Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis
Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity under the exposure to protein purified derivative tuberculin (PPD), a complex and...
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
|
| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S1472979215207688 http://hdl.handle.net/20.500.12123/4585 https://doi.org/10.1016/j.tube.2015.07.009 |
| _version_ | 1855035349868216320 |
|---|---|
| author | Eirin, Maria Emilia Macías, Analía Magnano, Gabriel Morsella, Claudia Graciela Mendez, Laura Luján Blanco, Federico Carlos Bianco, María Veronica Severina, Walter Alito, Alicia Elsa Pando, Maria de los Angeles Singh, Mahavir G. Spallek, Ralph Paolicchi, Fernando Bigi, Fabiana Cataldi, Angel Adrian |
| author_browse | Alito, Alicia Elsa Bianco, María Veronica Bigi, Fabiana Blanco, Federico Carlos Cataldi, Angel Adrian Eirin, Maria Emilia Macías, Analía Magnano, Gabriel Mendez, Laura Luján Morsella, Claudia Graciela Pando, Maria de los Angeles Paolicchi, Fernando Severina, Walter Singh, Mahavir G. Spallek, Ralph |
| author_facet | Eirin, Maria Emilia Macías, Analía Magnano, Gabriel Morsella, Claudia Graciela Mendez, Laura Luján Blanco, Federico Carlos Bianco, María Veronica Severina, Walter Alito, Alicia Elsa Pando, Maria de los Angeles Singh, Mahavir G. Spallek, Ralph Paolicchi, Fernando Bigi, Fabiana Cataldi, Angel Adrian |
| author_sort | Eirin, Maria Emilia |
| collection | INTA Digital |
| description | Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity under the exposure to protein purified derivative tuberculin (PPD), a complex and poorly characterized reagent. The cross-reactivity to non-tuberculous mycobacterium species (false-positive results) has been crucial to develop a more proper antigen. In the present study, we selected six M. bovis Open Reading Frames (Mb1992, Mb2031c, Mb2319, Mb2843c, Mb2845c and Mb3212c) by in-silico analysis and evaluated them in experimental and natural infection; none of these antigens had been previously assessed as diagnostic antigens for bTB. The reactivity performance was tested in animals with both positive and negative Tuberculin Skin Test (TST) results as well as in cattle infected with Mycobacterium avium subesp. paratuberculosis (MAP). The six recombinant antigens individually induced an IFN-γ response, with overall responder frequency ranging from 18.3 to 31%. Mb2845c was the most valuable antigen with the potential to discriminate TST-positive cattle from either TST-negative or MAP infected animals. Mb2845c showed similar performance to that observed with ESAT-6 and PPD-B among TST and MTC specific-PCR positive animals, although this result needs to be proven in further studies with a higher sample size. Our data confirm the feacibility to implement bioinformatic screening tools and suggest Mb2845c as a potential diagnostic antigen to be tested in protein cocktails to evaluate their contribution to bTB diagnosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4585 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45852019-03-13T12:43:25Z Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis Eirin, Maria Emilia Macías, Analía Magnano, Gabriel Morsella, Claudia Graciela Mendez, Laura Luján Blanco, Federico Carlos Bianco, María Veronica Severina, Walter Alito, Alicia Elsa Pando, Maria de los Angeles Singh, Mahavir G. Spallek, Ralph Paolicchi, Fernando Bigi, Fabiana Cataldi, Angel Adrian Enfermedades de los Animales Ganado Bovino Tuberculosis Infeccion por Mycobacterium bovis Mycobacterium Bovis Antígenos Interferonas Diagnóstico Animal Diseases Cattle Mycobacterium bovis Infections Antigens Interferons Diagnosis Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity under the exposure to protein purified derivative tuberculin (PPD), a complex and poorly characterized reagent. The cross-reactivity to non-tuberculous mycobacterium species (false-positive results) has been crucial to develop a more proper antigen. In the present study, we selected six M. bovis Open Reading Frames (Mb1992, Mb2031c, Mb2319, Mb2843c, Mb2845c and Mb3212c) by in-silico analysis and evaluated them in experimental and natural infection; none of these antigens had been previously assessed as diagnostic antigens for bTB. The reactivity performance was tested in animals with both positive and negative Tuberculin Skin Test (TST) results as well as in cattle infected with Mycobacterium avium subesp. paratuberculosis (MAP). The six recombinant antigens individually induced an IFN-γ response, with overall responder frequency ranging from 18.3 to 31%. Mb2845c was the most valuable antigen with the potential to discriminate TST-positive cattle from either TST-negative or MAP infected animals. Mb2845c showed similar performance to that observed with ESAT-6 and PPD-B among TST and MTC specific-PCR positive animals, although this result needs to be proven in further studies with a higher sample size. Our data confirm the feacibility to implement bioinformatic screening tools and suggest Mb2845c as a potential diagnostic antigen to be tested in protein cocktails to evaluate their contribution to bTB diagnosis. Instituto de Biotecnología Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Severina, Walter. Actividad privada; Argentina Fil: Alito, Alicia Elsa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pando, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Singh, Mahavir G. LIONEX GmbH; Alemania Fil: Spallek, Ralph. LIONEX GmbH; Alemania Fil: Paoliochi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-03-13T12:41:29Z 2019-03-13T12:41:29Z 2015-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1472979215207688 http://hdl.handle.net/20.500.12123/4585 1472-9792 1873-281X https://doi.org/10.1016/j.tube.2015.07.009 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 95 (6) : 795-801 (December 2015) |
| spellingShingle | Enfermedades de los Animales Ganado Bovino Tuberculosis Infeccion por Mycobacterium bovis Mycobacterium Bovis Antígenos Interferonas Diagnóstico Animal Diseases Cattle Mycobacterium bovis Infections Antigens Interferons Diagnosis Eirin, Maria Emilia Macías, Analía Magnano, Gabriel Morsella, Claudia Graciela Mendez, Laura Luján Blanco, Federico Carlos Bianco, María Veronica Severina, Walter Alito, Alicia Elsa Pando, Maria de los Angeles Singh, Mahavir G. Spallek, Ralph Paolicchi, Fernando Bigi, Fabiana Cataldi, Angel Adrian Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| title | Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| title_full | Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| title_fullStr | Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| title_full_unstemmed | Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| title_short | Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| title_sort | identification and evaluation of new mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis |
| topic | Enfermedades de los Animales Ganado Bovino Tuberculosis Infeccion por Mycobacterium bovis Mycobacterium Bovis Antígenos Interferonas Diagnóstico Animal Diseases Cattle Mycobacterium bovis Infections Antigens Interferons Diagnosis |
| url | https://www.sciencedirect.com/science/article/pii/S1472979215207688 http://hdl.handle.net/20.500.12123/4585 https://doi.org/10.1016/j.tube.2015.07.009 |
| work_keys_str_mv | AT eirinmariaemilia identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT maciasanalia identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT magnanogabriel identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT morsellaclaudiagraciela identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT mendezlauralujan identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT blancofedericocarlos identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT biancomariaveronica identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT severinawalter identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT alitoaliciaelsa identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT pandomariadelosangeles identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT singhmahavirg identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT spallekralph identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT paolicchifernando identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT bigifabiana identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis AT cataldiangeladrian identificationandevaluationofnewmycobacteriumbovisantigensintheinvitrointerferongammareleaseassayforbovinetuberculosisdiagnosis |