Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV),...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0093691X16302783 http://hdl.handle.net/20.500.12123/4570 https://doi.org/10.1016/j.theriogenology.2016.06.020 |
| _version_ | 1855483458270265344 |
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| author | González Altamiranda, Erika Analía Kaiser, German Gustavo Rios, Glenda Laura Leunda, Maria Rosa Odeon, Anselmo Carlos |
| author_browse | González Altamiranda, Erika Analía Kaiser, German Gustavo Leunda, Maria Rosa Odeon, Anselmo Carlos Rios, Glenda Laura |
| author_facet | González Altamiranda, Erika Analía Kaiser, German Gustavo Rios, Glenda Laura Leunda, Maria Rosa Odeon, Anselmo Carlos |
| author_sort | González Altamiranda, Erika Analía |
| collection | INTA Digital |
| description | Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection. |
| format | Artículo |
| id | INTA4570 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45702019-03-11T14:29:04Z Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells González Altamiranda, Erika Analía Kaiser, German Gustavo Rios, Glenda Laura Leunda, Maria Rosa Odeon, Anselmo Carlos Enfermedades de los Animales Ganado Bovino Diarrea Viral Bovina Pestivirus de la Diarrea Bovina Óvulo Animal Diseases Cattle Bovine Viral Diarrhoea Bovine Diarrhoea Pestivirus Ova Ovocitos Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection. EEA Balcarce Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2019-03-11T14:25:43Z 2019-03-11T14:25:43Z 2016-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0093691X16302783 http://hdl.handle.net/20.500.12123/4570 0093-691X 1879-3231 https://doi.org/10.1016/j.theriogenology.2016.06.020 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Theriogenology 86 (8) : 1999-2003 (November 2016) |
| spellingShingle | Enfermedades de los Animales Ganado Bovino Diarrea Viral Bovina Pestivirus de la Diarrea Bovina Óvulo Animal Diseases Cattle Bovine Viral Diarrhoea Bovine Diarrhoea Pestivirus Ova Ovocitos González Altamiranda, Erika Analía Kaiser, German Gustavo Rios, Glenda Laura Leunda, Maria Rosa Odeon, Anselmo Carlos Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells |
| title | Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells |
| title_full | Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells |
| title_fullStr | Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells |
| title_full_unstemmed | Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells |
| title_short | Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells |
| title_sort | interaction of bovine viral diarrhea virus with bovine cumulus oocyte complex during ivm detection in permissive cells |
| topic | Enfermedades de los Animales Ganado Bovino Diarrea Viral Bovina Pestivirus de la Diarrea Bovina Óvulo Animal Diseases Cattle Bovine Viral Diarrhoea Bovine Diarrhoea Pestivirus Ova Ovocitos |
| url | https://www.sciencedirect.com/science/article/pii/S0093691X16302783 http://hdl.handle.net/20.500.12123/4570 https://doi.org/10.1016/j.theriogenology.2016.06.020 |
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