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Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species

Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals we...

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Main Authors: Bozzi, Jorge Alfredo, Liepelt, Sascha, Ohneiser, Sebastian, Gallo, Leonardo Ariel, Marchelli, Paula, Leyer, Ilona, Ziegenhagen, Birgit, Mengel, Christina
Format: Artículo
Language:Inglés
Published: Botanical Society of America 2019
Subjects:
Salix
Árboles Maderables
Marcadores Genéticos
Secuencia Nucleotídica
Timber Trees
Genetic Markers
Nucleotide Sequence
Salix humboldtiana
Marcadores Moleculares
Online Access:https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120
http://hdl.handle.net/20.500.12123/4555
https://doi.org/10.3732/apps.1400120
Summary:Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.

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