Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina
Bovine herpesvirus 4 (BoHV-4) is increasingly considered as responsible for various problems of the reproductive tract. The virus infects mainly blood mononuclear cells and displays specific tropism for vascular endothelia, reproductive and fetal tissues. Epidemiological studies suggest its impact o...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Plos One
2019
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| Subjects: | |
| Online Access: | https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0132212 http://hdl.handle.net/20.500.12123/4572 https://doi.org/10.1371/journal.pone.0132212 |
| _version_ | 1855035347473268736 |
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| author | González Altamiranda, Erika Analía Manrique, Julieta Marina Perez, Sandra Rios, Glenda Laura Odeon, Anselmo Carlos Leunda, Maria Rosa Jones, Leandro Roberto Verna, Andrea Elizabeth |
| author_browse | González Altamiranda, Erika Analía Jones, Leandro Roberto Leunda, Maria Rosa Manrique, Julieta Marina Odeon, Anselmo Carlos Perez, Sandra Rios, Glenda Laura Verna, Andrea Elizabeth |
| author_facet | González Altamiranda, Erika Analía Manrique, Julieta Marina Perez, Sandra Rios, Glenda Laura Odeon, Anselmo Carlos Leunda, Maria Rosa Jones, Leandro Roberto Verna, Andrea Elizabeth |
| author_sort | González Altamiranda, Erika Analía |
| collection | INTA Digital |
| description | Bovine herpesvirus 4 (BoHV-4) is increasingly considered as responsible for various problems of the reproductive tract. The virus infects mainly blood mononuclear cells and displays specific tropism for vascular endothelia, reproductive and fetal tissues. Epidemiological studies suggest its impact on reproductive performance, and its presence in various sites in the reproductive tract highlights its potential transmission in transfer-stage embryos. This work describes the biological and genetic characterization of BoHV-4 strains isolated from an in vitro bovine embryo production system. BoHV-4 strains were isolated in 2011 and 2013 from granulosa cells and bovine oocytes from ovary batches collected at a local abattoir, used as “starting material” for in vitro production of bovine embryos. Compatible BoHV-4-CPE was observed in the co-culture of granulosa cells and oocytes with MDBK cells. The identity of the isolates was confirmed by PCR assays targeting three ORFs of the viral genome. The phylogenetic analyses of the strains suggest that they were evolutionary unlinked. Therefore it is possible that BoHV-4 ovary infections occurred regularly along the evolution of the virus, at least in Argentina, which can have implications in the systems of in vitro embryo production. Thus, although BoHV-4 does not appear to be a frequent risk factor for in vitro embryo production, data are still limited. This study reveals the potential of BoHV-4 transmission via embryo transfer. Moreover, the high variability among the BoHV-4 strains isolated from aborted cows in Argentina highlights the importance of further research on the role of this virus as an agent with the potential to cause reproductive disease in cattle. The genetic characterization of the isolated strains provides data to better understand the pathogenesis of BoHV-4 infections. Furthermore, it will lead to fundamental insights into the molecular aspects of the virus and the means by which these strains circulate in the herds. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4572 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Plos One |
| publisherStr | Plos One |
| record_format | dspace |
| spelling | INTA45722019-03-11T14:56:17Z Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina González Altamiranda, Erika Analía Manrique, Julieta Marina Perez, Sandra Rios, Glenda Laura Odeon, Anselmo Carlos Leunda, Maria Rosa Jones, Leandro Roberto Verna, Andrea Elizabeth Ganado Bovino Enfermedades de los Animales Herpes Virus Bovino Experimentación in Vitro Embriones Animales Cattle Animal Diseases Bovine Herpesvirus In Vitro Experimentation Animal Embryos Argentina Bovine herpesvirus 4 (BoHV-4) is increasingly considered as responsible for various problems of the reproductive tract. The virus infects mainly blood mononuclear cells and displays specific tropism for vascular endothelia, reproductive and fetal tissues. Epidemiological studies suggest its impact on reproductive performance, and its presence in various sites in the reproductive tract highlights its potential transmission in transfer-stage embryos. This work describes the biological and genetic characterization of BoHV-4 strains isolated from an in vitro bovine embryo production system. BoHV-4 strains were isolated in 2011 and 2013 from granulosa cells and bovine oocytes from ovary batches collected at a local abattoir, used as “starting material” for in vitro production of bovine embryos. Compatible BoHV-4-CPE was observed in the co-culture of granulosa cells and oocytes with MDBK cells. The identity of the isolates was confirmed by PCR assays targeting three ORFs of the viral genome. The phylogenetic analyses of the strains suggest that they were evolutionary unlinked. Therefore it is possible that BoHV-4 ovary infections occurred regularly along the evolution of the virus, at least in Argentina, which can have implications in the systems of in vitro embryo production. Thus, although BoHV-4 does not appear to be a frequent risk factor for in vitro embryo production, data are still limited. This study reveals the potential of BoHV-4 transmission via embryo transfer. Moreover, the high variability among the BoHV-4 strains isolated from aborted cows in Argentina highlights the importance of further research on the role of this virus as an agent with the potential to cause reproductive disease in cattle. The genetic characterization of the isolated strains provides data to better understand the pathogenesis of BoHV-4 infections. Furthermore, it will lead to fundamental insights into the molecular aspects of the virus and the means by which these strains circulate in the herds. EEA Balcarce Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Manrique, Julieta Marina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2019-03-11T14:54:52Z 2019-03-11T14:54:52Z 2015-07-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0132212 http://hdl.handle.net/20.500.12123/4572 1932-6203 https://doi.org/10.1371/journal.pone.0132212 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos One PLoS ONE 10 (7) : e0132212 (July 2015) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Herpes Virus Bovino Experimentación in Vitro Embriones Animales Cattle Animal Diseases Bovine Herpesvirus In Vitro Experimentation Animal Embryos Argentina González Altamiranda, Erika Analía Manrique, Julieta Marina Perez, Sandra Rios, Glenda Laura Odeon, Anselmo Carlos Leunda, Maria Rosa Jones, Leandro Roberto Verna, Andrea Elizabeth Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina |
| title | Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina |
| title_full | Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina |
| title_fullStr | Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina |
| title_full_unstemmed | Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina |
| title_short | Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina |
| title_sort | molecular characterization of the first bovine herpesvirus 4 bohv 4 strains isolated from in vitro bovine embryos production in argentina |
| topic | Ganado Bovino Enfermedades de los Animales Herpes Virus Bovino Experimentación in Vitro Embriones Animales Cattle Animal Diseases Bovine Herpesvirus In Vitro Experimentation Animal Embryos Argentina |
| url | https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0132212 http://hdl.handle.net/20.500.12123/4572 https://doi.org/10.1371/journal.pone.0132212 |
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