Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals we...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Botanical Society of America
2019
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| Acceso en línea: | https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120 http://hdl.handle.net/20.500.12123/4555 https://doi.org/10.3732/apps.1400120 |
| _version_ | 1855483455475810304 |
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| author | Bozzi, Jorge Alfredo Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
| author_browse | Bozzi, Jorge Alfredo Gallo, Leonardo Ariel Leyer, Ilona Liepelt, Sascha Marchelli, Paula Mengel, Christina Ohneiser, Sebastian Ziegenhagen, Birgit |
| author_facet | Bozzi, Jorge Alfredo Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
| author_sort | Bozzi, Jorge Alfredo |
| collection | INTA Digital |
| description | Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing.
Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus.
Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. |
| format | Artículo |
| id | INTA4555 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Botanical Society of America |
| publisherStr | Botanical Society of America |
| record_format | dspace |
| spelling | INTA45552019-03-08T12:51:54Z Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species Bozzi, Jorge Alfredo Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina Salix Árboles Maderables Marcadores Genéticos Secuencia Nucleotídica Timber Trees Genetic Markers Nucleotide Sequence Salix humboldtiana Marcadores Moleculares Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. EEA Bariloche Fil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania Fil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; Alemania Fil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania Fil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania 2019-03-08T12:47:02Z 2019-03-08T12:47:02Z 2015-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120 http://hdl.handle.net/20.500.12123/4555 1550-7416 https://doi.org/10.3732/apps.1400120 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Botanical Society of America Applications in Plant Sciences 3 (4) : 1400120 ( April 2015) |
| spellingShingle | Salix Árboles Maderables Marcadores Genéticos Secuencia Nucleotídica Timber Trees Genetic Markers Nucleotide Sequence Salix humboldtiana Marcadores Moleculares Bozzi, Jorge Alfredo Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
| title | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
| title_full | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
| title_fullStr | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
| title_full_unstemmed | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
| title_short | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
| title_sort | characterization of 23 polymorphic ssr markers in salix humboldtiana salicaceae using next generation sequencing and cross amplification from related species |
| topic | Salix Árboles Maderables Marcadores Genéticos Secuencia Nucleotídica Timber Trees Genetic Markers Nucleotide Sequence Salix humboldtiana Marcadores Moleculares |
| url | https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120 http://hdl.handle.net/20.500.12123/4555 https://doi.org/10.3732/apps.1400120 |
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