Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimina...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4587 https://doi.org/10.1016/j.meegid.2014.12.027 |
| _version_ | 1855035350243606528 |
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| author | Guillemi, Eliana Carolina Ruybal, Paula Lia, Veronica Viviana Gonzalez, Sergio Alberto Lew, Sergio Eduardo Zimmer, Patricia Andrea Lopez Arias, Ludmila Sol Rodríguez, José Luis Rodriguez, Sonia Y. Frutos, Roger Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
| author_browse | Farber, Marisa Diana Frutos, Roger Gonzalez, Sergio Alberto Guillemi, Eliana Carolina Lew, Sergio Eduardo Lia, Veronica Viviana Lopez Arias, Ludmila Sol Rodriguez, Sonia Y. Rodríguez, José Luis Ruybal, Paula Wilkowsky, Silvina Elizabeth Zimmer, Patricia Andrea |
| author_facet | Guillemi, Eliana Carolina Ruybal, Paula Lia, Veronica Viviana Gonzalez, Sergio Alberto Lew, Sergio Eduardo Zimmer, Patricia Andrea Lopez Arias, Ludmila Sol Rodríguez, José Luis Rodriguez, Sonia Y. Frutos, Roger Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
| author_sort | Guillemi, Eliana Carolina |
| collection | INTA Digital |
| description | Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. Here we report the development of an MLST scheme based on the study of seven genes: dnaA, ftsZ, groEl, lipA, recA, secY and sucB. Five annotated genomes (Saint Maries, Florida, Mississippi, Puerto Rico and Virginia) and 53 bovine blood samples from different world regions were analyzed. High nucleotide diversity and a large proportion of synonymous substitutions, indicative of negative selection resulted from DnaSP 5.00.02 package application. Recombination events were detected in almost all genes, this evidence together with the coexistence of more than one A. marginale strain in the same sample might suggest the superinfection phenomena as a potential source of variation. The allelic profile analysis performed through GoeBURST shown two main CC that did not support geography. In addition, the AMOVA test confirmed the occurrence of at least two main genetically divergent groups. The composition of the emergent groups reflected the impact of both historical and environmental traits on A. marginale population structure. Finally, a web-based platform “Galaxy MLST-Pipeline” was developed to automate DNA sequence editing and data analysis that together with the Data Base are freely available to users.
The A. marginale MLST scheme developed here is a valuable tool with a high discrimination power, besides PCR based strategies are still the better choice for epidemiological intracellular pathogens studies. Finally, the allelic profile describe herein would contribute to uncover the mechanisms in how intracellular pathogens challenge virulence paradigm. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4587 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45872024-04-18T15:00:06Z Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time Guillemi, Eliana Carolina Ruybal, Paula Lia, Veronica Viviana Gonzalez, Sergio Alberto Lew, Sergio Eduardo Zimmer, Patricia Andrea Lopez Arias, Ludmila Sol Rodríguez, José Luis Rodriguez, Sonia Y. Frutos, Roger Wilkowsky, Silvina Elizabeth Farber, Marisa Diana Enfermedades de los Animales Anaplasma Marginale Epidemiología Enfermedades Transmitidas por Garrapatas Anaplasmosis Ganado Bovino Animal Diseases Epidemiology Tickborne Diseases Cattle Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. Here we report the development of an MLST scheme based on the study of seven genes: dnaA, ftsZ, groEl, lipA, recA, secY and sucB. Five annotated genomes (Saint Maries, Florida, Mississippi, Puerto Rico and Virginia) and 53 bovine blood samples from different world regions were analyzed. High nucleotide diversity and a large proportion of synonymous substitutions, indicative of negative selection resulted from DnaSP 5.00.02 package application. Recombination events were detected in almost all genes, this evidence together with the coexistence of more than one A. marginale strain in the same sample might suggest the superinfection phenomena as a potential source of variation. The allelic profile analysis performed through GoeBURST shown two main CC that did not support geography. In addition, the AMOVA test confirmed the occurrence of at least two main genetically divergent groups. The composition of the emergent groups reflected the impact of both historical and environmental traits on A. marginale population structure. Finally, a web-based platform “Galaxy MLST-Pipeline” was developed to automate DNA sequence editing and data analysis that together with the Data Base are freely available to users. The A. marginale MLST scheme developed here is a valuable tool with a high discrimination power, besides PCR based strategies are still the better choice for epidemiological intracellular pathogens studies. Finally, the allelic profile describe herein would contribute to uncover the mechanisms in how intracellular pathogens challenge virulence paradigm. Instituto de Biotecnología Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lew, Sergio Eduardo. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina Fil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rodriguez, Jose L.. Corporación Colombiana de Investigación Agropecuaria; Colombia Fil: Rodriguez, Sonia Y.. Corporación Colombiana de Investigación Agropecuaria; Colombia Fil: Frutos, Roger. Centre de Coopération Internationale en Recherche Agronomique pour le Développerment; Francia Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-03-13T13:12:28Z 2019-03-13T13:12:28Z 2015-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4587 1567-1348 https://doi.org/10.1016/j.meegid.2014.12.027 eng info:eu-repo/semantics/openAccess application/pdf Elsevier Infection, Genetics and Evolution 30 : 186-194 (March 2015) |
| spellingShingle | Enfermedades de los Animales Anaplasma Marginale Epidemiología Enfermedades Transmitidas por Garrapatas Anaplasmosis Ganado Bovino Animal Diseases Epidemiology Tickborne Diseases Cattle Guillemi, Eliana Carolina Ruybal, Paula Lia, Veronica Viviana Gonzalez, Sergio Alberto Lew, Sergio Eduardo Zimmer, Patricia Andrea Lopez Arias, Ludmila Sol Rodríguez, José Luis Rodriguez, Sonia Y. Frutos, Roger Wilkowsky, Silvina Elizabeth Farber, Marisa Diana Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time |
| title | Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time |
| title_full | Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time |
| title_fullStr | Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time |
| title_full_unstemmed | Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time |
| title_short | Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time |
| title_sort | development of a multilocus sequence typing scheme for the study of anaplasma marginale population structure over space and time |
| topic | Enfermedades de los Animales Anaplasma Marginale Epidemiología Enfermedades Transmitidas por Garrapatas Anaplasmosis Ganado Bovino Animal Diseases Epidemiology Tickborne Diseases Cattle |
| url | http://hdl.handle.net/20.500.12123/4587 https://doi.org/10.1016/j.meegid.2014.12.027 |
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