A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance
Endophytic bacteria colonize inner plant tissues and thrive at the apoplast, which is considered its main reservoir. Because this niche is the place where the main molecular events take place between beneficial and pathogenic microorganisms, the aim of this work was to characterize culturable endoph...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
American Phytopathological Society
2019
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4576 https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-07-18-0262-R?journalCode=phyto https://doi.org/10.1094/PHYTO-07-18-0262-R |
| _version_ | 1855483459421601792 |
|---|---|
| author | Romero, Fernando Matias Rossi, Franco Rubén Gárriz, Andrés Carrasco, Pedro Ruiz, Oscar Adolfo |
| author_browse | Carrasco, Pedro Gárriz, Andrés Romero, Fernando Matias Rossi, Franco Rubén Ruiz, Oscar Adolfo |
| author_facet | Romero, Fernando Matias Rossi, Franco Rubén Gárriz, Andrés Carrasco, Pedro Ruiz, Oscar Adolfo |
| author_sort | Romero, Fernando Matias |
| collection | INTA Digital |
| description | Endophytic bacteria colonize inner plant tissues and thrive at the apoplast, which is considered its main reservoir. Because this niche is the place where the main molecular events take place between beneficial and pathogenic microorganisms, the aim of this work was to characterize culturable endophytic bacteria from apoplastic fluids obtained from field-grown canola leaves and analyze their potential for biological control of diseases caused by Xanthomonas campestris, Sclerotinia sclerotiorum, and Leptosphaeria maculans. Dual-culture analysis indicated that three isolates (Apo8, Apo11, and Apo12) were able to inhibit the growth of all three phytopathogens. Sequencing of the 16S ribosomal RNA and rpoD genes of these isolates revealed that they are closely related to Pseudomonas viridiflava. One of the isolates, Apo11, was able to diminish the propagation of X. campestris in whole-plant assays. At the same time, Apo11 inoculation reduced the necrotic lesions provoked by S. sclerotiorum on canola leaves. This protective effect might be due to the induction of resistance in the host mediated by salicylic and jasmonic acid signaling pathways or the production of compounds with antimicrobial activity. At the same time, Apo11 inoculation promoted canola plant growth. Thus, the isolate characterized in this work has several desirable characteristics, which make it a potential candidate for the formulation of biotechnological products to control plant diseases or promote plant growth. |
| format | Artículo |
| id | INTA4576 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA45762021-07-16T14:36:28Z A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance Romero, Fernando Matias Rossi, Franco Rubén Gárriz, Andrés Carrasco, Pedro Ruiz, Oscar Adolfo Brassica Napus Endofitas Control Biológico Resistencia a la Enfermedad Endophytes Biological Control Disease Resistance Canola Apoplast Endophytic bacteria colonize inner plant tissues and thrive at the apoplast, which is considered its main reservoir. Because this niche is the place where the main molecular events take place between beneficial and pathogenic microorganisms, the aim of this work was to characterize culturable endophytic bacteria from apoplastic fluids obtained from field-grown canola leaves and analyze their potential for biological control of diseases caused by Xanthomonas campestris, Sclerotinia sclerotiorum, and Leptosphaeria maculans. Dual-culture analysis indicated that three isolates (Apo8, Apo11, and Apo12) were able to inhibit the growth of all three phytopathogens. Sequencing of the 16S ribosomal RNA and rpoD genes of these isolates revealed that they are closely related to Pseudomonas viridiflava. One of the isolates, Apo11, was able to diminish the propagation of X. campestris in whole-plant assays. At the same time, Apo11 inoculation reduced the necrotic lesions provoked by S. sclerotiorum on canola leaves. This protective effect might be due to the induction of resistance in the host mediated by salicylic and jasmonic acid signaling pathways or the production of compounds with antimicrobial activity. At the same time, Apo11 inoculation promoted canola plant growth. Thus, the isolate characterized in this work has several desirable characteristics, which make it a potential candidate for the formulation of biotechnological products to control plant diseases or promote plant growth. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Romero, Fernando Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Carrasco, Pedro. Universitat de València. Facultat de Ciències Biològiques. Departament de Bioquímica i Biologia Molecular; España Fil: Ruíz, Oscar A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina 2019-03-12T12:18:44Z 2019-03-12T12:18:44Z 2019-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4576 https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-07-18-0262-R?journalCode=phyto 0031-949X 1943-7684 (Online) https://doi.org/10.1094/PHYTO-07-18-0262-R eng info:eu-repo/semantics/restrictedAccess application/pdf American Phytopathological Society Phytopathology 109 (3) : 375–383. (March 2019) |
| spellingShingle | Brassica Napus Endofitas Control Biológico Resistencia a la Enfermedad Endophytes Biological Control Disease Resistance Canola Apoplast Romero, Fernando Matias Rossi, Franco Rubén Gárriz, Andrés Carrasco, Pedro Ruiz, Oscar Adolfo A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| title | A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| title_full | A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| title_fullStr | A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| title_full_unstemmed | A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| title_short | A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| title_sort | bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance |
| topic | Brassica Napus Endofitas Control Biológico Resistencia a la Enfermedad Endophytes Biological Control Disease Resistance Canola Apoplast |
| url | http://hdl.handle.net/20.500.12123/4576 https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-07-18-0262-R?journalCode=phyto https://doi.org/10.1094/PHYTO-07-18-0262-R |
| work_keys_str_mv | AT romerofernandomatias abacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT rossifrancoruben abacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT garrizandres abacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT carrascopedro abacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT ruizoscaradolfo abacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT romerofernandomatias bacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT rossifrancoruben bacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT garrizandres bacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT carrascopedro bacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance AT ruizoscaradolfo bacterialendophytefromapoplastfluidsprotectscanolaplantsfromdifferentphytopathogensviaantibiosisandinductionofhostresistance |