves. An avirulence gene, avrGf2, was identified in a C strain that was responsible for the HR in grapefruit. AvrGf2 is a member of XopAG effector family and shares 45% identity at amino acid level with another member of the same family, AvrGf1 from strain Xc-Aw, which was previously shown to elicit an HR in grapefruit. AvrGf2 shares sequence identity with other XopAG effectors present in Xanthomonas vasculorum, Xanthomonas campestris pv. musacearum and Pseudomonas syringae pv. tomato. Mutagenesis of avrGf2 in C strain resulted in a compatible reaction in grapefruit. There was no observable effect on virulence when Xc-A transconjugants containing either avirulence gene were inoculated on Key lime. Expression of avrGf1 or avrGf2 in Xc-A resulted in a similar phenotype following infiltration into grapefruit leaves, although the avrGf2 transconjugant elicited a faster HR and lower populations than the transconjugant containing avrGf1. Also, it was shown that all Xfa-B strains tested contain a transposon in avrGf2 that helps to explain the differences in host range between B and C strains. EEA Bella Vista Fil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina. University of Florida. Department of Plant Pathology ; Estados Unidos Fil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos Fil: Potnis, Neha. University of Florida. Department of Plant Pathology; Estados Unidos Fil: Canteros, Blanca Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina Fil: Stall, Robert E. University of Florida. Department of Plant Pathology; Estados Unidos Fil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos 2019-03-15T17:29:31Z 2019-03-15T17:29:31Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4628 https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12361 1365-3059 (Online) https://doi.org/10.1111/ppa.12361 eng info:eu-repo/semantics/restrictedAccess application/pdf British Society for Plant Pathology Plant pathology 64 : 1207–1214. (2015) |
| spellingShingle | Avirulence Hypersensitivity Disease Resistance Avirulencia Resistencia a la Enfermedad Citrus Xanthomonas Xanthomonas Fuscans Hipersensibilidad Citrus Canker XopAG Gochez, Alberto Martin Minsavage, Gerald V. Potnis, Neha Canteros, Blanca Isabel Stall, Robert E. Jones, Jeffrey B. A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range |
| title | A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range |
| title_full | A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range |
| title_fullStr | A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range |
| title_full_unstemmed | A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range |
| title_short | A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range |
| title_sort | functional xopag homologue in xanthomonas fuscans pv aurantifolii strain c limits host range |
| topic | Avirulence Hypersensitivity Disease Resistance Avirulencia Resistencia a la Enfermedad Citrus Xanthomonas Xanthomonas Fuscans Hipersensibilidad Citrus Canker XopAG |
| url | http://hdl.handle.net/20.500.12123/4628 https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12361 https://doi.org/10.1111/ppa.12361 |
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