Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV...

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Autores principales: Bok, Marina, Miño, Samuel, Rodriguez, Daniela, Badaracco, Alejandra, Nuñes, I., Souza, Sibele P., Bilbao, G., Louge Uriarte, Enrique Leopoldo, Galarza, Roxana Ivon, Vega, Celina Guadalupe, Odeon, Anselmo Carlos, Saif, LInda J., Parreño, Gladys Viviana
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/4591
https://www.sciencedirect.com/science/article/pii/S0378113515300523?via%3Dihub
https://doi.org/10.1016/j.vetmic.2015.10.017
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author Bok, Marina
Miño, Samuel
Rodriguez, Daniela
Badaracco, Alejandra
Nuñes, I.
Souza, Sibele P.
Bilbao, G.
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Vega, Celina Guadalupe
Odeon, Anselmo Carlos
Saif, LInda J.
Parreño, Gladys Viviana
author_browse Badaracco, Alejandra
Bilbao, G.
Bok, Marina
Galarza, Roxana Ivon
Louge Uriarte, Enrique Leopoldo
Miño, Samuel
Nuñes, I.
Odeon, Anselmo Carlos
Parreño, Gladys Viviana
Rodriguez, Daniela
Saif, LInda J.
Souza, Sibele P.
Vega, Celina Guadalupe
author_facet Bok, Marina
Miño, Samuel
Rodriguez, Daniela
Badaracco, Alejandra
Nuñes, I.
Souza, Sibele P.
Bilbao, G.
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Vega, Celina Guadalupe
Odeon, Anselmo Carlos
Saif, LInda J.
Parreño, Gladys Viviana
author_sort Bok, Marina
collection INTA Digital
description Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle
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spelling INTA45912019-04-05T12:06:11Z Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010 Bok, Marina Miño, Samuel Rodriguez, Daniela Badaracco, Alejandra Nuñes, I. Souza, Sibele P. Bilbao, G. Louge Uriarte, Enrique Leopoldo Galarza, Roxana Ivon Vega, Celina Guadalupe Odeon, Anselmo Carlos Saif, LInda J. Parreño, Gladys Viviana Coronavirus Ganado Bovino Diarrea Viral Bovina Cattle Bovine Viral Diarrhoea Argentina Neonatal Calf Diarrhea Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle Instituto de Virología Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Chinese Academy of Agriculture Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Rodriguez, Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Nuñes, I. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Souza, Sibele P. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Bilbao, G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; Argentina Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional del Centro de la provincia de Buenos Aires. Facultad Ciencias Veterinarias; Argentina Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Odeon, Anselmo Carlos. Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; Argentina Fil: Saif, LInda J. Ohio State University. Food Animal Health Research Program; Estados Unidos Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2019-03-13T14:46:00Z 2019-03-13T14:46:00Z 2015-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4591 https://www.sciencedirect.com/science/article/pii/S0378113515300523?via%3Dihub 0378-1135 https://doi.org/10.1016/j.vetmic.2015.10.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary microbiology 181 (3–4) : 221-229. (31 December 2015)
spellingShingle Coronavirus
Ganado Bovino
Diarrea Viral Bovina
Cattle
Bovine Viral Diarrhoea
Argentina
Neonatal Calf Diarrhea
Bok, Marina
Miño, Samuel
Rodriguez, Daniela
Badaracco, Alejandra
Nuñes, I.
Souza, Sibele P.
Bilbao, G.
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Vega, Celina Guadalupe
Odeon, Anselmo Carlos
Saif, LInda J.
Parreño, Gladys Viviana
Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_full Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_fullStr Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_full_unstemmed Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_short Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_sort molecular and antigenic characterization of bovine coronavirus circulating in argentinean cattle during 1994 2010
topic Coronavirus
Ganado Bovino
Diarrea Viral Bovina
Cattle
Bovine Viral Diarrhoea
Argentina
Neonatal Calf Diarrhea
url http://hdl.handle.net/20.500.12123/4591
https://www.sciencedirect.com/science/article/pii/S0378113515300523?via%3Dihub
https://doi.org/10.1016/j.vetmic.2015.10.017
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